Genome-wide sperm deoxyribonucleic acid methylation is altered in some men with abnormal chromatin packaging or poor in vitro fertilization embryogenesis

Aston, Kenneth I.; Punj, Vasu; Liu, Lihua; Carrell, Douglas T.

doi:10.1016/j.fertnstert.2011.11.008

Cited by 94 publications

(60 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, other genes have now been identified with differential gametic DNA methylation that is retained through early preimplantation development (Smallwood et al 2011, Kobayashi et al 2012. Furthermore, while genome-wide methylation analyses are limited, altered DNA methylation has been detected at both imprinted and non-imprinted genes in ART-conceived children (Katari et al 2009), in vitro cultured mouse blastocysts (Wright et al 2011), and sperm from infertile men (Houshdaran et al 2007, Pacheco et al 2011, Aston et al 2012, as well as globally in two-cell mouse embryos from superovulated mothers and sperm from men with infertility (Shi & Haaf 2002, Benchaib et al 2003. Thus, aberrant imprinted methylation may be an indicator of more global epigenetic instability arising from ARTs or underlying infertility.…”

Section: Genomic Imprinting: a Model For Epigenetic Stabilitymentioning

confidence: 99%

Genomic imprints as a model for the analysis of epigenetic stability during assisted reproductive technologies

Denomme¹,

Mann²

2012

Reproduction

112

118

View full text Add to dashboard Cite

Gamete and early embryo development are important stages when genome-scale epigenetic transitions are orchestrated. The apparent lack of remodeling of differential imprinted DNA methylation during preimplantation development has lead to the argument that epigenetic disruption by assisted reproductive technologies (ARTs) is restricted to imprinted genes. We contend that aberrant imprinted methylation arising from assisted reproduction or infertility may be an indicator of more global epigenetic instability. Here, we review the current literature on the effects of ARTs, including ovarian stimulation, in vitro oocyte maturation, oocyte cryopreservation, IVF, ICSI, embryo culture, and infertility on genomic imprinting as a model for evaluating epigenetic stability. Undoubtedly, the relationship between impaired fertility, ARTs, and epigenetic stability is unquestionably complex. What is clear is that future studies need to be directed at determining the molecular and cellular mechanisms giving rise to epigenetic errors.

show abstract

Section: Genomic Imprinting: a Model For Epigenetic Stabilitymentioning

confidence: 99%

Genomic imprints as a model for the analysis of epigenetic stability during assisted reproductive technologies

Denomme¹,

Mann²

2012

Reproduction

112

118

View full text Add to dashboard Cite

show abstract

“…Abnormal methylation of paternal DNA is also important to study because DNA methylation may be altered in the sperm of men with infertility. One study has shown that in some infertile men perturbations in methylation may contribute to abnormal embryo development (Aston et al 2012). The possibility that some aspect(s) of infertility per se contributes to altered phenotype and/or epigenotype is open for consideration and further investigation (Song et al 2015).…”

Section: Discussionmentioning

confidence: 99%

A Molecular Perspective on Procedures and Outcomes with Assisted Reproductive Technologies

Mainigi

Sapienza

Butts

et al. 2016

Cold Spring Harb Perspect Med

View full text Add to dashboard Cite

The emerging association of assisted reproductive technologies with adverse perinatal outcomes has prompted the in-depth examination of clinical and laboratory protocols and procedures and their possible effects on epigenetic regulatory mechanism(s). The application of various approaches to study epigenetic regulation to problems in reproductive medicine has the potential to identify relative risk indicators for particular conditions, diagnostic biomarkers of disease state, and prognostic indicators of outcome. Moreover, when applied genome-wide, these techniques are likely to find novel pathways of disease pathogenesis and identify new targets for intervention. The analysis of DNA methylation, histone modifications, transcription factors, enhancer binding and other chromatin proteins, DNasehypersensitivity and, micro-and other noncoding RNAs all provide overlapping and often complementary snapshots of chromatin structure and resultant "gene activity." In terms of clinical application, the predictive power and utility of epigenetic information will depend on the power of individual techniques to discriminate normal levels of interindividual variation from variation linked to a disease state. At present, quantitative analysis of DNA methylation at multiple loci seems likely to hold the greatest promise for achieving the level of precision, reproducibility, and throughput demanded in a clinical setting.

show abstract

“…Studying relation between male infertility in humans with use of immunostaining for DNA methylation in a study, if there were high global methylation levels of ejaculated sperm DNA, it corresponded to higher pregnancy rates as compared to those having methylation defeats having infertility [81]. Using Methylight staining of 36 target genes using ilumina platform showed improperly increased levels of DNA methylation of imprinted genes along with repetitive elements in poor quality semen samples [82]. According to them increased methylation occurred due to an improper erasure of methylation marks in cases of oligo-astheno-teratozoospermia (OAT), rather than from denovo methylation following epigenetic reprogramming.…”

Section: Male Infertility and Epigeneticsmentioning

confidence: 99%

An Update on Epigenetics in Mammalian Reproduction with Emphasis on Human Reproduction

G¹,

Singh²

2016

J Clin Epigenet

View full text Add to dashboard Cite

Basically epigenetic mechanisms control development and regulate gene expression in various types of cells of the organism, each carrying similar DNA sequence. In simple language, the nucleotides of DNA are letters of the complicated text and these epigenetic labels or marks are the spaces, punctuations, sentences, paragraphs and styles which give meaning to this complicated text. Here we have tried to discuss the epigenetic markers like DNA methylation at CpG dinucleotide, covalent modifications of histone proteins and details of noncoding RNA's including long ncRNA's, miRNA. Besides importance of lncRNA'S has been discussed in dosage compensation in X linked genes with role of Xi where heavy dosage of X linked genes get compensated with role of Xist and long noncoding RNAs and further the value of genome imprinting in ART and various disorders like Beckman Wiedeman Syndrome (BWS), Russell silver syndrome (RSS) is discussed with just opposing marks on similar loci on same chromosomes. Further role of environmental stressors in stresses is discussed. Role of famines in both Chinese as well as Dutch famines highlights immediate changes can affect on future generations.Environmental pollutants and medications may affect fetal epigenetic marks e.g., is in choline intake in pregnancy increased placental promoter methylation of cortisol regulation genes CRH and NR3IC=>improved stress response in children by lowering cortisol levels in H-P-A axis. Thus future of epigenetics research lies in understanding the effects of interaction of epigenetics and environment emphasis on fetal programming understanding and uncovering role of medicine and nutrition and assesses risk for adult onset disease.

show abstract

Genome-wide sperm deoxyribonucleic acid methylation is altered in some men with abnormal chromatin packaging or poor in vitro fertilization embryogenesis

Cited by 94 publications

References 47 publications

Genomic imprints as a model for the analysis of epigenetic stability during assisted reproductive technologies

Genomic imprints as a model for the analysis of epigenetic stability during assisted reproductive technologies

A Molecular Perspective on Procedures and Outcomes with Assisted Reproductive Technologies

An Update on Epigenetics in Mammalian Reproduction with Emphasis on Human Reproduction

Contact Info

Product

Resources

About