Genome-wide identification, evolution, and expression analysis of the<i>KT/HAK/KUP</i>family in pear

Wang, Yingzhen; Lü, Jiahong; Chen, Dan; Zhang, Jun; Qi, Kaijie; Cheng, Rui; Zhang, Huping; Zhang, Shaoling

doi:10.1139/gen-2017-0254

Cited by 27 publications

(26 citation statements)

References 44 publications

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“…Low-K + stress tends to induce the upregulated expression of K + transporter genes [40]. Previous studies demonstrated that the expression of OsHAK1 in rice [20], TaHAK1 in wheat [36] and PbrHAK1 in pear [41] was induced by K + starvation. In this study, the expression level of SsHAK1 increased rapidly under low-K + stress, and this result is in good agreement with previous studies.…”

Section: Gene Expression and Functional Divergence Of Haks In Saccharummentioning

confidence: 99%

Genome-wide systematic characterization of the HAK/KUP/KT gene family and its expression profile during plant growth and in response to low-K+ stress in Saccharum

Feng¹,

Wang

Zhang³

et al. 2020

BMC Plant Biol

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Background: Plant genomes contain a large number of HAK/KUP/KT transporters, which play important roles in potassium uptake and translocation, osmotic potential regulation, salt tolerance, root morphogenesis and plant development. Potassium deficiency in the soil of a sugarcane planting area is serious. However, the HAK/KUP/KT gene family remains to be characterized in sugarcane (Saccharum). Results: In this study, 30 HAK/KUP/KT genes were identified in Saccharum spontaneum. Phylogenetics, duplication events, gene structures and expression patterns were analyzed. Phylogenetic analysis of the HAK/KUP/KT genes from 15 representative plants showed that this gene family is divided into four groups (clades I-IV). Both ancient wholegenome duplication (WGD) and recent gene duplication contributed to the expansion of the HAK/KUP/KT gene family. Nonsynonymous to synonymous substitution ratio (Ka/Ks) analysis showed that purifying selection was the main force driving the evolution of HAK/KUP/KT genes. The divergence time of the HAK/KUP/KT gene family was estimated to range from 134.8 to 233.7 Mya based on Ks analysis, suggesting that it is an ancient gene family in plants. Gene structure analysis showed that the HAK/KUP/KT genes were accompanied by intron gain/loss in the process of evolution. RNA-seq data analysis demonstrated that the HAK/KUP/KT genes from clades II and III were mainly constitutively expressed in various tissues, while most genes from clades I and IV had no or very low expression in the tested tissues at different developmental stages. The expression of SsHAK1 and SsHAK21 was upregulated in response to low-K + stress. Yeast functional complementation analysis revealed that SsHAK1 and SsHAK21 could rescue K + uptake in a yeast mutant. Conclusions: This study provided insights into the evolutionary history of HAK/KUP/KT genes. HAK7/9/18 were mainly expressed in the upper photosynthetic zone and mature zone of the stem. HAK7/9/18/25 were regulated by sunlight. SsHAK1 and SsHAK21 played important roles in mediating potassium acquisition under limited K + supply. Our results provide valuable information and key candidate genes for further studies on the function of HAK/KUP/KT genes in Saccharum.

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Section: Gene Expression and Functional Divergence Of Haks In Saccharummentioning

confidence: 99%

Genome-wide systematic characterization of the HAK/KUP/KT gene family and its expression profile during plant growth and in response to low-K+ stress in Saccharum

Feng¹,

Wang

Zhang³

et al. 2020

BMC Plant Biol

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“…AtKC1 appears to be a regulatory subunit for AtAKT1 in root hairs [19,48]. KUP/HAK/KTs are identified to be high-affinity K + transporters and play a role in the homeostasis of K + in cells [50][51][52][53][54][55]. AtHAK5 was expressed in Arabidopsis roots, and it regulated most of the K + uptake in Arabidopsis roots [52], and the high expression of the AtHAK5 gene could be induced by K + deficiency and low K + levels in Arabidopsis roots to adapt to the low K + environment [23].…”

Section: Discussionmentioning

confidence: 99%

Multiple High-Affinity K+ Transporters and ABC Transporters Involved in K+ Uptake/Transport in the Potassium-Hyperaccumulator Plant Phytolacca acinosa Roxb

