Genomewide Gene Expression Analysis Using cDNA Microarrays

Kong, Chuang Fong; Bowtell, David D.L.

doi:10.1385/1-59259-135-3:195

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“…Chemically modified DNA probes and their conjugates play increasingly sophisticated roles in the disparate areas of biotechnology ( − ) medicine ( − ), and nanotechnology ( − ). Recent chemical literature reports the synthesis of a variety of reagents and modified solid supports that allow modifications to be introduced into the structure of chemically synthesized oligonucleotides ( − ).…”

Section: Introductionmentioning

confidence: 99%

A Modular Approach to the Synthesis of New Reagents Useful in the Chemical Synthesis of Modified DNA Probes: Derivatives of 3-(tert-Butyldimethylsiloxy)glutaric Anhydride as Versatile Building Blocks in the Synthesis of New Phosphoramidites and Modified Solid Supports

Skrzypczynski

Wayland

2004

Bioconjugate Chem.

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We present a flexible and cost-efficient synthetic strategy for the preparation of a new family of phosphoramidite and solid-support reagents that can introduce a broad range of modifications into DNA probes. The key intermediate material 3 is synthesized using the inexpensive and commercially available 3-(tert-butyldimethylsiloxy)glutaric anhydride 1 and can be used as common starting material for the preparation of new labeling reagents.

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