Genome-Wide Identification of ATG Gene Family Members in Fagopyrum tataricum and Their Expression during Stress Responses

Abstract

Abiotic stresses such as drought and salinity are major environmental factors limiting plant productivity. Autophagy-related genes are extensively involved in plant growth, development, and adverse stress responses, which have not yet been characterized in Tartary buckwheat (Fagopyrum tataricum, TB). In this study, we verified that drought stress could induce autophagy in TB roots. Next, 49 FtATGs in the whole genome of TB were identified. All FtATGs were randomly distributed in 8 known chromosomes, while 11 FtATGs were predictably segmental repeats. As the core component of autophagy, there were 8 FtATG8s with similar gene structures in TB, while FtATG8s showed high expression at the transcription level under drought and salt stresses. The cis-acting element analysis identified that all FtATG8 promoters contain light-responsive and MYB-binding elements. FtATG8s showed a cell-wide protein interaction network and strongly correlated with distinct stress-associated transcription factors. Furthermore, overexpression of FtATG8a and FtATG8f enhanced the antioxidant enzyme activities of TB under adverse stresses. Remarkably, FtATG8a and FtATG8f may be vital candidates functioning in stress resistance in TB. This study prominently aids in understanding the biological role of FtATG genes in TB.

Genome-wide characterization and expression analysis of MADS-box transcription factor gene family in Perilla frutescens

“…The most common P. frutescens varieties grown in the northwest region of China, such as Shanxi and other places, are primarily cultivated for oil production. However, field cultivation of P. frutescens in this region often faces challenges such as low temperature and drought stresses during the seedling and reproductive growth stages ( Fang et al, 2022 ; Zhao et al., 2021 ). Currently, there is a limited number of studies investigating the molecular mechanism of stress responses, substance synthesis, and metabolism regulation in P. frutescens .…”

The Role of Plant Ubiquitin-like Modifiers in the Formation of Salt Stress Tolerance

“…Among them, the eight FtATG8 genes showed a high expression level under both salt and drought stresses. Also, all FtATG8 genes contained MYB-family transcription factors binding and light-responsive cis-acting elements, and strongly correlated with other stress-associated transcription factors, suggesting their vital role in stress resistance in buckwheat plants [ 108 ].…”