Genome‐wide identification of a novel mi<scp>RNA</scp>‐based signature to predict recurrence in patients with gastric cancer

Yang, Yongmei; Qu, Ailin; Zhao, Rui; Hua, Mengmeng; Zhang, Xin; Dong, Zhaogang; Zheng, Guixi; Wang, Hongchun; Yang, Xiaoyun; Zhang, Yi

doi:10.1002/1878-0261.12385

Cited by 28 publications

(24 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…54 Recently, several research groups have established new predictive models to more accurately and quickly predict the prognosis of gastric cancer patients. 55,56 In this study, nine lncRNAs (AC018647.3, MAGI2-AS3, MIR99AHG, NR2F1-AS1, LINC00106, PVT1, RP5-1074L1.4, RP11-7K24.3, and RP11-999E24.3) were found to be associated with the overall survival of patients. Among these lncRNAs, PVT1 is one of the best studied lncRNAs.…”

Section: Discussionmentioning

confidence: 60%

Integrated analysis of a ceRNA network reveals potential prognostic lncRNAs in gastric cancer

et al. 2020

Cancer Medicine

View full text Add to dashboard Cite

Long noncoding RNAs (lncRNAs) have important biological functions as competing endogenous RNAs (ceRNAs) in tumors, yet the functions and regulatory mechanisms of lncRNA‐related ceRNAs in gastric cancer have not been fully elucidated. In this study, we constructed a lncRNA‐miRNA‐mRNA ceRNA network and identified potential lncRNA biomarkers in gastric cancer. Basing on the RNA profiles downloaded from The Cancer Genome Atlas (TCGA) platform, the gastric cancer‐specific differentially expressed lncRNAs, miRNAs, and mRNAs were screened for constructing a ceRNA network using bioinformatic tools. The enrichment analysis of the biological processes in Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes pathways was performed on the ceRNA‐related DEmRNAs. According to the modularization of protein‐protein interaction (PPI) network, we extracted a ceRNA subnetwork and analyzed the correlation between the expression of the lncRNAs involved and specific clinical features of patients. Next, the expression of highly up‐regulated in liver cancer (HULC) and RP11‐314B1.2 showed significant changes in several pathological processes involved in gastric cancer, and nine lncRNAs were found to be correlated with the overall survival of patients with gastric cancer. Through the univariate and multivariate Cox regression analyses, two lncRNAs (LINC00106 and RP11‐999E24.3) were identified and utilized to establish a risk score model for assessing the prognosis of patients. The analysis results were also partially verified using quantitative real‐time PCR. The findings from this study indicate that HULC, RP11‐314B1.2, LINC00106, and RP11‐999E24.3 could be considered as potential therapeutic targets or prognostic biomarkers in gastric cancer, and provide a new perspective for cancer pathogenesis research.

show abstract

Section: Discussionmentioning

confidence: 60%

Integrated analysis of a ceRNA network reveals potential prognostic lncRNAs in gastric cancer

et al. 2020

Cancer Medicine

View full text Add to dashboard Cite

show abstract

“…Although the early diagnosis and therapy of GC were rapidly developed over the past decades, most of the patients with GC were faced with metastasis recurrence [31][32][33]. It is important for GC patients to search out effective new biomarkers.…”

Section: Discussionmentioning

confidence: 99%

MiR-876-5p regulates gastric cancer cell proliferation, apoptosis and migration through targeting WNT5A and MITF

Qing-hong

et al. 2019

Bioscience Reports

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are reported to play critical roles in various cancers. Recently, mounting miRNAs are found to exert oncogenic or tumor inhibitory role in gastric cancer (GC), however, their potential molecular mechanism in GC remains ill-defined. Currently, we aimed to elucidate the functional and mechanistic impacts of a novel miRNA on GC cellular process. The significant down-regulation of miR-876-5p in GC cells attracted our attention. In function, we performed gain-of-function assays and found that miR-876-5p overexpression repressed proliferative, anti-apoptotic and migratory abilities and epithelial–mesenchymal transition (EMT) of GC cells. By applying bioinformatics prediction and mechanism experiments, we verified that miR-876-5p could double-bind to the 3′ untranslated regions (3′UTRs) of Wnt family member 5A (WNT5A) and melanogenesis associated transcription factor (MITF), thus regulating their mRNA and protein levels. Both WNT5A and MITF were highly expressed in GC cells. Additionally, we conducted loss-of-function assays and confirmed the oncogenic roles of WNT5A and MITF in GC. Finally, rescue assay uncovered a fact that miR-876-5p suppressed GC cell viability and migration, but induced cell apoptosis via targeting WNT5A and MITF. Taken together, we might offer a valuable evidence for miR-876-5p role in GC development.

