Genome-Wide Identification of Bone Metastasis-Related MicroRNAs in Lung Adenocarcinoma by High-Throughput Sequencing

Xie, Lin; Yang, Zuozhang; Li, Guoqi; Shen, Lida; Xiang, Xudong; Li, Xuefeng; Xu, Da; Xu, Lei; Chen, Yanjin; Tian, Zhao; Chen, Xin

doi:10.1371/journal.pone.0061212

Cited by 26 publications

(16 citation statements)

References 32 publications

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“…A and B). We then removed genes with extremely low FPKM (FPKM < 0.5) values in both the T and C groups on that basis . Therefore, 22 upregulated and 73 downregulated DEGs were included after filtering (Table ).…”

Section: Resultsmentioning

confidence: 99%

Transcriptome analysis in primary colorectal cancer tissues from patients with and without liver metastases using next‐generation sequencing

Wang

Zhang

et al. 2017

Cancer Medicine

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Colorectal cancer (CRC) is the third most common cancer worldwide and liver metastases are the leading cause of death in patients with CRC. In this study, we performed next‐generation sequencing profiling on primary colorectal tumor tissues obtained from three CRC patients with liver metastases and three CRC patients without liver metastases to identify differentially expressed genes (DEGs) that might be responsible for the metastases process. After filtering 2690 DEGs, comprising 996 upregulated and 1694 downregulated RNAs, 22 upregulated and 73 downregulated DEGs were identified. Gene ontology (GO) and pathway analyses were performed to determine the underlying mechanisms. Single‐organism process (biological process), cell (cellular component), and binding (molecular function) were the most related terms in the GO analysis. We selected the top 13 upregulated and top 12 downregulated genes by fold change to verify their differential expression using quantitative real‐time reverse transcription PCR (qRT‐PCR) and immunohistochemistry (IHC). The validation showed that three most significantly upregulated DEGs were HOXD10,UGT2A3, and SLC13A2, whereas the five most significantly downregulated DEGs were SPP1,CXCL8,MMP3,OSM, and CXCL6, respectively. These aberrantly expressed genes may play pivotal roles in promoting or inhibiting metastases. Further studies are required to determine the functions of DEGs to promote the diagnosis of metastases and provide novel chemotherapy targets.

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Section: Resultsmentioning

confidence: 99%

Transcriptome analysis in primary colorectal cancer tissues from patients with and without liver metastases using next‐generation sequencing

Wang

Zhang

et al. 2017

Cancer Medicine

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“…Recently, the correlation of dysregulation of miRNAs with LAD is increasingly reported. By high-throughput sequencing combined with differential expression analysis, Xie' et al identified that 7 microRNAs were down-regulated and 21 microRNAs were up-regulated in LAD with bone metastasis [10]. By performing microRNA expression profiles of whole blood in lung adenocarcinoma, Patnaik and his colleagues showed that four microRNAs (miR-190b, miR-630, miR-942, and miR-1284) were the most frequent constituents of the classifiers generated during the analyses, suggesting that whole blood microRNA expression profiles can be used to distinguish lung cancer cases from clinically relevant controls [11].…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-409-3p Functions as a Tumor Suppressor in Human Lung Adenocarcinoma by Targeting c-Met

Wan

Zhu²,

Xu³

et al. 2014

Cell Physiol Biochem

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Background/Aims: Dysregulation of microRNAs is correlated with tumor development. The aim of this study is to investigate the clinicopathologic and prognostic significance of microRNA (miR)-409-3p and its tumor suppressor roles in lung adenocarcinoma (LAD). Methods: Quantitative real-time PCR (qRT-PCR) was performed to detect miR-409-3p expression in LAD tissues and corresponding noncancerous tissues. Additionally, the correlations of miR-409-3p expression with clinicopathologic factors and prognosis of patients were statistically analyzed. Next, we investigated whether miR-409-3p could function as a tumor suppressor in LAD cells via regulation of Akt signaling by targeting receptor tyrosine kinase (c-Met). Results: MiR-409-3p was significantly downregulated in LAD tissues compared with corresponding noncancerous tissues. Low miR-409-3p expression was observed to be significantly correlated with poorer tumor differentiation, advanced pTNM stage and higher incidence of lymph node metastasis. Multivariate Cox regression analyses showed that miR-409-3p expression was an independent prognostic factor for LAD patients. Functional analyses indicated that miR-409-3p could inhibit growth, induce apoptosis, reduce migration and invasion in LAD cells via inactivation of Akt signaling by targeting c-Met. Conclusions: MiR-409-3p was an independent prognostic factor and functioned as a tumor suppressor in LAD via regulation of Akt signaling by targeting c-Met.

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“…In a recent study, Xie and colleagues constructed and compared two sRNA libraries from the blood samples of patients with lung adenocarcinoma with and without bone metastasis [70]. The data showed that seven miRNAs were downregulated and 21 miRNAs were upregulated in lung adenocarcinoma with bone metastasis.…”

Section: • • Mirnas For the Detection Of Bone Metastasis From Breast mentioning

confidence: 99%

The Role of miRNAs in Bone Metastasis and their Significance in the Detection of Bone Metastasis: A Review of the Published Data

Huang

Ouyang

2015

Future Oncol.

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The preferential metastasis of cancer cells to bone disrupts the process of bone remodeling and results in serious complications. Although bone imaging techniques are well established for bone metastasis diagnosis, they still have limits. Recently, small noncoding RNA molecules, called miRNAs, have become the subject of interest in many molecular pathways in relation to bone metastasis. Furthermore, studies have demonstrated the ability to distinguish normal from cancerous cells and metastatic bone tumor origin based on miRNA profiles. Here, we summarize the data on mechanisms of osteolytic and osteoblastic bone metastases supporting the involvement of miRNA changes in the bone metastatic evolution. We also focus on the available evidence regarding current clinical studies of miRNA expression in the detection of bone metastases.

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Genome-Wide Identification of Bone Metastasis-Related MicroRNAs in Lung Adenocarcinoma by High-Throughput Sequencing

Cited by 26 publications

References 32 publications

Transcriptome analysis in primary colorectal cancer tissues from patients with and without liver metastases using next‐generation sequencing

Transcriptome analysis in primary colorectal cancer tissues from patients with and without liver metastases using next‐generation sequencing

MicroRNA-409-3p Functions as a Tumor Suppressor in Human Lung Adenocarcinoma by Targeting c-Met

The Role of miRNAs in Bone Metastasis and their Significance in the Detection of Bone Metastasis: A Review of the Published Data

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