Genome-wide identification of FAR gene family and functional analysis of NlFAR10 during embryogenesis in the brown planthopper Nilaparvata lugens

Fan, Xiaolong; Zhang, Wenqing

doi:10.1016/j.ijbiomac.2022.11.075

Cited by 1 publication

(4 citation statements)

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“…FAR is ubiquitously found in various organisms, relying on NAD(P)H to catalyse FA precursors containing different carbon chains into fatty alcohols (Fan & Zhang, 2022), and fatty alcohols serve as crucial precursors in insects for synthesising pheromones, wax esters and stratum CHCs (Li et al, 2020). As a member of the FAR family, NlFAR7 is particularly relevant to cuticular lipid synthesis.…”

Section: Discussionmentioning

confidence: 99%

“…ds GFP was used as a negative control. Microinjection of N. lugens with dsRNA was conducted according to a previous method (Fan & Zhang, 2022; Pan et al, 2018). Each group was injected with one hundred fourth‐instar (day 1) nymphs.…”

Section: Methodsmentioning

confidence: 99%

“…In addition, dsPsFAR-treated Phenacoccus solenopsis exhibited decreased cuticular waxy layer and CHC content, while desiccation and deltamethrin treatments increased their mortality (Tong et al, 2022). Furthermore, the deletion of NlFAR10 in N. lugens has been reported to affect lipid metabolism and purine metabolism during embryogenesis, resulting in inhibited embryogenesis and low hatching rate (Fan & Zhang, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, ds PsFAR ‐treated Phenacoccus solenopsis exhibited decreased cuticular waxy layer and CHC content, while desiccation and deltamethrin treatments increased their mortality (Tong et al, 2022). Furthermore, the deletion of NlFAR10 in N. lugens has been reported to affect lipid metabolism and purine metabolism during embryogenesis, resulting in inhibited embryogenesis and low hatching rate (Fan & Zhang, 2022). Our previous studies have shown that in N. lugens , RNA interference (RNAi) knockdown against 20 ELO and 17 FAR genes to find that four ELO s and two FAR s ( NlFAR7 and NlFAR9 ) can lead to a significant decrease of CHC content (Li, Chen, Wang, Moussian, & Zhang, 2019; Li, Chen, Wang, & Zhang, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Silencing NlFAR7 destroyed the pore canals and related structures of the brown planthopper

Cui,

Guo,

Zhang

et al. 2024

Insect Molecular Biology

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Fatty acyl‐CoA reductase (FAR) is one of the key enzymes, which catalyses the conversion of fatty acyl‐CoA to the corresponding alcohols. Among the FAR family members in the brown planthopper (Nilaparvata lugens), NlFAR7 plays a pivotal role in both the synthesis of cuticular hydrocarbons and the waterproofing of the cuticle. However, the precise mechanism by which NlFAR7 influences the formation of the cuticle structure in N. lugens remains unclear. Therefore, this paper aims to investigate the impact of NlFAR7 through RNA interference, transmission electron microscope, focused ion beam scanning electron microscopy (FIB‐SEM) and lipidomics analysis. FIB‐SEM is employed to reconstruct the three‐dimensional (3D) architecture of the pore canals and related cuticle structures in N. lugens subjected to dsNlFAR7 and dsGFP treatments, enabling a comprehensive assessment of changes in the cuticle structures. The results reveal a reduction in the thickness of the cuticle and disruptions in the spiral structure of pore canals, accompanied by widened base and middle diameters. Furthermore, the lipidomics comparison analysis between dsNlFAR7‐ and dsGFP‐treated N. lugens demonstrated that there were 25 metabolites involved in cuticular lipid layer synthesis, including 7 triacylglycerols (TGs), 5 phosphatidylcholines (PCs), 3 phosphatidylethanolamines (PEs) and 2 diacylglycerols (DGs) decreased, and 4 triacylglycerols (TGs) and 4 PEs increased. In conclusion, silencing NlFAR7 disrupts the synthesis of overall lipids and destroys the cuticular pore canals and related structures, thereby disrupting the secretion of cuticular lipids, thus affecting the cuticular waterproofing of N. lugens. These findings give significant attention with reference to further biochemical researches on the substrate specificity of FAR protein, and the molecular regulation mechanisms during N. lugens life cycle.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%