Genome-Wide Selective Signatures Reveal Candidate Genes Associated with Hair Follicle Development and Wool Shedding in Sheep

Lei, Zhihui; Sun, Weibo; Guo, Tingting; Li, Jianye; Zhu, Shijia; Lu, Zengkui; Qiao, Guoyan; Han, Mei Lan K.; Zhao, Hongchang; Yang, Bo‐Yi; Zhang, Liping; Liu, Jianbin; Yuan, Chao; Yuan, Yongna

doi:10.3390/genes12121924

Cited by 14 publications

(6 citation statements)

References 49 publications

(60 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Gene expression is regulated by noncoding RNAs, including miRNAs. MiRNAs, which are endogenously expressed in mammals [32][33][34], are 18-22-nucleotide (nt), small noncoding RNAs that are evolutionarily conserved and negatively regulate the expression of target mRNAs mainly by repressing translation or, in some cases, by cleaving mRNA transcripts [35,36]. miRNAs play a key role in many biological processes by regulating gene expression at the posttranscriptional level.…”

Section: Introductionmentioning

confidence: 99%

Integrated analysis of miRNAs and mRNA profiling reveals the potential roles of miRNAs in sheep hair follicle development

Huang

Zhao

et al. 2022

BMC Genomics

View full text Add to dashboard Cite

Background Merino sheep exhibit high wool production and excellent wool quality. The fleece of Merino sheep is predominantly composed of wool fibers grown from hair follicles (HFs). The HF is a complex biological system involved in a dynamic process governed by gene regulation, and gene expression is regulated by microRNAs (miRNAs). miRNA inhibits posttranscriptional gene expression by specifically binding to target messenger RNA (mRNA) and plays an important role in regulating gene expression, the cell cycle and biological development sequences. The purpose of this study was to examine mRNA and miRNA binding to identify key miRNAs and target genes related to HF development. This will provide new and important insights into fundamental mechanisms that regulate cellular activity and cell fate decisions within and outside of the skin. Results We analyzed miRNA data in skin tissues collected from 18 Merino sheep on four embryonic days (E65, E85, E105 and E135) and two postnatal days (D7 and D30) and identified 87 differentially expressed miRNAs (DE-miRNAs). These six stages were further divided into two longer developmental stages based on heatmap cluster analysis, and the results showed that DE-mRNAs in Stage A were closely related to HF morphogenesis. A coanalysis of Stage A DE-mRNAs and DE-miRNAs revealed that 9 DE-miRNAs and 17 DE-mRNAs presented targeting relationships in Stage A. We found that miR-23b and miR-133 could target and regulate ACVR1B and WNT10A. In dermal fibroblasts, the overexpression of miR-133 significantly reduced the mRNA and protein expression levels of ACVR1B. The overexpression of miR-23b significantly reduced the mRNA and protein expression levels of WNT10A. Conclusion This study provides a new reference for understanding the molecular basis of HF development and lays a foundation for further improving sheep HF breeding. miRNAs and target genes related to hair follicular development were found, which provided a theoretical basis for molecular breeding for the culture of fine-wool sheep.

show abstract

Section: Introductionmentioning

confidence: 99%

Integrated analysis of miRNAs and mRNA profiling reveals the potential roles of miRNAs in sheep hair follicle development

Huang

Zhao

et al. 2022

BMC Genomics

View full text Add to dashboard Cite

show abstract

“…Knockdown of CD80 in mouse has been shown to result in a high susceptibility to infection, leading to disease phenotypes similar to those observed in human alopecia areata [25]. PRX , involving in cell proliferation, plays an important role in HF development and wool shedding [26]. The alterations in the expression of these genes in the study suggest that HFSCARC is an important player in the HF cycle.…”

Section: Discussionmentioning

confidence: 66%

Role of HFSCARC in hair follicle stem cell proliferation and differentiation in cashmere goat

