Genome-Wide Identification of GDSL-Type Esterase/Lipase Gene Family in Dasypyrum villosum L. Reveals That DvGELP53 Is Related to BSMV Infection

Abstract

GDSL-type esterase/lipase proteins (GELPs) characterized by a conserved GDSL motif at their N-terminus belong to the lipid hydrolysis enzyme superfamily. In plants, GELPs play an important role in plant growth, development and stress response. The studies of the identification and characterization of the GELP gene family in Triticeae have not been reported. In this study, 193 DvGELPs were identified in Dasypyrum villosum and classified into 11 groups (clade A–K) by means of phylogenetic analysis. Most DvGELPs contain only one GDSL domain, only four DvGELPs contain other domains besides the GDSL domain. Gene structure analysis indicated 35.2% DvGELP genes have four introns and five exons. In the promoter regions of the identified DvGELPs, we detected 4502 putative cis-elements, which were associated with plant hormones, plant growth, environmental stress and light responsiveness. Expression profiling revealed 36, 44 and 17 DvGELPs were highly expressed in the spike, the root and the grain, respectively. Further investigation of a root-specific expressing GELP, DvGELP53, indicated it was induced by a variety of biotic and abiotic stresses. The knockdown of DvGELP53 inhibited long-distance movement of BSMV in the tissue of D. villosum. This research provides a genome-wide glimpse of the D. villosum GELP genes and hints at the participation of DvGELP53 in the interaction between virus and plants.

The binding pocket properties were fundamental to functional diversification of the GDSL-type esterases/lipases gene family in cotton

“…A large number of GELP genes are present in plants. Different numbers (65–194) of GDSL genes have been reported in different plants ( Chepyshko et al., 2012 ; Dong et al., 2016 ; Lai et al., 2017 ; Cao et al., 2018 ; An et al., 2019 ; Li et al., 2019 ; Ni et al., 2020 ; Su et al., 2020 ; Lv et al., 2021 ; Ren et al., 2021 ; Zhang et al., 2021 ; Jiao et al., 2022 ; Sun et al., 2022 ; Zhang et al., 2022 ). Therefore, it was not difficult to find that allotetraploid cotton harboured a larger number of GELP genes.…”

“…In recent years, an increasing number of GELP families have been identified and characterized in various plant species. For instance, a large family of GELPs have been identified and characterized in Arabidopsis ( Lai et al., 2017 ), Glycine max ( Su et al., 2020 ), Vitis vinifera L. ( Ni et al., 2020 ), Citrullus lanatus ( Ren et al., 2021 ), Sedum alfredii ( Li et al., 2019 ), Brassica rapa ( Dong et al., 2016 ), Rosaceae ( Cao et al., 2018 ), Oryza sativa ( Chepyshko et al., 2012 ), Nicotiana tabacum ( Lv et al., 2021 ), Dasypyrum villosum ( Zhang et al., 2021 ), Pyrus spp. ( Zhang et al., 2022 ), Solanum lycopersicum ( Sun et al., 2022 ), Sorghum bicolor , Picea sitchensis , Populus trichocarpa , and Physcomitrella patens ( Volokita et al., 2011 ).…”

Genome-wide exploration of the GDSL-type esterase/lipase gene family in rapeseed reveals several BnGELP proteins active during early seedling development

“…Multiomics technologies, including genomics, transcriptomics, proteomics and metabolomics, provide opportunities to systematically explore all the genes belonging to a specified gene family within a species ( Zhan et al., 2022 ). Numerous GELP family members have been identified in different plant species ( Chepyshko et al., 2012 ; Dong et al., 2016 ; Lai et al., 2017 ; Su et al., 2020 ; Lv et al., 2021 ; Wang et al., 2021 ; Zhang et al., 2021 ; Cenci et al., 2022 ; Jiao et al., 2022 ; Song et al., 2022 ; Zhan et al., 2022 ). Recently, a high-quality genome assembly and numerous transcriptome data sets have been released for B. napus cultivar ZS11 ( Chen et al., 2021 ; Liu et al., 2021 ), making identification and expression analysis of genes such as the BnGELPs more feasible.…”

“…A crucial first step in transferring the knowledge acquired for model species to non-model species is homology identification. To date, the GELP family has been identified in diverse plant species including many with fully sequenced genomes, such as Arabidopsis thaliana (105 members) , Dasypyrum villosum (193) , Sedum alfredii (80), Nicotiana tabacum (159), Glycine max (194) , Oryza sativa (114) , Dendrobium catenatum (52), Carya illinoensis (87) , Zea mays (103) , Vitis vinifera (83), Brassica rapa (121), and six species in the Rosaceae family ( Pyrus bretschneideri , Malus domestica , Prunus mume , Prunus persica , Fragaria vesca and Prunus avium ; for a total of 597 GELP s) ( Chepyshko et al., 2012 ; Dong et al., 2016 ; Lai et al., 2017 ; Ding et al., 2019b ; Su et al., 2020 ; Lv et al., 2021 ; Wang et al., 2021 ; Zhang et al., 2021 ; Cenci et al., 2022 ; Jiao et al., 2022 ; Shen et al., 2022 ). Some GELP genes have been cloned and functionally characterized in B. napus , such as BnLIP2 ( Ling et al., 2006 ), Bn SCE3 ( Clauss et al., 2008 ), BnGDSL1 ( Ding et al., 2019a ), and BnSFAR1 - 5 ( Karunarathna et al., 2020 ).…”

Genome-Wide Classification and Phylogenetic Analyses of the GDSL-Type Esterase/Lipase (GELP) Family in Flowering Plants

“…The possible reactivity of different substrates has been suggested for GELP enzymes [ 5 ]. Interest in the GELP family is growing and a number of crop-specific transcriptomic studies have been conducted in several plants, including Brassica rapa [ 6 ], Sedum alfredii [ 7 ], soybean [ 8 ], Dasypyrum villosum [ 9 ], tomato [ 10 ], and wheat [ 11 ], generating datasets at the genome scale.…”