Genome-wide survey and characterization of the WRKY gene family in Populus trichocarpa

He, Hongsheng; Dong, Qing; Shao, Yuanhua; Jiang, Haiyang; Zhu, Suwen; Cheng, Beijiu; Xiang, Yan

doi:10.1007/s00299-012-1241-0

Cited by 200 publications

(213 citation statements)

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“…Since the first WRKY cDNA was cloned from sweet potato (Ipomoea batatas) (Ishiguro and Nakamura 1994), many WRKY genes have been extensively identified and characterized in various plant species (Eulgem et al 2000;Wu et al 2005;Li et al 2012;Tripathi et al 2012;Xiong et al 2013). In the Arabidopsis genome, at least 72 WRKY family members have been identified (Eulgem et al 2000), 102 in rice (Wu et al 2005), 55 in cucumber (Ling et al 2011), and 105 in poplar (He et al 2012). In addition, several WRKY-type genes were recently detected in the lineage of non-plant eukaryotes (Giardia lamblia; Dictyostelium discoideum) and green alga (Chlamydomonas reinhardtii), implying that the WRKY transcription factor family has its origin in eukaryotes and is largely amplified in plants (Zhang and Wang 2005).…”

Section: Introductionmentioning

confidence: 98%

Identification and expression analysis of WRKY family genes under biotic and abiotic stresses in Brassica rapa

et al. 2014

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WRKY proteins constitute one of the largest transcription factor families in higher plants, and they are involved in multiple biological processes such as plant development, metabolism, and responses to biotic and abiotic stresses. Genes of this family have been well documented in response to many abiotic and biotic stresses in many plant species, but not yet against Pectobacterium carotovorum subsp. carotovorum and Fusarium oxysporum f.sp. conglutinans in any of the plants. Moreover, potentiality of a specific gene may vary depending on stress conditions and genotypes. To identify stress resistance-related potential WRKY genes of Brassica rapa, we analyzed their expressions against above-mentioned pathogens and cold, salt, and drought stresses in B. rapa. Stress resistance-related functions of all Brassica rapa WRKY (BrWRKY) genes were firstly analyzed through homology study with existing biotic and abiotic stress resistance-related WRKY genes of other plant species and found a high degree of homology. We then identified all BrWRKY genes in a Br135K microarray dataset, which was created by applying low-temperature stresses to two contrasting Chinese cabbage doubled haploid (DH) lines, Chiifu and Kenshin, and selected 41 BrWRKY genes with high and differential transcript abundance levels. These selected genes were further investigated under cold, salt, and drought stresses as well as after infection with P. carotovorum subsp. carotovorum and F. oxysporum f.sp. conglutinans in B. rapa. The selected genes showed an organ-specific expression, and 22 BrWRKY genes were differentially expressed in Chiifu compared to Kenshin under cold and drought stresses. Six BrWRKY genes were more responsive in Kenshin compared to Chiffu under salt stress. In addition, eight BrWRKY genes showed differential expression after P. carotovorum subsp. carotovorum infection and five genes after F. oxysporum f.sp. conglutinans infection in B. rapa. Thus, the differentially expressed BrWRKY genes might be potential resources for molecular breeding of Brassica crops against abiotic and biotic stresses and several genes, which showed differential expressions commonly in response to several stresses, might be useful for multiple stress resistance. These findings would also be helpful in resolving the complex regulatory mechanism of WRKY genes in stress resistance and for this further functional genomics study of these potential genes in different Brassica crops is essential.

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Section: Introductionmentioning

confidence: 98%

Identification and expression analysis of WRKY family genes under biotic and abiotic stresses in Brassica rapa

et al. 2014

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“…The first identified WRKY gene, SPF1, was cloned from sweet potato (Ipomoea batatas) 20 years ago [16]. Since then, a large number of WRKY genes have been identified, including 74 from Arabidopsis thaliana [17], 103 from Oryza sativa [18], 45 from Hordeum vulgare [19], 119 from Zea mays [20], and many more from other plant species [21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis of WRKY gene family in the sesame genome and identification of the WRKY genes involved in responses to abiotic stresses

Liu

et al. 2017

BMC Plant Biol

111

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Background: Sesame (Sesamum indicum L.) is one of the world's most important oil crops. However, it is susceptible to abiotic stresses in general, and to waterlogging and drought stresses in particular. The molecular mechanisms of abiotic stress tolerance in sesame have not yet been elucidated. The WRKY domain transcription factors play significant roles in plant growth, development, and responses to stresses. However, little is known about the number, location, structure, molecular phylogenetics, and expression of the WRKY genes in sesame.

