Genome-wide transcriptional analysis of grapevine berry ripening reveals a set of genes similarly modulated during three seasons and the occurrence of an oxidative burst at vèraison

Pilati, S.; Perazzolli, Michele; Malossini, A.; Cestaro, Alessandro; Dematté, L.; Fontana, Paolo; Rì, A. Dal; Viola, Roberto; Velasco, Riccardo; Moser, C.

doi:10.1186/1471-2164-8-428

Cited by 231 publications

(259 citation statements)

References 92 publications

(115 reference statements)

Supporting

Mentioning

226

Contrasting

Unclassified

Order By: Relevance

“…Taken together, these data revealed that genes expressed during the immature green phase of development are inhibited after veraison, whereas genes related to sugar accumulation and secondary metabolism are induced. As in the atlas data set, the switch genes appear to regulate the immature-tomature phase transition by the mass downregulation of target genes rather than the activation of new pathways (Lijavetzky et al, 2006;Deluc et al, 2007;Pilati et al, 2007;Lund et al, 2008;Agudelo-Romero et al, 2013).…”

Section: The Grapevine Berry Switch Genes Mainly Encode Transcriptionmentioning

confidence: 99%

Integrated Network Analysis Identifies Fight-Club Nodes as a Class of Hubs Encompassing Key Putative Switch Genes That Induce Major Transcriptome Reprogramming during Grapevine Development

et al. 2014

View full text Add to dashboard Cite

We developed an approach that integrates different network-based methods to analyze the correlation network arising from large-scale gene expression data. By studying grapevine (Vitis vinifera) and tomato (Solanum lycopersicum) gene expression atlases and a grapevine berry transcriptomic data set during the transition from immature to mature growth, we identified a category named "fight-club hubs" characterized by a marked negative correlation with the expression profiles of neighboring genes in the network. A special subset named "switch genes" was identified, with the additional property of many significant negative correlations outside their own group in the network. Switch genes are involved in multiple processes and include transcription factors that may be considered master regulators of the previously reported transcriptome remodeling that marks the developmental shift from immature to mature growth. All switch genes, expressed at low levels in vegetative/green tissues, showed a significant increase in mature/woody organs, suggesting a potential regulatory role during the developmental transition. Finally, our analysis of tomato gene expression data sets showed that wild-type switch genes are downregulated in ripening-deficient mutants. The identification of known master regulators of tomato fruit maturation suggests our method is suitable for the detection of key regulators of organ development in different fleshy fruit crops.

show abstract

Section: The Grapevine Berry Switch Genes Mainly Encode Transcriptionmentioning

confidence: 99%

Integrated Network Analysis Identifies Fight-Club Nodes as a Class of Hubs Encompassing Key Putative Switch Genes That Induce Major Transcriptome Reprogramming during Grapevine Development

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Recent studies have demonstrated that there is a large increase in hydrogen peroxide production postveraison (Pilati et al, 2007), and aquaporins have been shown to facilitate hydrogen peroxide transport across membranes (Bienert et al, 2007). In addition, the ripening process in general is associated with large increases in sugar transport and accumulation, changes in cell wall metabolism, and changes in turgor, all of which are certainly impacted by aquaporins and their modulation of membrane water permeability.…”

Section: Aquaporin Gene Expression and Changes In R Hmentioning

confidence: 99%

Vascular Function in Grape Berries across Development and Its Relevance to Apparent Hydraulic Isolation

