Genome-Wide Investigation of G6PDH Gene in Strawberry: Evolution and Expression Analysis during Development and Stress

Lei, Diya; Lin, Yuanxiu; Luo, Mengwen; Zhao, Bing; Tang, Huaping; Zou, Xuan; Yao, Wantian; Zhang, Yunting; Wang, Yan; Li, Mengyao; Chen, Qing; Luo, Yunbo; Wang, Xiaorong; Tang, Haoru; Zhang, Yong

doi:10.3390/ijms23094728

Cited by 5 publications

(5 citation statements)

References 37 publications

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“…In winter wheat, TaG6PDH (Triticum aestivum G6PDH) expression was upregulated under cold stress and exogenous ABA application, suggesting that TaG6PDH positively responds to cold stress and ABA [129]. Similarly, FaG6PDH positively regulated cold tolerance in strawberry [130], and in silico bioinformatics analysis of 19 FaG6PDH promoters revealed the presence of at least one stress-responsive cis-acting element [131]. An early stress response would involve the OPPP, which represents a true metabolic sensor during the response to various stresses.…”

Section: Drought and Heatmentioning

confidence: 99%

Glucose-6-Phosphate Dehydrogenases: The Hidden Players of Plant Physiology

Jiang

Wang

Nicolas

et al. 2022

IJMS

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Glucose-6-phosphate dehydrogenase (G6PDH) catalyzes a metabolic hub between glycolysis and the pentose phosphate pathway (PPP), which is the oxidation of glucose-6-phosphate (G6P) to 6-phosphogluconolactone concomitantly with the production of nicotinamide adenine dinucleotide phosphate (NADPH), a reducing power. It is considered to be the rate-limiting step that governs carbon flow through the oxidative pentose phosphate pathway (OPPP). The OPPP is the main supplier of reductant (NADPH) for several “reducing” biosynthetic reactions. Although it is involved in multiple physiological processes, current knowledge on its exact role and regulation is still piecemeal. The present review provides a concise and comprehensive picture of the diversity of plant G6PDHs and their role in seed germination, nitrogen assimilation, plant branching, and plant response to abiotic stress. This work will help define future research directions to improve our knowledge of G6PDHs in plant physiology and to integrate this hidden player in plant performance.

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Section: Drought and Heatmentioning

confidence: 99%

Glucose-6-Phosphate Dehydrogenases: The Hidden Players of Plant Physiology

Jiang

Wang

Nicolas

et al. 2022

IJMS

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“…Under abiotic stress, it has been reported that the organic carbon of plant species results in a shift from EMP to PPP, in a percentage even higher than 50% [33,34]. PPP enhances NADPH production [35,36], promoting the cells' metabolic activity, including the carbonand nitrogen-metabolizing enzymes (Figure 1b). The shift to the PPP was also conducive to nitrate reduction and the formation of RuBP, which promoted the regenerative capacity of RuBP in the Calvin-Benson cycle.…”

Section: Glucose Metabolism (Emp and Ppp) And Growth In Bn And Ov Pla...mentioning

confidence: 99%

Quantification of Glucose Metabolism and Nitrogen Utilization in Two Brassicaceae Species under Bicarbonate and Variable Ammonium Soil Conditions

Xia,

Wu,

Xiang

et al. 2023

Plants

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In karst habitats under drought conditions, high bicarbonate (high pH), and an abundant nitrate soil environment, bicarbonate regulates the glycolysis (EMP) and pentose phosphate pathways (PPP), which distribute ATP and NADPH, affecting nitrate (NO3−) and ammonium (NH4+) utilization in plants. However, the relationship between EMP PPP and NO3−, and NH4+ utilization and their responses to bicarbonate and variable ammonium still remains elusive. In this study, we used Brassica napus (Bn, a non-karst-adaptable plant) and Orychophragmus violaceus (Ov, a karst-adaptable plant) as plant materials, employed a bidirectional nitrogen-isotope-tracing method, and performed the quantification of the contribution of EMP and PPP. We found that bicarbonate and ammonium inhibited glucose metabolism and nitrogen utilization in Bn under simulated karst habitats. On the other hand, it resulted in a shift from EMP to PPP to promote ammonium utilization in Ov under high ammonium stress in karst habitats. Compared with Bn, bicarbonate promoted glucose metabolism and nitrogen utilization in Ov at low ammonium levels, leading to an increase in photosynthesis, the PPP, carbon and nitrogen metabolizing enzyme activities, nitrate/ammonium utilization, and total inorganic nitrogen assimilation capacity. Moreover, bicarbonate significantly reduced the growth inhibition of Ov by high ammonium, resulting in an improved PPP, RCRUBP, and ammonium utilization to maintain growth. Quantifying the relationships between EMP, PPP, NO3−, and NH4+ utilization can aid the accurate analysis of carbon and nitrogen use efficiency changes in plant species. Therefore, it provides a new prospect to optimize the nitrate/ammonium utilization in plants and further reveals the differential responses of inorganic carbon and nitrogen (C-N) metabolism to bicarbonate and variable ammonium in karst habitats.