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“…Since the first WRKY gene, SPF1, was cloned from sweet potato (Ishiguro and Nakamura 1994), a large number of WRKY genes were identified and characterized from different plant species, such as tobacco (Yang et al 1999;Chen and Chen 2000), Arabidopsis (Eulgem et al 2000;Dong et al 2003), parsley (Cormack et al 2002), barley (Sun et al 2003), rice (Xie et al 2005;Ross et al 2007), coffee (Ramiro et al 2010), cucumber (Ling et al 2011), poplar (He et al 2012), soybean (Yin et al 2013), physic nut (Xiong et al 2013), brachypodium distachyon (Wen et al 2014), and grape (Guo et al 2014).…”

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The expression patterns of Cucumis sativus WRKY (CsWRKY) family under the condition of inoculation with Phytophthora melonis in disease resistant and susceptible cucumber cultivars

et al. 2015

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, T. 2015. The expression patterns of Cucumis sativus WRKY (CsWRKY) family under the condition of inoculation with Phytophthora melonis in disease resistant and susceptible cucumber cultivars. Can. J. Plant Sci. 95: 1121Á1131. Based on RNA-seq, we analyzed expression patterns of 55 CsWRKYs in the disease-resistant cultivar (JSH) and the disease-susceptible cucumber cultivar (B80) at 0 and 40 h post-inoculation with Phytophthora melonis. After inoculation with P. melonis, in JSH and B80, the number of up-regulated CsWRKY genes was both 25 with 22 commonly up-regulated genes, and there were 22 CsWRKY genes down-regulated in JSH and 23 in B80 with 18 commonly down-regulated genes, and there were eight CsWRKY genes with no or little change in transcript expression in JSH and seven in B80 with four common genes. Among the 55 CsWRKY genes, 17 genes showed significant differences (differences twofold) in the degree of change of gene transcription level between JSH and B80 from RNA-seq data analysis, and the results validated using real-time PCR showed that there were only six genes (CsWRKY2, 20, 26, 35, 44, 52) out of the 17 that exhibited significant differences (differences twofold) with four genes (CsWRKY2, 20, 26, 52) consistent with the data from RNA-seq and two genes (CsWRKY 35, 44) inconsistent with the data from RNA-seq. In addition, the six genes were strongly up-regulated after salicylic acid (SA) treatment with five genes (CsWRKY2, 20, 26, 35, 44) up to peak at 12 h and one gene (CsWRKY52) up to peak at 24 h in expression and that four CsWRKY genes (CsWRKY2, 20, 44, 52) out of six were strongly up-regulated with three genes (CsWRKY2, 20, 44) up to the highest point at 24 h and one gene (CsWRKY52) up after methyl jasmonate (MeJA) treatment. Based on the results above, we predicted CsWRKY2, 20, 26, 35, 44 and 52 may be involved in disease resistance of JSH against Phytophthora melonis by SA and (or) JA signaling pathway(s).Key words: Cucumis sativus, CsWRKY, expression patterns, Phytophthora melonis Xu, X., Wang, R., Chao, J., Lin, Y., Jin, Q., He, X., Luo, S. et Wu, T. 2015. Modes d'expression de la famille de ge`nes WRKY (CsWRKY) de Cucumis sativus apre`s inoculation de Phytophthora melonis chez les cultivars de concombre re´sistant et sensible a`la maladie. Can. J. Plant Sci. 95: 1121Á1131. Se fiant sur la se´quence de l'ARN, les auteurs ont analyse´le mode d'expression de 55 ge`nes CsWRKY chez les cultivars de concombre re´sistant (JSH) et sensible (B80) a`Phytophthora melonis (P. melonis), 0 h et 40 h apre`s inoculation du pathoge`ne. Apre`s inoculation, ils ont de´nombre´25 ge`nes CsWRKY re´gule´s en amont chez JSH et B80, dont 22 qui l'e´taient normalement, ainsi que 23 ge`nes CsWRKY re´gule´s en aval chez JSH et 23 chez B80, dont 18 l'e´tant normalement. S'y ajoutaient 8 ge`nes CsWRKY dont l'expression n'avait pas change´ou tre`s peu apre`s la transcription chez JSH, contre 7 chez B80, y compris quatre ge`nes courants. Sur les 55 ge`nes CsWRKY, 17 avaient subi un changement significatif (plus du doub...

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Genome-wide survey and characterization of the WRKY gene family in Populus trichocarpa

Abstract: This study identified 104 poplar WRKY genes and demonstrated WRKY gene hot spots on chromosome 14. Furthermore, semi-quantitative RT-PCR showed variable stress responses in subgroup III.

Cited by 200 publications

References 56 publications

Identification and expression analysis of WRKY family genes under biotic and abiotic stresses in Brassica rapa

Identification and expression analysis of WRKY family genes under biotic and abiotic stresses in Brassica rapa

Genome-wide analysis of WRKY gene family in the sesame genome and identification of the WRKY genes involved in responses to abiotic stresses

The expression patterns of Cucumis sativus WRKY (CsWRKY) family under the condition of inoculation with Phytophthora melonis in disease resistant and susceptible cucumber cultivars

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