Genome-wide investigation of bHLH genes and expression analysis under different biotic and abiotic stresses in Helianthus annuus L.

Li, Juanjuan; Li, Xin; Han, Peng; Liu, Hui; Gong, Jianchuan; Zhou, Weijun; Shi, Bixian; Liu, Ake; Xu, Ling

doi:10.1016/j.ijbiomac.2021.08.072

Cited by 45 publications

(32 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the bHLH TFs in A. coerulea have not been reported. In this study, we identified 120 bHLHs with complete domains in A. coerulea, which is less than that in Helianthus annuus (183), A. thaliana (162), and rice (167) (Heim et al, 2003; Sun et al, 2015; Li et al, 2021b), and more than that in sweet cherry (66) and Nicotiana tabacum (100) (Bano and Patel, 2021; Shen et al, 2021). Given that the genome size of A. coerulea (∼300M) is larger than A. thaliana (∼125M) and smaller than rice (∼466M) and N. tabacum (∼4.5G), the difference in the number of identified bHLH genes among these species is related to genome size as suggested by Li et al (2021).…”

Section: Discussionmentioning

confidence: 93%

“…Sequence analysis revealed that each putative AqbHLH protein contains four conserved regions including a basic region, a loop region and two helixes ( Figure 1 ). The four conserved regions were highly conserved in the majority of the AqbHLH genes, as well as in A. thaliana , sunflower and spine grapes (Bano and Patel, 2021; Li et al, 2021b). The bHLH domain is important for DNA binding and dimerization (Murre et al, 1989), which contained Leu-23, Pro-28, Tyr-47, Leu-51 and Leu-58 with conservation of more than 90% in A. coerulea .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Genome-wide identification and expression analysis of bHLH transcription factors reveal their putative regulatory effects on petal nectar spur development in Aquilegia

Huang

Zhang

2022

Preprint

View full text Add to dashboard Cite

The basic helix-loop-helix (bHLH) transcription factors (TFs) control a diversity of organ morphogenesis involved in cell division and cell expansion processes. The development of petal nectar spur, which plays important roles in plant-pollinator interaction and adaptive radiation, comprised cell division and cell expansion phases in Aquilegia. Here, we conducted a genome-wide identification of the bHLH gene family in Aquilegia to determine the characteristics and the expression profiles of this gene family during the development of petal nectar spur. A total of 120 AqbHLH proteins were identified from the Aquilegia coerulea genome. The phylogenetic tree showed that AqbHLH members were divided into 15 subfamilies, among which S7 and S8 subfamilies occurred marked expansion. Nineteen residues with conservation of more than 50% were found in the four conserved regions. The publicly RNA-Seq data and qRT-PCR results showed that AqbHLH027, AqbHLH083, AqbHLH046, and AqbHLH092 would be associated with the development of petal nectar spur by regulating cell division and cell cycle in phase I. While AqbHLH036 might participate the spur cell elongation and cell expansion in phase Ⅱ. This study provides useful insights for further probing on the function of AqbHLH TFs in the regulation of petal nectar spur development.

show abstract

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Genome-wide identification and expression analysis of bHLH transcription factors reveal their putative regulatory effects on petal nectar spur development in Aquilegia

Huang

Zhang

2022

Preprint

View full text Add to dashboard Cite

show abstract

“…In addition, the transcription factor family has contributed to resisting adverse environmental factors in plants, such as drought resistance, salt tolerance, cold tolerance, plant iron deficiency stress, and so on (Toledo-Ortiz et al, 2003). A great many bHLH genes have been distinguished in the plant kingdom, including A. thaliana (162) (Pires and Dolan, 2010), Hibiscus hamabo (162) (Ni et al, 2021), Helianthus annuus (183) (Li et al, 2021), Capsicum annuum (107) , Sorghum bicolor (174) (Fan et al, 2021), Osmanthus fragrans (206) (Li et al, 2020), Camellia sinensis (134) , Juglans regia (102) (Zhao et al, 2021), Pyrus bretschneideri (197) (Dong et al, 2021). The number of bHLH transcription factors changed widely among various plants.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Low-Temperature Expression Analysis of bHLH Genes in Prunus mume

Ding

et al. 2021

Front. Genet.

