Genome-wide transcriptome analysis between Small-tail Han sheep and the Surabaya fur sheep using high-throughput RNA sequencing

Abstract

The Small-tail Han sheep and the Surabaya fur sheep are two local breeds in North China, which are characterized by high-fecundity and low-prolificacy breed respectively. Significant genetic differences between these two breeds have provided increasing interests in the identification and utilization of major prolificacy genes in these sheep. High prolificacy is a complex trait, and it is difficult to comprehensively identify the candidate genes related to this trait using the single molecular biology technique. To understand the molecular mechanisms of fecundity and provide more information about high prolificacy candidate genes in high-and low-fecundity sheep, we explored the utility of next-generation sequencing technology in this work. A total of 1.8 Gb sequencing reads were obtained and resulted in more than 20 000 contigs that averaged w300 bp in length. Ten differentially expressed genes were further verified by quantitative real-time RT-PCR to confirm the reliability of RNA-seq results. Our work will provide a basis for the future research of the sheep reproduction.

WITHDRAWN: An integrated analysis of microRNAs involved in fat deposition in different pig breeds

“…Recently, there has been an increase in the utilization of Next Generation Sequencing (NGS) to examine the transcriptome for the identification of differential expression as well as for the opportunity to discover novel transcripts, including new alternative isoforms and miRNAs [14][15][16][17][18][19][20][21][22][23][24][25][26]. Therefore, in the present study, a comparison of the miRNA profiles of the adipose tissue of the Laiwu pig and Large White pig was performed using RNA-seq.…”

Endocrinology: Advances through omics and related technologies

“…They will also provide new insights into genes involved in the regulation of reproduction. For example, Miao and collaborators (Miao and Luo, 2013) characterized the DEGs in Small-tail Han sheep and the Surabaya fur sheep in a genomewide level using RNA-sequencing technology. They found multiple genes that were potentially critical for regulating sheep fecundity and prolificacy (Miao and Luo, 2013).…”

“…For example, Miao and collaborators (Miao and Luo, 2013) characterized the DEGs in Small-tail Han sheep and the Surabaya fur sheep in a genomewide level using RNA-sequencing technology. They found multiple genes that were potentially critical for regulating sheep fecundity and prolificacy (Miao and Luo, 2013). In fish, Devlin et al (2009) used microarrays to identify the growth traits of domesticated and GH-transgenic coho salmon.…”

Correlation between gene expression profiles in muscle and live weight in Dzhalginsky Merino sheep

“…Therefore, new candidate genes whose products function in the development of muscle fibers in sheep should be identified (Moradi et al, 2012;Miao and Luo, 2013).…”