Genomic amplification of HPV, h‑TERC and c‑MYC in liquid‑based cytological specimens for screening of cervical intraepithelial neoplasia and cancer

Abstract

Cervical cancer is one of the most prevalent female cancer types in developing countries. ThinPrep cytological test (TCT) and human papillomavirus (HPV) detection are canonical screening methods for cervical cancer currently. However, there are limitations to these techniques. The aim of the present study was to identify efficient and practical methods for the screening of cervical intraepithelial neoplasia (CIN) and carcinoma. Residual PreservCyt specimens were obtained from 1,000 women who were admitted between August 2013 and December 2015. TCT, human telomerase RNA component (h-TERC) fluorescent in situ hybridization (FISH), MYC-specific FISH and surface plasmon resonance (SPR)-HPV genotyping were performed, followed by histopathology for those patients with positive results in any of the four tests. As a result, 106, 64, 56 and 112 patients were positive in the TCT, h-TERC, c-MYC and SPR-HPV tests, respectively, resulting in 213 being scheduled for histopathology; inflammation was identified in 159 patients, CIN I in 31, CIN II in 14, CIN III in seven and invasive cervical cancer in two patients. Using histopathology as the gold standard, TCT exhibited the highest sensitivity (87.04%), while h-TERC analysis had the highest specificity (81.76%). Parallel tests demonstrated that the Youden's index of TCT + h-TERC was the highest (0.49), while the serial analysis reported that TCT + HPV had the highest Youden's index (0.53) compared with any of the biomarkers alone (TCT, 0.50; HPV, 0.29; h-TERC, 0.47). In conclusion, dual positive TCT and HPV may be an efficient approach for basic screening of cervical lesions. h-TERC amplification may serve as an auxiliary test to improve the specificity.

Human telomerase RNA component (hTERC) gene expression and chromosome 7 ploidy correlate positively with histological grade of cervical intraepithelial neoplasia

“…The proportion of cases with hTERC gene overexpression showed a significant increment with progressively increasing cytological and histological abnormalities, findings akin to previous studies 10,20,21,25,33,34,36‐40 . In fact, most earlier publications on hTERC expression have focussed on the frequency of hTERC positivity in various cytology and/or histology categories, deriving their cut‐offs from percentage of hTERC‐expressing cells in normal/ negative samples.…”

Prediction of the Mechanisms by Which Quercetin Enhances Cisplatin Action in Cervical Cancer: A Network Pharmacology Study and Experimental Validation

“…CCND1 belongs to the highly conserved cyclin family and plays a key role in the cell cycle transition from G1 phase to S phase transition ( 59 ). MYC is a proto-oncogene that encodes a nuclear phosphoprotein and is involved in cell proliferation, the cell cycle, and apoptosis ( 60 ). However, there have been no reports regarding the role of the four genes in quercetin treatment of cervical cancer.…”

Polydatin Inhibits Cell Viability, Migration, and Invasion Through Suppressing the c-Myc Expression in Human Cervical Cancer

“…In the process of normal cell proliferation, the expression of c-Myc is strictly controlled, but in malignancies of different histogenetic origins, the increased c-Myc expression is often observed. In cervical cancer, recent reports have indicated that the c-Myc expression increases with increasing severity of histological diagnosis (Heng et al, 2018;Ji et al, 2018); these results emphasize the potential function of c-Myc as an indicator in cervical cancer (Li et al, 2014;Gao et al, 2015;. In this study, the expression of the proto-oncogene c-Myc was notably decreased in a dose-and time-dependent manner at both the mRNA and protein levels in two human cervical cancer cell lines (CaSki and C33A) after PD treatment.…”