Genome-wide identification, classification and expression pattern of LBD gene family inCamellia sinensis

Abstract

The plant-specific LBD (LATERAL ORGAN BOUNDARIES DOMAIN) genes belong to a major family of transcription factors that encode a zinc finger-like domain. This family plays crucial roles in plant development, including lateral organ development, pathogen response, pollen development, plant regeneration and hormone response. However, there is no detailed information of LBD genes in the tea plant. Tea plant [Camellia sinensis (L.) O. Kuntze] is a perennial evergreen woody crop that is cultivated worldwide. Tea leaves are used as a non-alcoholic beverage, which is known for its beneficial ingredients and multiple flavours. In this study, a total of 31 CsLBD genes were identified and characterized based on the genome of C. sinensis. CsLBD factors and Arabidopsis counterparts were clustered into 10 subgroups after phylogenetic analysis. Conserved motif analysis revealed that CsLBD proteins with a close evolutionary relationship possessed uniform or similar motif compositions. The interaction networks of LBD proteins in C. sinensis were established according to the data in Arabidopsis. Seven CsLBD genes were examined through qRT-PCR (quantitative real time polymerase chain reaction) in tea plant cultivar, 'Longjing43'. The expression profiles of these CsLBD genes were investigated in different tissues (i.e. root, stem, leaf and flower) and subjected to five hormonal stimuli (i.e. abscisic acid, gibberellin acid, ethephon, methyl jasmonate and salicylic acid). This study provides an extensive overview of the CsLBD family genes and a valuable foundation for future functional studies of CsLBD factors.

Comprehensive characterization and gene expression patterns of LBD gene family in Gossypium

“…LBD genes comprise a plant-specific transcription factor family, which had been proven to have essential roles in the regulation of lateral organ development and metabolic processes, such as anthocyanin and nitrogen metabolism in higher plants (Iwakawa et al 2002;Shuai et al 2002;Majer and Hochholdinger 2011). Comprehensive analyses of this gene family had been conducted in many species such as Arabidopsis (Liu et al 2005), Eucalyptus grandis (Lu et al 2018), Brassica rapa (Huang et al 2018), grapevine (Grimplet et al 2017), soybean (Yang et al 2017), and Camellia sinensis (Teng et al 2018). However, no systematic study of LBD genes has been conducted in allotetraploid cotton.…”

“…In Arabidopsis, LBD40 was reported to be down-regulated by gibberellin (Zentella et al 2007), LBD20 was proposed playing a role in JA-response (Thatcher et al 2012a). In Camellia sinensis, LBD genes had differential functions in response to the treatments with gibberellic acid (GA3), salicylic acid (SA), MeJA, ABA, and ethephon (Eth) (Teng et al 2018). Considering that LBD genes are conservative in structure and function in different species (Wang et al 2013;Yang et al 2016), and the results of Cis-acting element in promoters of GhLBDs and GbLBDs leaded us to draw the predication that the LBDs in cotton were involved in phytohormone responses and stress resistance.…”

Identification ofBrassica oleraceaorthologs of the PEBP family and their expression patterns in curd development and flowering in cauliflower

“…The MEME online software was previously reported to be used to identify conserved motifs of different protein sequences [43,44]. Figure 2 shows the PEBP protein structures from B. oleracea and A. thaliana investigated by MEME.…”

RNAi of a Putative Grapevine Susceptibility Gene as a Possible Downy Mildew Control Strategy

“…The S-gene VvLBDp1 [from here on called VviLBDIf7 based on Grimplet et al (2017) nomenclature], encoding for an LOB (LATERAL ORGAN BOUNDARIES) domain-containing (LBD) protein, belongs to plant LOB family of transcription factors. This family has been comprehensively analyzed in many species, such as Arabidopsis thaliana ( Liu et al, 2005 ), Malus domestica ( Wang et al, 2013 ), Glycine max ( Yang et al, 2017 ), Eucalyptus grandis ( Lu et al, 2018 ), Brassica rapa ( Huang et al, 2018 ), Camellia sinensis ( Teng et al, 2018 ), Gossypium spp. ( Yu et al, 2020 ), and Pyrus bretschneideri ( Song et al, 2020 ).…”