Genome-wide identification, phylogenetic analysis, and expression profiling of polyamine synthesis gene family members in tomato

Liu, Taibo; Huang, Binbin; Chen, Lin; Xian, Zhiqiang; Song, Shiwei; Chen, Riyuan; Hao, Yanwei

doi:10.1016/j.gene.2018.03.084

Cited by 35 publications

(33 citation statements)

References 43 publications

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“…Most of the earlier studies have focused primarily on PA biosynthesis while PAs catabolism/back conversion is less studied in tomato fruit ripening [48] and grape ripening [75]. Recently, PA biosynthesis [56] and PAO mediated catabolism [57] under stress has been reported but changes in PAs levels were not determined in these investigations. In conclusion, whereas PAO4-like and CuAO4 were up regulated in heat stress, PAO2 and CuAO4-like were upregulated under cold stress, contrasting their roles in the two stresses studied here.…”

Section: Discussionmentioning

confidence: 99%

Polyamines and Their Biosynthesis/Catabolism Genes Are Differentially Modulated in Response to Heat Versus Cold Stress in Tomato Leaves (Solanum lycopersicum L.)

Fatima¹,

Handa²,

Mattoo

2020

Cells

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Polyamines (PAs) regulate growth in plants and modulate the whole plant life cycle. They have been associated with different abiotic and biotic stresses, but little is known about the molecular regulation involved. We quantified gene expression of PA anabolic and catabolic pathway enzymes in tomato (Solanum lycopersicum cv. Ailsa Craig) leaves under heat versus cold stress. These include arginase 1 and 2, arginine decarboxylase 1 and 2, agmatine iminohydrolase/deiminase 1, N-carbamoyl putrescine amidase, two ornithine decarboxylases, three S-adenosylmethionine decarboxylases, two spermidine synthases; spermine synthase; flavin-dependent polyamine oxidases (SlPAO4-like and SlPAO2) and copper dependent amine oxidases (SlCuAO and SlCuAO-like). The spatiotemporal transcript abundances using qRT-PCR revealed presence of their transcripts in all tissues examined, with higher transcript levels observed for SAMDC1, SAMDC2 and ADC2 in most tissues. Cellular levels of free and conjugated forms of putrescine and spermidine were found to decline during heat stress while they increased in response to cold stress, revealing their differential responses. Transcript levels of ARG2, SPDS2, and PAO4-like increased in response to both heat and cold stresses. However, transcript levels of ARG1/2, AIH1, CPA, SPDS1 and CuAO4 increased in response to heat while those of ARG2, ADC1,2, ODC1, SAMDC1,2,3, PAO2 and CuPAO4-like increased in response to cold stress, respectively. Transcripts of ADC1,2, ODC1,2, and SPMS declined in response to heat stress while ODC2 transcripts declined under cold stress. These results show differential expression of PA metabolism genes under heat and cold stresses with more impairment clearly seen under heat stress. We interpret these results to indicate a more pronounced role of PAs in cold stress acclimation compared to that under heat stress in tomato leaves.

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Section: Discussionmentioning

confidence: 99%

Polyamines and Their Biosynthesis/Catabolism Genes Are Differentially Modulated in Response to Heat Versus Cold Stress in Tomato Leaves (Solanum lycopersicum L.)

Fatima¹,

Handa²,

Mattoo

2020

Cells

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“…Over the past several decades, substantial progress has been made in characterizing the PA roles related to fruit development and ripening in tomato (Mutschler, 1984;Dibble et al, 1988;Saftner and Baldiet, 1990;Mehta et al, 2002;Tassoni et al, 2006;Mattoo et al, 2006Mattoo et al, , 2007Gapper et al, 2013;Tsaniklidis et al, 2016;Sharma et al, 2017;Hao et al, 2018;Liu et al, 2018). Notably, a recent report described a positive role for Spm and Spd, especially Spm, in the regulation of strawberry fruit ripening because it manipulates the expression of FaSAMDC, which is important for the biosynthesis of Spd and Spm (Guo et al, 2018).…”

Section: Polyamines Play Essential Roles In Strawberry Fruit Ripeningmentioning

confidence: 99%

“…There has been considerable progress in the functional characterization of ethylene in climacteric fruits, such as tomato (Solanum lycopersicum; Alexander & Grierson, 2002;Liu, Pirrello, Chervin, Roustan, & Bouzayen, 2015), and the elucidation of the roles of ABA in non-climacteric fruits, including strawberry (Fragaria ananassa; Rose & Shen, 2014). Notably, there is increasing evidence that polyamines (PAs) substantially affect fruit ripening (Kushad, Yelenosky, & Knight, 1988;Dibble, Davies, & Mutschler, 1988;Rodriguez, López, & Chaves, 1999;Lester, 2000; de Dios, Matilla, & Gallardo, 2006;Parra-Lobato et al, 2011;Koushesh, Arzani, & Barzegar, 2012;Gupta, Pal, & Rajam, 2013;Jiang et al, 2015;Fortes, Teixeira, & Agudelo-Romero, 2015;Simpson et al, 2017;Guo et al, 2018;Liu et al, 2018). Although PAs are important for strawberry fruit ripening, the molecular mechanisms underlying PA accumulation remain unclear.…”

