Genome-wide identification of genes involved in raffinose metabolism in Maize

Zhou, Meiliang; Zhang, Qian; Zhou, Ming; Sun, Zhanmin; Zhu, Xuemei; Shao, Ji-Rong; Tang, Yixiong; Wu, Yihan

doi:10.1093/glycob/cws121

Cited by 46 publications

(42 citation statements)

References 38 publications

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“…1). In addition, XvGolS, ArGolS1 and BnGolS also emerge APSAA characteristics31, BhGolS1 demonstrates PPTA characteristics32 and ZmGolS37 only has a proline residue in its C terminus, but other ZmGolS genes demonstrate APSAA characteristics33. The differences in the termini are likely connected with GolS function.…”

Section: Discussionmentioning

confidence: 98%

Molecular cloning and expression of an encoding galactinol synthase gene (AnGolS1) in seedling of Ammopiptanthus nanus

Liu

Zhang

Chen

et al. 2016

Sci Rep

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Based on the galactinol synthase (AnGolS1) fragment sequence from a cold-induced Suppression Subtractive Hybridization (SSH) library derived from Ammopiptanthus nanus (A. nanus) seedlings, AnGolS1 mRNA (including the 5′ UTR and 3′ UTR) (GenBank accession number: GU942748) was isolated and characterized by rapid amplification of cDNA ends polymerase chain reaction (RACE–PCR). A substrate reaction test revealed that AnGolS1 possessed galactinol synthase activity in vitro and could potentially be an early-responsive gene. Furthermore, quantitative real-time PCR (qRT-PCR) indicated that AnGolS1 was responded to cold, salts and drought stresses, however, significantly up-regulated in all origans by low temperatures, especially in plant stems. In addition, the hybridization signals in the fascicular cambium were strongest in all cells under low temperature. Thus, we propose that AnGolS1 plays critical roles in A. nanus low-temperature stress resistance and that fascicular cambium cells could be involved in AnGolS1 mRNA transcription, galactinol transportation and coordination under low-temperature stress.

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Section: Discussionmentioning

confidence: 98%

Molecular cloning and expression of an encoding galactinol synthase gene (AnGolS1) in seedling of Ammopiptanthus nanus

Liu

Zhang

Chen

et al. 2016

Sci Rep

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“…accumulation of myoinositol and raffinose synthesize higher degrees of RFOs such as stachyose. The pathways of RFO metabolism reconstructed from genomic information revealed that most of the key enzymes are encoded by multiple gene members with different expression patterns (Zhou et al, 2012), indicating the operation and importance of RFO metabolism in maize. RFOs can also serve as mobile and storage carbon sources with advantages in osmolytic and mobile flexibility over Suc and starch, respectively (Van den Ende, 2013).…”

Section: Myoinositol As a Potential Metabolic Marker For Breeding Of mentioning

confidence: 99%

Metabolite profiles of maize leaves in drought, heat and combined stress field trials reveal the relationship between metabolism and grain yield

et al. 2015

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The development of abiotic stress-resistant cultivars is of premium importance for the agriculture of developing countries. Further progress in maize (Zea mays) performance under stresses is expected by combining marker-assisted breeding with metabolite markers. In order to dissect metabolic responses and to identify promising metabolite marker candidates, metabolite profiles of maize leaves were analyzed and compared with grain yield in field trials. Plants were grown under well-watered conditions (control) or exposed to drought, heat, and both stresses simultaneously. Trials were conducted in 2010 and 2011 using 10 tropical hybrids selected to exhibit diverse abiotic stress tolerance. Drought stress evoked the accumulation of many amino acids, including isoleucine, valine, threonine, and 4-aminobutanoate, which has been commonly reported in both field and greenhouse experiments in many plant species. Two photorespiratory amino acids, glycine and serine, and myoinositol also accumulated under drought. The combination of drought and heat evoked relatively few specific responses, and most of the metabolic changes were predictable from the sum of the responses to individual stresses. Statistical analysis revealed significant correlation between levels of glycine and myoinositol and grain yield under drought. Levels of myoinositol in control conditions were also related to grain yield under drought. Furthermore, multiple linear regression models very well explained the variation of grain yield via the combination of several metabolites. These results indicate the importance of photorespiration and raffinose family oligosaccharide metabolism in grain yield under drought and suggest single or multiple metabolites as potential metabolic markers for the breeding of abiotic stress-tolerant maize.

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“…Our previously published data show that there are more than one PsGolS and PsRS genes ; however, only one of each of them have been cloned so far. In other plant species, abiotic stresses up-regulated the expression of different GolS and RS genes (Taji et al 2002;Wang et al 2012a;Zhou et al 2012Zhou et al , 2014, indicating the complexity of galactinol and RFOs regulation under stress conditions. The induction of the expression of GolS genes in response to water stress was demonstrated in vegetative tissues of different plant species.…”

Section: Expression Of Psgols Psrs and Pssts Genes And Activity Of Ementioning

confidence: 99%

Osmotic stress induces genes, enzymes and accumulation of galactinol, raffinose and stachyose in seedlings of pea (Pisum sativum L.)

et al. 2015

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The objective of the present study was to recognize the molecular background of the accumulation of raffinose family oligosaccharides (RFOs) in pea (Pisum sativum L.) seedlings under osmotic stress conditions. The exposure of 5-day-old pea seedlings to osmotic stress for 48 h created by immersing roots in PEG8000 solution (-1.5 MPa) induced synthesis of galactinol and RFOs (raffinose and stachyose) in the epicotyl and root tissues, but not in cotyledons. After 24 h of recovery, galactinol completely disappeared, raffinose decreased fourfold and stachyose decreased twofold in roots, but increased in epicotyls. The temporary accumulation of RFOs resulted from a dramatic increase in the enzymatic activity and changes in expression of galactinol synthase (PsGolS), raffinose synthase (PsRS) and stachyose synthase (PsSTS) genes. PsGolS was induced by osmotic stress in both epicotyls as well as in roots. PsRS and PsSTS were induced only in epicotyls, but repressed or remained unaffected in roots, respectively. During recovery, the expression and activity of PsGolS, PsRS and PsSTS dramatically decreased. The expression of PsGolS gene, that level of mRNA transcript significantly decreased during recovery and whose promoter region was identified to contain some stress-related regulating elements, seems to play a crucial role in the biosynthesis of RFOs under osmotic stress. Possible signals that may trigger the induction of expression of PsGolS, PsRS and PsSTS genes and accumulation of RFOs in pea seedlings are discussed.

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Genome-wide identification of genes involved in raffinose metabolism in Maize

Cited by 46 publications

References 38 publications

Molecular cloning and expression of an encoding galactinol synthase gene (AnGolS1) in seedling of Ammopiptanthus nanus

Molecular cloning and expression of an encoding galactinol synthase gene (AnGolS1) in seedling of Ammopiptanthus nanus

Metabolite profiles of maize leaves in drought, heat and combined stress field trials reveal the relationship between metabolism and grain yield

Osmotic stress induces genes, enzymes and accumulation of galactinol, raffinose and stachyose in seedlings of pea (Pisum sativum L.)

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