2020
DOI: 10.1038/s41598-020-62287-2
|View full text |Cite
|
Sign up to set email alerts
|

Genome-wide identification of essential genes in Mycobacterium intracellulare by transposon sequencing — Implication for metabolic remodeling

Abstract: The global incidence of the human nontuberculous mycobacteria (NTM) disease is rapidly increasing. However, knowledge of gene essentiality under optimal growth conditions and conditions relevant to the natural ecology of NTM, such as hypoxia, is lacking. In this study, we utilized transposon sequencing to comprehensively identify genes essential for growth in Mycobacterium intracellulare. Of 5126 genes of M. intracellulare ATCC13950, 506 genes were identified as essential genes, of which 280 and 158 genes were… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

2
15
0
1

Year Published

2021
2021
2024
2024

Publication Types

Select...
5
3

Relationship

3
5

Authors

Journals

citations
Cited by 14 publications
(18 citation statements)
references
References 70 publications
(112 reference statements)
2
15
0
1
Order By: Relevance
“…In contrast to M. tuberculosis , biological experiments using deletion mutants have not been reported for pathogenic mycobacteria, with the exception of M. marinum that mimics M. tuberculosis as it possesses the ESX-1 secretion system [ 47 ]. We have recently modified a gene manipulation method for M. intracellulare using mycobacterial plasmids and phages developed for M. tuberculosis [ 44 ]. The next step will be to analyze the biological effects in these deletion mutants to fully elucidate the molecular mechanisms of pulmonary MAC disease.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…In contrast to M. tuberculosis , biological experiments using deletion mutants have not been reported for pathogenic mycobacteria, with the exception of M. marinum that mimics M. tuberculosis as it possesses the ESX-1 secretion system [ 47 ]. We have recently modified a gene manipulation method for M. intracellulare using mycobacterial plasmids and phages developed for M. tuberculosis [ 44 ]. The next step will be to analyze the biological effects in these deletion mutants to fully elucidate the molecular mechanisms of pulmonary MAC disease.…”
Section: Discussionmentioning
confidence: 99%
“…Identifying the mce operons as group-specific genes is an important step in elucidating the virulence factors in M. intracellulare and its related strains. In particular, the mce1 operon seems to be a promising candidate of virulence factors with evidence of its role in hypoxic biofilm formation, as revealed by the genome-wide identification of essential genes by transposon sequencing [44]. In M. yongonense, the lateral gene transfer event has been suggested in rpoB gene from a distantly related scotochromogen M. parascrofulaceum by multi-locus sequence typing [45].…”
Section: Discussionmentioning
confidence: 99%
“…viability). They are usually defined operationally in natural or specific conditions ( Karash and Kwon, 2018 ) with those genes required for basic metabolism or growth in the natural host or environment identified as essential ( Tateishi et al , 2020 ). Genes required for specific biological processes like motility, drug resistance, cell division etc.…”
Section: Introductionmentioning
confidence: 99%
“…First, this study identi ed several virulent strains of M. intracellulare but did not identify the speci c virulence factors involved in disease progression. Although the development of gene manipulation technology has been delayed, we have established a transposon mutagenesis system 43 with which we are constructing deletion mutants of M. intracellulare. Furthermore, the genomes of the clinical strains used in this study have been completely sequenced 16 .…”
Section: Discussionmentioning
confidence: 99%