Section: Introductionmentioning

confidence: 99%

“…Since early reports on a role of PAs in fleshy fruits (Cohen, Arad, Heimer, & Mizrahi, 1982;Winer, Vinkler, & Apelbaum, 1984), over the past years, much progress has been made toward understanding PA roles in fruit development and ripening (Kushad et al, 1988;Dibble et al, 1988;Rodriguez et al, 1999;Lester, 2000;Bregoli et al, 2002;de Dios et al, 2006;Parra-Lobato & Gomez, 2011;Koushesh et al, 2012;Kitashiba, Hao, Honda, & Moriguchi, 2005;Jiang et al, 2015;Fortes et al, 2015;Simpson et al, 2017;Guo et al, 2018). Taken together, the effects of PAs vary with the composition and content of polyamines, fruit types, and developmental stages, to a certain extent, the ratios of (Spd + Spm)/Put control ripening of climacteric fruits (Guo et al, 2018;Liu et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…The known fruit ripening-related tomato mutants have enabled researchers to use tomato as a model species for studying the effects of PA on ripening (Dibble et al, 1988;Gapper, McQuinn, & Giovannoni, 2013;Hao et al, 2018;Liu et al, 2018;Mattoo et al, 2006Mattoo et al, , 2007Mehta et al, 2002;Mutschler, 1984;Saftner & Baldi, 1990;Sharma, Pareek, Sagar, Valero, & Serrano, 2017;Tassoni, Watkins, & Davies, 2006;Tsaniklidis et al, 2016). An increase in Put contents may prolong the tomato fruit ripening process (Dibble et al, 1988;Saftner & Baldi, 1990;Tassoni et al, 2006;Tsaniklidis et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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FaPAO5 regulates Spm/Spd levels as a signaling during strawberry fruit ripening

Bai

et al. 2020

Plant Direct

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Polyamines are important for non-climacteric fruit ripening according to an analysis of the model plant strawberry. However, the molecular mechanism underlying the polyamine accumulation during ripening has not been fully elucidated. In this study, an examination of our proteome data related to strawberry fruit ripening revealed a putative polyamine oxidase 5, FaPAO5, which was localized in the cytoplasm and nucleus. Additionally, FaPAO5 expression levels as well as the abundance of the encoded protein continually decreased during ripening. Inhibiting FaPAO5 expression by RNAi promoted Spd, Spm, and ABA accumulation while inhibited H 2 O 2 production, which ultimately enhanced ripening as evidenced by the ripening-related events and corresponding gene expression changes. The opposite effects were observed in FaPAO5-overexpressing transgenic fruits. Analyses of the binding affinity and enzymatic activity of FaPAO5 with Spm, Spd, and Put uncovered a special role for FaPAO5 in the terminal catabolism of Spm and Spd, with a K d of 0.21 and 0.29 µM, respectively. Moreover, FaPAO5 expression was inhibited by ABA and promoted by Spd and Spm. Furthermore, the RNA-seq analysis of RNAi and control fruits via differentially expressed genes (DEGs) indicated the six most enriched pathways among the differentially expressed genes were related to sugar, abscisic acid, ethylene, auxin, gibberellin, and Ca 2+ . Among four putative PAO genes in the strawberry genome,only FaPAO5 was confirmed to influence fruit ripening. In conclusion, FaPAO5 is a negative regulator of strawberry fruit ripening and modulates Spm/Spd levels as a signaling event, in which ABA plays a central role.

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Synthesis of putrescine‐imprinted double‐layer nanofiber membrane by electrospinning for the selective recognition of putrescine

Ying

Huang

2020

J of Applied Polymer Sci

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In this work, we have developed a double‐layer nanofiber membrane (NFM) with high selectivity based on molecular imprinting technique by electrospinning methods and applied it in recognizing putrescine. Poly(vinyl alcohol) (PVA) was used as spinning solution, 1,4‐butylene glycol as the imitate template, and ninhydrin as chromogenic agent. The adsorption of the putrescine is corresponded to purple‐color stains on the NFM, which is digitizing intuitively by the ImageJ software. By quantifying the gray value of the stains on the NFM, its imprinting efficiency (IE) is estimated. The adsorption performance of the double‐layer molecular imprinted NFM was studied in detail, showing higher adsorption capacity (71.912 mg mL−1), shorter adsorption equilibrium time of 40 min and higher selectivity to putrescine than our previous works. Furthermore, the material showed remarkable selectivity for putrescine with respect to other three kinds of aliphatic amine in similar molecular structure. The selectivity coefficient (α) of putrescine against cadaverine, spermine, and spermidine are calculated to be 1.734, 2.224, and 2.576, respectively. In addition, since PVA is a water‐soluble material, the NFM is not reusable. © 2020 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48932.

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Genome-wide identification, phylogenetic analysis, and expression profiling of polyamine synthesis gene family members in tomato

Cited by 35 publications

References 43 publications

Polyamines and Their Biosynthesis/Catabolism Genes Are Differentially Modulated in Response to Heat Versus Cold Stress in Tomato Leaves (Solanum lycopersicum L.)

Polyamines and Their Biosynthesis/Catabolism Genes Are Differentially Modulated in Response to Heat Versus Cold Stress in Tomato Leaves (Solanum lycopersicum L.)

FaPAO5 regulates Spm/Spd levels as a signaling during strawberry fruit ripening

Synthesis of putrescine‐imprinted double‐layer nanofiber membrane by electrospinning for the selective recognition of putrescine

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