Genome-wide identification, expression signature and immune functional analysis of two cathepsin S (CTSS) genes in turbot (Scophthalmus maximus L.)

Fu, Qiang; Zhao, Shoucong; Tian, Mengyu; Cai, Xin; Zhang, Lu; Hu, Jie; Cao, Min; Xue, Ting; Li, Chao

doi:10.1016/j.fsi.2020.04.028

Cited by 8 publications

(3 citation statements)

References 60 publications

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“…Under LPS stimulation, the expression of ctss increased. Lysosome rupture accelerates the diffusion of CTSS to the cytoplasm and increases cell death [32]. Cathepsin D (CTSD) is an aspartic protease, which is involved in lysosomal digestion, protein synthesis, and activation [33].…”

Section: Discussionmentioning

confidence: 99%

“…The subsequent qRT-PCR validation in zebrafish whole embryos further revealed that the abnormal expression of Toll-like receptor signaling (ikbkb, ifnphi3, nfkb1, and stat2) and related inflammation cytokines and chemokines (cxcl18b, ccl19b, btk, and tnfb) in the LPS group was significantly restored to normal expression levels in varying degrees after the DSF treatment when compared with the expressions of these genes in control embryos (Figure 7D-E). Meanwhile, when detecting the expression of lysosomeapoptosis-related genes, such as the lysosomal acid hydrolases ctss 2.1 and ctsd, which are related to the synthesis of cathepsin and participate in the apoptotic process [32,33], gzm3.4, which encodes a protein that can activate the caspase chain reaction and lead to apoptosis [34,35], and casp7, from a family of protease enzymes playing important roles in programmed cell death [36], the abnormal expression of these genes was restored in the DSF + LPS group compared with the LPS group (Figure 7F). These findings elucidated the potential mechanism of DSF in the treatment of LPS-induced inflammation and apoptosis by inhibiting the activation of signaling in the Toll-like receptor pathway, attenuating the expression of pro-inflammatory cytokines and chemokines, and regulating the activation of the caspase cascade by restoring the expression of lysosomal cathepsins and apoptosis signaling (Figure 8).…”

Section: Dsf Inhibits Lps-induced Inflammation Through Toll-like Rece...mentioning

confidence: 99%

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Bacterial Quorum-Sensing Signal DSF Inhibits LPS-Induced Inflammations by Suppressing Toll-like Receptor Signaling and Preventing Lysosome-Mediated Apoptosis in Zebrafish

Zhu

Wang

et al. 2022

IJMS

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Bacteria and their eukaryotic hosts have co-evolved for millions of years, and the former can intercept eukaryotic signaling systems for the successful colonization of the host. The diffusible signal factor (DSF) family represents a type of quorum-sensing signals found in diverse Gram-negative bacterial pathogens. Recent evidence shows that the DSF is involved in interkingdom communications between the bacterial pathogen and the host plant. In this study, we explored the anti-inflammatory effect of the DSF and its underlying molecular mechanism in a zebrafish model. We found that the DSF treatment exhibited a strong protective effect on the inflammatory response of zebrafish induced by lipopolysaccharide (LPS). In the LPS-induced inflammation zebrafish model, the DSF could significantly ameliorate the intestinal pathological injury, reduce abnormal migration and the aggregation of inflammatory cells, inhibit the excessive production of inflammatory mediator reactive oxygen species (ROS) content, and prevent apoptosis. Through an RNA-Seq analysis, a total of 938 differentially expressed genes (DEGs) was screened between LPS and LPS + DSF treatment zebrafish embryos. A further bioinformatics analysis and validation revealed that the DSF might inhibit the LPS-induced zebrafish inflammatory response by preventing the activation of signaling in the Toll-like receptor pathway, attenuating the expression of pro-inflammatory cytokines and chemokines, and regulating the activation of the caspase cascade through restoring the expression of lysosomal cathepsins and apoptosis signaling. This study, for the first time, demonstrates the anti-inflammatory role and a potential pharmaceutical application of the bacterial signal DSF. These findings also suggest that the interkingdom communication between DSF-producing bacteria and zebrafish might occur in nature.

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Section: Discussionmentioning

confidence: 99%

Section: Dsf Inhibits Lps-induced Inflammation Through Toll-like Rece...mentioning

confidence: 99%

Bacterial Quorum-Sensing Signal DSF Inhibits LPS-Induced Inflammations by Suppressing Toll-like Receptor Signaling and Preventing Lysosome-Mediated Apoptosis in Zebrafish

Zhu

Wang

et al. 2022

IJMS

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“…To determine the PPI network of expressed proteins, amino acid sequences of cathepsins were blasted against C. semilaevis or D. rerio by using STRING software 11.0. Representation of the protein–protein network was analyzed at a confidence score of 0.40 from text mining, experiments, databases, co-expression, and neighborhood sources [ 70 ].…”

Section: Methodsmentioning

confidence: 99%

Genome-Wide Identification, Evolutionary Analysis, and Expression Patterns of Cathepsin Superfamily in Black Rockfish (Sebastes schlegelii) following Aeromonas salmonicida Infection

Zhang

Chen

et al. 2022

Marine Drugs

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Cathepsins are lysosomal cysteine proteases belonging to the papain family and play crucial roles in intracellular protein degradation/turnover, hormone maturation, antigen processing, and immune responses. In the present study, 18 cathepsins were systematically identified from the fish S. schlegelii genome. Phylogenetic analysis indicated that cathepsin superfamilies are categorized into eleven major clusters. Synteny and genome organization analysis revealed that whole-genome duplication led to the expansion of S. schlegelii cathepsins. Evolutionary rate analyses indicated that the lowest Ka/Ks ratios were observed in CTSBa (0.13) and CTSBb (0.14), and the highest Ka/Ks ratios were observed in CTSZa (1.97) and CTSZb (1.75). In addition, cathepsins were ubiquitously expressed in all examined tissues, with high expression levels observed in the gill, intestine, head kidney, and spleen. Additionally, most cathepsins were differentially expressed in the head kidney, gill, spleen, and liver following Aeromonas salmonicida infection, and their expression signatures showed tissue-specific and time-dependent patterns. Finally, protein–protein interaction network (PPI) analyses revealed that cathepsins are closely related to a few immune-related genes, such as interleukins, chemokines, and TLR genes. These results are expected to be valuable for comparative immunological studies and provide insights for further functional characterization of cathepsins in fish species.

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CC chemokines and their receptors in black rockfish (Sebastes schlegelii): Characterization, evolutionary analysis, and expression patterns after Aeromonas Salmonicida infection

Zhao

et al. 2022

Aquaculture

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Genome-wide identification, expression signature and immune functional analysis of two cathepsin S (CTSS) genes in turbot (Scophthalmus maximus L.)

Cited by 8 publications

References 60 publications

Bacterial Quorum-Sensing Signal DSF Inhibits LPS-Induced Inflammations by Suppressing Toll-like Receptor Signaling and Preventing Lysosome-Mediated Apoptosis in Zebrafish

Bacterial Quorum-Sensing Signal DSF Inhibits LPS-Induced Inflammations by Suppressing Toll-like Receptor Signaling and Preventing Lysosome-Mediated Apoptosis in Zebrafish

Genome-Wide Identification, Evolutionary Analysis, and Expression Patterns of Cathepsin Superfamily in Black Rockfish (Sebastes schlegelii) following Aeromonas salmonicida Infection

CC chemokines and their receptors in black rockfish (Sebastes schlegelii): Characterization, evolutionary analysis, and expression patterns after Aeromonas Salmonicida infection

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