Genome-wide inactivation of porcine endogenous retroviruses (PERVs)

Yang, Luhan; Niu, Dong; George, Haydy; Lesha, Emal; Grishin, Dennis; Aach, John; Shrock, Ellen; Xu, Weihong; Poci, Jürgen; Cortazio, Rebeca; Wilkinson, Robert A.; Fishman, Jay A.; Church, George M.

doi:10.1126/science.aad1191

Cited by 531 publications

(418 citation statements)

References 25 publications

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“…This has enabled, for example, the creation of liver tumors by direct injection of CRISPR/Cas9 to delete Pten and p53 in the same cells [19]. This method has been used to disrupt as many as 62 endogenous retrovirus loci, which pose potential risks in clinical application, in order to generate pigs that are safe to use in pig-to-human xenotransplantation [20].…”

Section: Application Of Crispr/cas9mentioning

confidence: 99%

Genome editing: A breakthrough in life science and medicine [Review]

Hatada

Horii

2016

Endocr J

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Section: Application Of Crispr/cas9mentioning

confidence: 99%

Genome editing: A breakthrough in life science and medicine [Review]

Hatada

Horii

2016

Endocr J

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“…In our lifetime, we may see foods or pets customized and marketed to suit individual's whims. Researchers have already proposed using CRISPR to modify pigs to develop human-compatible organs (Yang et al 2015). CRISPR could also be used to genetically modify domestic pets to be more appealing to humans.…”

Section: Revolutionary Social Impactmentioning

confidence: 99%

CRISPR as a driving force: the Model T of biotechnology

Mariscal

Petropanagos

2016

Monash Bioeth. Rev.

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The CRISPR system for gene editing can break, repair, and replace targeted sections of DNA. Although CRISPR gene editing has important therapeutic potential, it raises several ethical concerns. Some bioethicists worry CRISPR is a prelude to a dystopian future, while others maintain it should not be feared because it is analogous to past biotechnologies. In the scientific literature, CRISPR is often discussed as a revolutionary technology. In this paper we unpack the framing of CRISPR as a revolutionary technology and contrast it with framing it as a valuethreatening biotechnology or business-as-usual. By drawing on a comparison between CRISPR and the Ford Model T, we argue CRISPR is revolutionary as a product, process, and as a force for social change. This characterization of CRISPR offers important conceptual clarity to the existing debates surrounding CRISPR. In particular, conceptualizing CRISPR as a revolutionary technology structures regulatory goals with respect to this new technology. Revolutionary technologies have characteristic patterns of implementation, entrenchment, and social impact. As such, early identification of technologies as revolutionary may help construct more nuanced and effective ethical frameworks for public policy.

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“…Additionally, it will The humanization of mice and larger animals such as cattle, pigs, and cats will be streamlined by efficient orthologous gene(s) KO using genome editing, and the generation of various humanized Tc animal models may promote drug development and therapy [102][103][104]. Once HACs/MACs with gene(s) of interest are constructed, they can be transferred to various mammalian cells and maintained independently without integration into the host genome.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%