Genome-wide inventory of metal homeostasis-related gene products including a functional phytochelatin synthase in the hypogeous mycorrhizal fungus Tuber melanosporum

Bolchi, Angelo; Ruotolo, Roberta; Marchini, Gessica; Vurro, Emanuela; Toppi, Luigi Sanità di; Kohler, Annegret; Tisserant, Émilie; Martin, Francis; Ottonello, Simone

doi:10.1016/j.fgb.2010.11.003

Cited by 51 publications

(38 citation statements)

References 86 publications

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“…The available annotated T. melanosporum genome offers novel insights into key biological functions. Genome-wide transcriptome studies have unraveled several developmental and metabolic pathways regulated by symbiosis and fruiting body formation, as well as environmental factors (Amicucci et al, 2011;Bolchi et al, 2011;Ceccaroli et al, 2011;Montanini et al, 2011;Zampieri et al, 2011). Changes in gene expression during hyphal growth (Amicucci et al, 2011) and upon cold stress (Zampieri et al, 2011), as well as genes involved in metal homeostasis are discussed in the present issue of Fungal, Genetics and Biology.…”

Section: Ascocarp Growth and Maturationmentioning

confidence: 99%

On the road to understanding truffles in the underground

Kües

Martin

2011

Fungal Genetics and Biology

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Section: Ascocarp Growth and Maturationmentioning

confidence: 99%

On the road to understanding truffles in the underground

Kües

Martin

2011

Fungal Genetics and Biology

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“…The genome sequence of the T. melanosporum black truffle fruiting body suggests that truffles possess all the genes needed to synthesize the key constituents of the truffle aroma (Martin et al 2010), and the Post-Genomics Era has been open to truffle research (Balestrini and Mello 2014;Kües and Martin 2011). Recently, the genomewide transcriptome studies have unraveled several developmental and metabolic pathways regulated by symbiosis and fruiting body formation, as well as environmental factors (Amicucci et al 2011;Bolchi et al 2011;Ceccaroli et al 2011;Montanini et al 2011;Zampieri et al 2011). However, the genes and enzymes involved in the biosynthesis of aroma active compounds, umami amino acids and 5′-nucleotides, and phytosterol are still unclear.…”

Section: Conclusion and Future Prospectsmentioning

confidence: 99%

Current progress on truffle submerged fermentation: a promising alternative to its fruiting bodies

Tang

Liu

2015

Appl Microbiol Biotechnol

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Truffle (Tuber spp.), also known as "underground gold," is popular in various cuisines because of its unique and characteristic aroma. Currently, truffle fruiting bodies are mostly obtained from nature and semi-artificial cultivation. However, the former source is scarce, and the latter is time-consuming, usually taking 4 to 12 years before harvest of the fruiting body. The truffle submerged fermentation process was first developed in Tang's lab as an alternative to its fruiting bodies. To the best of our knowledge, most reports of truffle submerged fermentation come from Tang's group. This review examines the current state of the truffle submerged fermentation process. First, the strategy to optimize the truffle submerged fermentation process is summarized; the final conditions yielded not only the highest reported truffle biomass but also the highest production of extracellular and intracellular polysaccharides. Second, the comparison of metabolites produced by truffle fermentation and fruiting bodies is presented, and the former were superior to the latter. Third, metabolites (i.e., volatile organic compounds, equivalent umami concentration, and sterol) derived from truffle fermentation could be regulated by fermentation process optimization. These findings indicated that submerged fermentation of truffles can be used for commercial production of biomass and metabolites as a promising alternative to generating its fruiting bodies in bioreactor.

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“…As with L. bicolor , the sequenced genome facilitated the identification and analysis of many gene families of interest to symbiosis studies, including CAZymes (induced during mycorrhiza formation, apparently to force a path between the root cells for the mycelium), lipases, multicopper oxidases, an invertase (unlike L. bicolor ), other carbohydrate metabolism enzymes (Ceccaroli et al, 2011), metal detoxification genes (Bolchi et al, 2011), and cell wall metabolism enzymes (Balestrini et al, 2012; Sillo et al, 2013). As this genome was also the first sequenced for the Pezizomycetes, it also prompted investigation in another fungal clade of genes of general mycological interest, such as cytoskeleton components that determine hyphal morphology (Amicucci et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Expanding genomics of mycorrhizal symbiosis

et al. 2014

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The mycorrhizal symbiosis between soil fungi and plant roots is a ubiquitous mutualism that plays key roles in plant nutrition, soil health, and carbon cycling. The symbiosis evolved repeatedly and independently as multiple morphotypes [e.g., arbuscular mycorrhizae (AM), ectomycorrhizal (ECM)] in multiple fungal clades (e.g., phyla Glomeromycota, Ascomycota, Basidiomycota). The accessibility and cultivability of many mycorrhizal partners make them ideal models for symbiosis studies. Alongside molecular, physiological, and ecological investigations, sequencing led to the first three mycorrhizal fungal genomes, representing two morphotypes and three phyla. The genome of the ECM basidiomycete Laccaria bicolor showed that the mycorrhizal lifestyle can evolve through loss of plant cell wall-degrading enzymes (PCWDEs) and expansion of lineage-specific gene families such as short secreted protein (SSP) effectors. The genome of the ECM ascomycete Tuber melanosporum showed that the ECM type can evolve without expansion of families as in Laccaria, and thus a different set of symbiosis genes. The genome of the AM glomeromycete Rhizophagus irregularis showed that despite enormous phylogenetic distance and morphological difference from the other two fungi, symbiosis can involve similar solutions as symbiosis-induced SSPs and loss of PCWDEs. The three genomes provide a solid base for addressing fundamental questions about the nature and role of a vital mutualism.

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Genome-wide inventory of metal homeostasis-related gene products including a functional phytochelatin synthase in the hypogeous mycorrhizal fungus Tuber melanosporum

Cited by 51 publications

References 86 publications

On the road to understanding truffles in the underground

On the road to understanding truffles in the underground

Current progress on truffle submerged fermentation: a promising alternative to its fruiting bodies

Expanding genomics of mycorrhizal symbiosis

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