View full text Add to dashboard Cite

Prunus mume is an illustrious ornamental woody plant with colorful flowers, delicate fragrances, and graceful tree forms. Low temperature limits its geographical distribution. The basic helix-loop-helix (bHLH) proteins exist in most eukaryotes as a transcription factor superfamily, which play a crucial role in metabolism, physiology, development, and response to various stresses of higher organisms. However, the characteristics of the bHLH gene family and low-temperature response remain unknown in P. mume. In the present study, we distinguished 95 PmbHLH genes in the P. mume whole-genome and analyzed their features. PmbHLHs were divided into 23 subfamilies and one orphan by phylogenetic analysis. Similar gene structures and conserved motifs appeared in the same subfamily. These genes were situated in eight chromosomes and scaffolds. Gene duplication events performed a close relationship to P. mume, P. persica, and P. avium. Tandem duplications probably promoted the expansion of PmbHLHs. According to predicted binding activities, the PmbHLHs were defined as the Non-DNA-binding proteins and DNA-binding proteins. Furthermore, PmbHLHs exhibited tissue-specific and low-temperature induced expression patterns. By analyzing transcriptome data, 10 PmbHLHs which are responsive to low-temperature stress were selected. The qRT-PCR results showed that the ten PmbHLH genes could respond to low-temperature stress at different degrees. There were differences in multiple variations among different varieties. This study provides a basis to research the evolution and low-temperature tolerance of PmbHLHs, and might enhance breeding programs of P. mume by improving low-temperature tolerance.

show abstract

Section: Introductionmentioning

confidence: 99%

“…bHLH TFs constitute the second major type of regulatory protein in plants [ 19 ]. These TFs have a specific bHLH domain, and they are involved in plant growth and development of plants and the response to abiotic stress [ 19 ]. MYC2 TFs are types of bHLH TFs and are important regulators of the jasmonic acid (JA) signaling pathway [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

The Caucasian Clover Gene TaMYC2 Responds to Abiotic Stress and Improves Tolerance by Increasing the Activity of Antioxidant Enzymes

Zhao

Yang

Jiang

et al. 2022

Genes

View full text Add to dashboard Cite

Abiotic stress affects metabolic processes in plants and restricts plant growth and development. In this experiment, Caucasian clover (Trifolium ambiguum M. Bieb.) was used as a material, and the CDS of TaMYC2, which is involved in regulating the response to abiotic stress, was cloned. The CDS of TaMYC2 was 726 bp in length and encoded 241 amino acids. The protein encoded by TaMYC2 was determined to be unstable, be highly hydrophilic, and contain 23 phosphorylation sites. Subcellular localization results showed that TaMYC2 was localized in the nucleus. TaMYC2 responded to salt, alkali, cold, and drought stress and could be induced by IAA, GA3, and MeJA. By analyzing the gene expression and antioxidant enzyme activity in plants before and after stress, we found that drought and cold stress could induce the expression of TaMYC2 and increase the antioxidant enzyme activity. TaMYC2 could also induce the expression of ROS scavenging-related and stress-responsive genes and increase the activity of antioxidant enzymes, thus improving the ability of plants to resist stress. The results of this experiment provide references for subsequent in-depth exploration of both the function of TaMYC2 in and the molecular mechanism underlying the resistance of Caucasian clover.

show abstract

Genome-wide investigation of bHLH genes and expression analysis under different biotic and abiotic stresses in Helianthus annuus L.

Cited by 45 publications

References 66 publications

Genome-wide identification and expression analysis of bHLH transcription factors reveal their putative regulatory effects on petal nectar spur development in Aquilegia

Genome-wide identification and expression analysis of bHLH transcription factors reveal their putative regulatory effects on petal nectar spur development in Aquilegia

Genome-Wide Identification and Low-Temperature Expression Analysis of bHLH Genes in Prunus mume

The Caucasian Clover Gene TaMYC2 Responds to Abiotic Stress and Improves Tolerance by Increasing the Activity of Antioxidant Enzymes

Contact Info

Product

Resources

About