show abstract

“…Some existing studies also explored the way hsa-mir-3923 affects the occurrence and development of tumor 7,39 . According to large-scale clinical statistics, its obvious high-expression phenomenon was identified in the development of some tumor cell lines 8,40 .…”

Section: Discussionmentioning

confidence: 99%

MicroRNA hsa-mir-3923 serves as a diagnostic and prognostic biomarker for gastric carcinoma

Yang

Zhang

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Gastric carcinoma (GC) refers to a common digestive system disease that exhibits a very high incidence. MicroRNA hsa-mir-3923 belongs to a type of miRNA, of which the function has been merely investigated in breast, pancreatic cancers and pre-neoplasic stages of gastric cancer. It has not been studied or reported in gastric carcinoma, so the relationship between gastric hsa-mir-3923 expression and the clinics feature and pathology of GC cases was examined. This study employed data mining for analyzing gastric carcinoma data in The Cancer Genome Atlas database. A Chi squared test was performed for assessing the relations of hsa-mir-3923 expression with clinics-related and pathologyregulated variables. This study conducted the assessment of the role of hsa-mir-3923 in prognostic process using Kaplan-Meier curves, Receiver operating characteristic (ROC) analysis and proportional hazards model (Cox) study. With the use of Gene Expression Omnibus, this study carried out gene set enrichment analysis (GSEA). In the meantime, the common miRNA database was compared to predict potential target genes; as revealed by co-expression analysis, a regulatory network probably existed, containing hsa-mir-3923. For the analysis of the most tightly associated cytological behavior and pathway in GC, this study adopted the databases for Annotation, Visualization and Integrated Discovery (David) and KO-Based Annotation System (KOBAS). Cytoscape, R and STRING were employed for mapping probable regulatory networks displaying relations to hsa-mir-3923. Lastly, we obtained 69 genes most tightly associated with hsa-mir-3923 and described their relationship with Circos plot. As revealed from the results, hsa-mir-3923 displayed up-regulation in gastric carcinoma, and it displayed associations with vital status, N stage and histologic grade when being expressed. The predicted results of miRNA target genes suggested that there may be a close relationship between 66 genes and hsa-mir-3923 in gastric cancer. As indicated from co-expression data, a small regulating network of 4 genes probably existed. Our results elucidated that hsa-mir-3923 high-expression reveals poor prognosis of GC patients. Gastric carcinoma (GC) refers to a type of common malignant tumor in digestive tract. It exhibits high mortality and poor prognosis 1. Optimized treatment (e.g., effective surgery and neoadjuvant treatment) cannot enhance its overall survival (OS) rate 2. Thus, novel biomarkers specific to GC should be identified for disease detection in early stage. This effort can be beneficial to start the early treatment of GC and enhance the prognosis of patients. MicroRNA can noticeably affect the study on cancer in terms of tumor's biological activity (e.g., tumor transcription, epigenetic regulation, as well as expression of gene) 3. They are capable of interacting with mRNA or lncRNA, leading to the formation of pathway in human cancers 4. As demonstrated by a number of examples, the interaction between lncRNA OIP5-AS1 and miR-186a can inhibit ZEB1 expression in he...

show abstract

Genome‐wide identification of a novel miRNA‐based signature to predict recurrence in patients with gastric cancer

Cited by 28 publications

References 51 publications

Integrated analysis of a ceRNA network reveals potential prognostic lncRNAs in gastric cancer

Integrated analysis of a ceRNA network reveals potential prognostic lncRNAs in gastric cancer

MiR-876-5p regulates gastric cancer cell proliferation, apoptosis and migration through targeting WNT5A and MITF

MicroRNA hsa-mir-3923 serves as a diagnostic and prognostic biomarker for gastric carcinoma

Contact Info

Product

Resources

About