Genome-Wide Identification of B3 DNA-Binding Superfamily Members (ABI, HIS, ARF, RVL, REM) and Their Involvement in Stress Responses and Development in Camelina sativa

Abstract

The B3 DNA-binding superfamily is a group of gene families that contain a B3 domain in their proteins. Members of this superfamily are involved in responses to stresses including salt, drought, and cold stress. The B3 DNA-binding superfamily has not been fully studied in Camelina sativa. A total of eighty-seven members of this superfamily were identified in C. sativa. The identified genes were placed into five groups based on a phylogenetic analysis. All the proteins were predicted to be nuclear. The RAV and ARF gene family had the most conserved motifs, with nine out of ten motifs being preserved, while the REM gene family was discovered to have the fewest, with just one conserved motif being present. The RAV and REM gene families showed the least protein–protein interactions. The CsARF5 and CsARF7 genes showed the highest potent interaction score with multiple auxin-responsive proteins. A qPCR analysis was carried out on six genes that showed stress-induced expression changes. CsREM17, CsREM5, and CsRAVL5 were discovered to be considerably increased in response to drought stress, while CsARF10, CsARF4, and CsREM34 were found to be downregulated to a large extent. The B3 DNA-binding superfamily regulates abscisic acid signaling, which in turn influences plant growth and stress resistance.

Genome-wide characterization and expression profiling of E2F/DP gene family members in response to abiotic stress in tomato (Solanum lycopersicum L.)

“…Several genes involved in the co-expression network interaction were related to abiotic stress tolerance. SlE2F/DP3 co-expressed genes such as SlHSP SlLRR, SlF-box-LRR, NAC domain gene and B3 DNA binding domain were reported against biotic and abiotic stress [ 19 , 34 , 63 , 64 ]. Similarly, another gene thymidylate synthase reported to control the expression of folate biosynthesis genes in tomato fruit was co-expressed with the SLE2F/DP 5 gene [ 65 ].…”

Unravelling the Genetic Basis of Moisture Deficit Stress Tolerance in Wheat for Seedling Vigour-Related Traits and Root Traits Using Genome-Wide Association Study

“…AX-94583923 SNP linked to coleoptile situated near the gene (TraesCS2D02G372500) on the 2D chromosome is responsible for encoding the Serine carboxypeptidase enzyme and DNA-binding pseudobarrel domain superfamily. These putative genes play a crucial role in enhancing the ability of coleoptiles and roots as well as in the development of different parts of the plant in wheat to tolerate drought conditions [61,62]. The AX-94699286 marker is associated with the gene that codes for the O-methyltransferase domain.…”