Xie

Tan

Deng

et al. 2020

Plants

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Potassium is an important essential element for plant growth and development. Long-term potassium deprivation can lead to a severe deficiency phenotype in plants. Interestingly, Phytolacca acinosa is a plant with an unusually high potassium content and can grow well and complete its lifecycle even in severely potassium deficient soil. In this study, we found that its stems and leaves were the main tissues for high potassium accumulation, and P. acinosa showed a strong ability of K+ absorption in roots and a large capability of potassium accumulation in shoots. Analysis of plant growth and physiological characteristics indicated that P. acinosa had an adaptability in a wide range of external potassium levels. To reveal the mechanism of K+ uptake and transport in the potassium-hyperaccumulator plant P. acinosa, K+ uptake-/transport-related genes were screened by transcriptome sequencing, and their expression profiles were compared between K+ starved plants and normal cultured plants. Eighteen members of HAK/KT/KUPs, ten members of AKTs, and one member of HKT were identified in P. acinosa. Among them, six HAKs, and two AKTs and PaHKT1 showed significantly different expression. These transporters might be coordinatively involved in K+ uptake/transport in P. acinosa and lead to high potassium accumulation in plant tissues. In addition, significantly changed expression of some ABC transporters indicated that ABC transporters might be important for K+ uptake and transport in P. acinosa under low K+ concentrations.

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“…Low K + stress tends to induce the upregulated expression of K + transporter genes [40]. previous studies demonstrated that the expression of OsHAK1 in rice [20], TaHAK1 in wheat [36] and PbrHAK1 in pear [41] were induced by K + starvation. In this study, the expression level of SsHAK1 increased rapidly under low K + stress, and this result is in good agreement with previous studies.…”

Section: Gene Expression and Functional Divergence Of Haks In Saccharrummentioning

confidence: 95%

Genome-wide systematic characterization of HAK/KUP/KT gene family and their expression profiles during plant growth and in response to low K+ stress in Saccharum

Feng

Wang

Zhang

et al. 2019

Preprint

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Background: Plant genomes contain large number of HAK/KUP/KT transporters, and they play important roles in potassium uptake and translocation, osmotic potential regulation, salt tolerance, root morphogenesis and plant development. Potassium deficiency in soil of sugarcane main planting area is serious. However, the HAK/KUP/KT gene family remains to be characterized in sugarcane (Saccharum). Results: In this study, 30 HAK/KUP/KT genes were identified from Saccharum spontaneum. Phylogenetics, duplication events, gene structure and expression pattern were analyzed. Phylogenetic analysis of HAK/KUP/KT genes from 15 representative plants showed that this gene family were divided into four groups (clade I-IV). Both ancient whole-genome duplication (WGD) and recent gene duplication contributed to the expansion of HAK/KUP/KT gene family. Nonsynonymous to synonymous substitution ratio (Ka/Ks) analysis showed that purifying selection was the main force to drive the evolution of HAK/KUP/KT genes. The divergence time of HAK/KUP/KT gene family was estimated to range from 134.8 to 233.7 Mya based on Ks analysis, suggesting that it is an ancient gene family in plants. Gene structure analysis showed that HAK/KUP/KT genes was accompanied by intron gain/loss in the process of evolution. RNA-seq data analysis demonstrated that HAK/KUP/KT genes from clade II and III mainly displayed constitutive expression in various tissues, while most genes from clade I and IV had no or very low expression in the tested tissues at different developmental stages. SsHAK1 and SsHAK21 displayed upregulated expression in response to low K+ stress. Yeast functional complementation analysis suggested that SsHAK1 and SsHAK21 could rescue K+ uptake ability in the yeast mutant. Conclusions: This study provided insight into the gene evolutionary history of HAK/KUP/KT genes. HAK7/9/18 were mainly expressed in the high photosynthetic zone and mature zone of stem. Moreover HAK7/9/18/25 were regulated by sunlight. SsHAK1 and SsHAK21 played important role in mediating potassium acquisition under limited K+ supply. Our results provide valuable information and key candidate genes for further study on the function of HAK/KUP/KT genes in Saccharum. Keywords: Saccharum, HAK/KUP/KT, evolution, gene expression, low K+ stress

show abstract

Genome-wide identification, evolution, and expression analysis of theKT/HAK/KUPfamily in pear

Cited by 27 publications

References 44 publications

Genome-wide systematic characterization of the HAK/KUP/KT gene family and its expression profile during plant growth and in response to low-K+ stress in Saccharum

Genome-wide systematic characterization of the HAK/KUP/KT gene family and its expression profile during plant growth and in response to low-K+ stress in Saccharum

Multiple High-Affinity K+ Transporters and ABC Transporters Involved in K+ Uptake/Transport in the Potassium-Hyperaccumulator Plant Phytolacca acinosa Roxb

Genome-wide systematic characterization of HAK/KUP/KT gene family and their expression profiles during plant growth and in response to low K+ stress in Saccharum

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