Wang,

Zhang,

Guo

et al. 2024

Animal Research and One Health

View full text Add to dashboard Cite

As a model of regenerative medicine, hair follicle stem cell (HFSC) plays a determining role in the hair cycle. Emerging evidences showed that long noncoding RNAs regulated the biological function of HFSC. In this current study, we found that lncRNA‐000552, standing for “goat secondary HFSC Associated SYNE3 Regulator of HF Cycle” (HFSCARC) expressed higher in anagen than that in telogen of cashmere goat. Through experiments involving nucleocytoplasmic separation and RNA‐FISH, we determined that HFSCARC was primarily located in the nucleus of HFSC. To understand the function of HFSCARC, the study performed various assays, including crystal violet staining, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide, EdU, and flow cytometry analysis, which collectively revealed that HFSCARC inhibited HFSC proliferation. Additionally, HFSCARC promoted the differentiation of HFSC by investigating the expression of marker genes specific to stem cells and keratinocytes. RNA sequencing analysis was conducted to investigate the global gene expression changes associated with HFSCARC expression. The results showed that HFSCARC altered the expression of genes involved in cell proliferation, hair follicle development, and regulation of bone morphogenetic proteins (BMP) signaling. Furthermore, the study revealed that HFSCARC activated the BMP signaling pathway. Intriguingly, the study found a decreased expression of SYNE3, which was a neighboring gene of HFSCARC. The altered expression of genes associated with transmethylase and demethylase further suggested that HFSCARC might play an important role in regulating the SYNE3 expression. Overall, this study provides valuable insights into the regulatory role of HFSCARC in the biological function of HFSC. These findings contribute to a better understanding of the involvement of noncoding RNAs in the regulation of hair cycle.

show abstract

“…Jin et al [23] identified SNPs with the top 5% of the XP-EHH values using SNP microarray genotyping data on 53 goats from three breeds. Lei et al [54] identified SNPs with XP-EHH <−2 using available SNP microarray genotyping data on 795 sheep from 27 breeds divided into two groups (fine-wool sheep and hair sheep). Zhang et al [55] identified SNPs with the top 1% of the XP-EHH values using sequencing data on 47 sheep from three breeds.…”

Section: Methods 3 Selection Signaturesmentioning

confidence: 99%

Genome-Wide Association Study of Fiber Diameter in Alpacas

More,

Veli,

Cruz

et al. 2023

Animals

View full text Add to dashboard Cite

The aim of this study was the identification of candidate genomic regions associated with fiber diameter in alpacas. DNA samples were collected from 1011 female Huacaya alpacas from two geographical Andean regions in Peru (Pasco and Puno), and three alpaca farms within each region. The samples were genotyped using an Affymetrix Custom Alpaca genotyping array containing 76,508 SNPs. After the quality controls, 960 samples and 51,742 SNPs were retained. Three association study methodologies were performed. The GWAS based on a linear model allowed us to identify 11 and 35 SNPs (−log10(p-values) > 4) using information on all alpacas and alpacas with extreme values of fiber diameter, respectively. The haplotype and marker analysis method allowed us to identify nine haplotypes with standardized haplotype heritability higher than six standard deviations. The selection signatures based on cross-population extended haplotype homozygosity (XP-EHH) allowed us to identify 180 SNPs with XP-EHH values greater than |3|. Four candidate regions with adjacent SNPs identified via two association methods of analysis are located on VPA6, VPA9, VPA29 and one chromosomally unassigned scaffold. This study represents the first analysis of alpaca whole genome association with fiber diameter, using a recently assembled alpaca SNP microarray.

show abstract

Genome-Wide Selective Signatures Reveal Candidate Genes Associated with Hair Follicle Development and Wool Shedding in Sheep

Cited by 14 publications

References 49 publications

Integrated analysis of miRNAs and mRNA profiling reveals the potential roles of miRNAs in sheep hair follicle development

Integrated analysis of miRNAs and mRNA profiling reveals the potential roles of miRNAs in sheep hair follicle development

Role of HFSCARC in hair follicle stem cell proliferation and differentiation in cashmere goat

Genome-Wide Association Study of Fiber Diameter in Alpacas

Contact Info

Product

Resources

About