Genome wide responses of murine lungs to dietary α-tocopherol

Abstract

Abstractα-tocopherol (α-T) may affect biological processes by modulating mRNA concentrations. This study screened the responses of ~15,000 lung mRNAs to dietary α-T in mice. The lung was chosen as the target organ because it is subjected to cyclical variations in oxidant and inflammatory stressors and α-T has been implicated in their modulations. The analysis identified ~400 mRNAs sensitive to α-T status of lungs determined by dietary α-T. The female lung transcriptome appears to be more sensitive to the α-T status than that of the male lungs. Here, we focus on the induction of 13 cytoskeleton genes by dietary α-T because they were similarly induced in the male and the female lungs. Their inductions were confirmed by quantitative-real-time-polymerase chain reaction (qRT-PCR). Immunohistochemical analyses of three of the encoded proteins suggest that they are expressed in lung vasculature and alveolar regions. The data suggest that the lung α-T status may modulate cytoarchitecture of lungs.

Tocopherol transfer protein deficiency modifies nuclear receptor transcriptional networks in lungs: Modulation by cigarette smoke in vivo

“…Dietary AT deficiency also affected the expression of testes genome (Barella et al, 2004b;Rota et al, 2004) and that of the liver (Barella et al, 2004a) and lungs (Oommen et al, 2007). The latter study identified a coordinated regulation of a cluster of 13 cytoskeleton genes that appear to be regulated by serum response factor, a transcription factor important in the development of myocardium and skeletal muscle (Miano, 2003).…”

Novel Transcriptional Activities of Vitamin E: Inhibition of Cholesterol Biosynthesis

“…Recently, a number of groups utilized cDNA micro-array approaches to determine the effect of vitamin E on mRNA expression profiles. Gohil et al (16,(21)(22)(23) compared the gene expression profiles in brains and livers from TTP −/− (vitamin E-deficient) mice with those from wild-type mice and reported pronounced differences (2-to 20-fold) in the levels of multiple transcripts that play important roles in myriad biological functions. Similarly, Barella and colleagues compared the expression profile of vitamin Edepleted rats with those obtained from normal animals (17)(18)(19)56).…”

“…Moreover, αTOH was shown to regulate the expression levels of several mRNAs and proteins, such as collagen α1 (9), the scavenger receptors SR-BI (10) and CD36 (11,12), α-tropomyosin (13), the nuclear receptor PPARγ (14), and the adhesion molecule VCAM-I (15). These anecdotal observations were further supported by several studies that documented genomic responses to vitamin E using expression-profiling § To whom correspondence should be addressed at: School of Medicine, Case Western Reserve University, WG48, 2109 Adelbert Rd., Cleveland, [16][17][18][19][20][21][22][23]. However, interpretation of such analyses is complicated by the known genomic responses to changes in cellular redox status (24).…”

“…approaches (16)(17)(18)(19)(20)(21)(22)(23). However, interpretation of such analyses is complicated by the known genomic responses to changes in cellular redox status (24).…”

Impact of aging vs. estrogen loss on cardiac gene expression: estrogen replacement and inflammation

“…This approach allows repetitive analysis of all groups by the array in a group representative and cost-effective manner, given the four groups and 24 RNA samples. This approach has been recognized as a practical approach to analysis of multiple groups and has been used effectively by other investigators (2,28,33,35). The doublestrand cDNA was purified and served as a template in the subsequent in vitro transcription (IVT) reaction for complementary RNA (cRNA) amplification and biotin labeling (IVT Labeling Kit, Affymetrix, P/N 900449).…”