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“…The number of G6PDH genes changes significantly among plant species. Thus, 19 G6PDH genes in strawberry (Fragaria × ananassa) [18], 9 in soybean (Glycine max) [35], 6 in Arabidopsis thaliana [8], 5 in rice (Oryza sativa), Arabidopsis tauchii, tomato (Solanum lycopersicum) [27], and maize (Zea mays) [27], and 4 G6PDH genes in rubber tree (Hevea brasiliensis) [36] and einkorn wheat (Triticum urartu) [37] have been reported. Therefore, the four CaG6PDH genes identified in this work indicate that pepper is among the species with the lowest number of G6PDH genes.…”

Section: The Pepper Genome Contains Four Cag6pdh Genes Which Are All ...mentioning

confidence: 99%

“…Maize plants enclose five genes and, among them, the ZmG6PDH4 gene length is almost 18 kb with 9 exons and two large introns [27]. Strawberries have 19 G6PDH genes with 10 to 15 exons, but the largest gene which corresponds to FaG6PD14 has less than 7 kb [18]. All these examples with many introns/exons with different lengths indicate the great diversity of the G6PDH gene structures in higher plants.…”

Section: The Pepper Genome Contains Four Cag6pdh Genes Which Are All ...mentioning

confidence: 99%

“…Different studies have correlated the G6PDH activity in plants with physiological processes such as germination and development [16][17][18][19][20], but also with the mechanisms of response to numerous environmental stresses [21,22] including salinity [23][24][25], water stress [22,23], mechanical wounding, arsenic, chromium, aluminum [24], herbicides [25], low temperature [26,27], potassium deficiency or pathogens [28,29].…”

Section: Introductionmentioning

confidence: 99%

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In Silico RNAseq and Biochemical Analyses of Glucose-6-Phosphate Dehydrogenase (G6PDH) from Sweet Pepper Fruits: Involvement of Nitric Oxide (NO) in Ripening and Modulation

Muñoz-Vargas,

González-Gordo,

Taboada

et al. 2023

Plants

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Pepper (Capsicum annuum L.) fruit is a horticultural product consumed worldwide which has great nutritional and economic relevance. Besides the phenotypical changes that pepper fruit undergo during ripening, there are many associated modifications at transcriptomic, proteomic, biochemical, and metabolic levels. Nitric oxide (NO) is a recognized signal molecule that can exert regulatory functions in diverse plant processes including fruit ripening, but the relevance of NADPH as a fingerprinting of the crop physiology including ripening has also been proposed. Glucose-6-phosphate dehydrogenase (G6PDH) is the first and rate-limiting enzyme of the oxidative phase of the pentose phosphate pathway (oxiPPP) with the capacity to generate NADPH. Thus far, the available information on G6PDH and other NADPH-generating enzymatic systems in pepper plants, and their expression during the ripening of sweet pepper fruit, is very scarce. Therefore, an analysis at the transcriptomic, molecular and functional levels of the G6PDH system has been accomplished in this work for the first time. Based on a data-mining approach to the pepper genome and fruit transcriptome (RNA-seq), four G6PDH genes were identified in pepper plants and designated CaG6PDH1 to CaG6PDH4, with all of them also being expressed in fruits. While CaG6PDH1 encodes a cytosolic isozyme, the other genes code for plastid isozymes. The time-course expression analysis of these CaG6PDH genes during different fruit ripening stages, including green immature (G), breaking point (BP), and red ripe (R), showed that they were differentially modulated. Thus, while CaG6PDH2 and CaG6PDH4 were upregulated at ripening, CaG6PDH1 was downregulated, and CaG6PDH3 was slightly affected. Exogenous treatment of fruits with NO gas triggered the downregulation of CaG6PDH2, whereas the other genes were positively regulated. In-gel analysis using non-denaturing PAGE of a 50–75% ammonium-sulfate-enriched protein fraction from pepper fruits allowed for identifying two isozymes designated CaG6PDH I and CaG6PDH II, according to their electrophoretic mobility. In order to test the potential modulation of such pepper G6PDH isozymes, in vitro analyses of green pepper fruit samples in the presence of different compounds including NO donors (S-nitrosoglutathione and nitrosocysteine), peroxynitrite (ONOO−), a hydrogen sulfide (H2S) donor (NaHS, sodium hydrosulfide), and reducing agents such as reduced glutathione (GSH) and L-cysteine (L-Cys) were assayed. While peroxynitrite and the reducing compounds provoked a partial inhibition of one or both isoenzymes, NaHS exerted 100% inhibition of the two CaG6PDHs. Taken together these data provide the first data on the modulation of CaG6PDHs at gene and activity levels which occur in pepper fruit during ripening and after NO post-harvest treatment. As a consequence, this phenomenon may influence the NADPH availability for the redox homeostasis of the fruit and balance its active nitro-oxidative metabolism throughout the ripening process.

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Genome-Wide Investigation of G6PDH Gene in Strawberry: Evolution and Expression Analysis during Development and Stress

Cited by 5 publications

References 37 publications

Glucose-6-Phosphate Dehydrogenases: The Hidden Players of Plant Physiology

Glucose-6-Phosphate Dehydrogenases: The Hidden Players of Plant Physiology

Quantification of Glucose Metabolism and Nitrogen Utilization in Two Brassicaceae Species under Bicarbonate and Variable Ammonium Soil Conditions

In Silico RNAseq and Biochemical Analyses of Glucose-6-Phosphate Dehydrogenase (G6PDH) from Sweet Pepper Fruits: Involvement of Nitric Oxide (NO) in Ripening and Modulation

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