et al. 2009

View full text Add to dashboard Cite

During the latter stages of development in fleshy fruit, water flow through the xylem declines markedly and the requirements of transpiration and further expansion are fulfilled primarily by the phloem. We evaluated the hypothesis that cessation of water transport through the xylem results from disruption or occlusion of pedicel and berry xylem conduits (hydraulic isolation). Xylem hydraulic resistance (R h ) was measured in developing fruit of grape (Vitis vinifera 'Chardonnay') 20 to 100 d after anthesis (DAA) and compared with observations of xylem anatomy by light and cryo-scanning electron microscopy and expression of six plasma membrane intrinsic protein (PIP) aquaporin genes (VvPIP1;1, VvPIP1;2, VvPIP1;3, VvPIP2;1, VvPIP2;2, VvPIP2;3). There was a significant increase in whole berry R h and receptacle R h in the latter stages of ripening (80-100 DAA), which was associated with deposition of gels or solutes in many receptacle xylem conduits. Peaks in the expression of some aquaporin isoforms corresponded to lower whole berry R h 60 to 80 DAA, and the increase in R h beginning at 80 DAA correlated with decreases in the expression of the two most predominantly expressed PIP genes. Although significant, the increase in berry R h was not great enough, and occurred too late in development, to explain the decline in xylem flow that occurs at 60 to 75 DAA. The evidence suggests that the fruit is not hydraulically isolated from the parent plant by xylem occlusion but, rather, is "hydraulically buffered" by water delivered via the phloem.

show abstract

“…The respective fruits are themselves named "climacteric." As grapevine is not amongst them, it came somewhat as a surprise when Pilati et al (2007) reported an oxidative burst in cv. "Pinot Noir" that began at veraison and was characterized by rapid accumulation of H 2 O 2 and by the modulation of many ROS scavenging enzymes, previously thought not to be up-regulated in this species.…”

Section: Oxidative Burstmentioning

confidence: 99%

“…Indole-3-acetic acid (IAA) content is high from anthesis to veraison, then declines to very low levels at maturation (Conde et al, 2007). Amidase (AMI1), responsible for the synthesis of IAA from indole-3-acetamide decreases its levels of gene expression during ripening (Pilati et al, 2007), in tandem with the decline of IAA levels.…”

Section: Auxinsmentioning

confidence: 99%

Oxidative stress homeostasis in grapevine (Vitis vinifera L.)

Carvalho

Vidigal

Amâncio

2015

Front. Environ. Sci.

View full text Add to dashboard Cite

Plants can maintain growth and reproductive success by sensing changes in the environment and reacting through mechanisms at molecular, cellular, physiological, and developmental levels. Each stress condition prompts a unique response although some overlap between the reactions to abiotic stress (drought, heat, cold, salt or high light) and to biotic stress (pathogens) does occur. A common feature in the response to all stresses is the onset of oxidative stress, through the production of reactive oxygen species (ROS). As hydrogen peroxide and superoxide are involved in stress signaling, a tight control in ROS homeostasis requires a delicate balance of systems involved in their generation and degradation. If the plant lacks the capacity to generate scavenging potential, this can ultimately lead to death. In grapevine, antioxidant homeostasis can be considered at whole plant levels and during the development cycle. The most striking example lies in berries and their derivatives, such as wine, with nutraceutical properties associated with their antioxidant capacity. Antioxidant homeostasis is tightly regulated in leaves, assuring a positive balance between photosynthesis and respiration, explaining the tolerance of many grapevine varieties to extreme environments. In this review we will focus on antioxidant metabolites, antioxidant enzymes, transcriptional regulation and cross-talk with hormones prompted by abiotic stress conditions. We will also discuss three situations that require specific homeostasis balance: biotic stress, the oxidative burst in berries at veraison and in vitro systems. The genetic plasticity of the antioxidant homeostasis response put in evidence by the different levels of tolerance to stress presented by grapevine varieties will be addressed. The gathered information is relevant to foster varietal adaptation to impending climate changes, to assist breeders in choosing the more adapted varieties and suitable viticulture practices.

show abstract

Genome-wide transcriptional analysis of grapevine berry ripening reveals a set of genes similarly modulated during three seasons and the occurrence of an oxidative burst at vèraison

Cited by 231 publications

References 92 publications

Integrated Network Analysis Identifies Fight-Club Nodes as a Class of Hubs Encompassing Key Putative Switch Genes That Induce Major Transcriptome Reprogramming during Grapevine Development

Integrated Network Analysis Identifies Fight-Club Nodes as a Class of Hubs Encompassing Key Putative Switch Genes That Induce Major Transcriptome Reprogramming during Grapevine Development

Vascular Function in Grape Berries across Development and Its Relevance to Apparent Hydraulic Isolation

Oxidative stress homeostasis in grapevine (Vitis vinifera L.)

Contact Info

Product

Resources

About