Genomic analyses of metal resistance genes in three plant growth promoting bacteria of legume plants in Northwest mine tailings, China

Xie, Pin; Hao, Xiuli; Herzberg, Martin; Luo, Yane; Nies, Dietrich H.

doi:10.1016/j.jes.2014.07.017

Cited by 39 publications

(17 citation statements)

References 46 publications

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“…Chryseobacterium solincola can grow despite the elevated presence of highly toxic metals and has been suggested as a candidate for the situ bioremediation of heavy metals in aqueous or soil systems (50). Agrobacterium tumefaciens is able to survive in regions containing high levels of heavy metals and possesses various transporters involved in Ni, Cu, Zn and Cr resistance (51, 52). SVs of the genera Cryocola and Perlucidibaca significantly decreased in abundance in both Cd treatments.…”

Section: Discussionmentioning

confidence: 99%

Earthworms and Cadmium - heavy metal resistant gut bacteria as indicators for heavy metal pollution in soils?

Šrut

Menke

Höckner

et al. 2018

Preprint

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Preservation of the soil resources stability is of paramount importance for the ecosystem, particularly in the current era of environmental change, which presents a severe pollution burden (e.g. by heavy metals) to soil ecosystems and its fauna. Gut microbiomes are becoming recognized as important players in organism health, with comprehension of their perturbations in the polluted environment offering new insights into the nature and extent of heavy metal effects on the health of soil biota. Our aim was therefore to evaluate the effect of environmentally relevant heavy metal concentrations of cadmium (Cd) on the earthworm gut microbiota. Cd exposure led to perturbations of several heavy metal resistant taxa as well as taxa able to bind heavy metals, revealing the potential of the earthworm-microbiota system in overcoming humancaused heavy metal pollution. An ‘indicator species analysis’ linked bacterial genera Paenibacillus and Flavobacterium, and members of the order Actinomycetales with Cd treatment, suggesting the possible use of these bacterial taxa as biomarkers of exposure for Cd stressed soils. The results of this study will be essential to understanding of the soil fauna health, under anthropogenic disturbance, and will have implications for environmental monitoring and protection of soil resources.ImportanceSoil heavy metal pollution presents a severe burden for soil invertebrates and can have impact on their health, which in turn reflects on the health of the entire ecosystem. Gut microbiome is recognized as a central driver of the host health and its shifts can have severe consequences for the host. In this study we investigated the impact of cadmium (Cd) on earthworm gut microbiota, in a controlled experiment using cutting edge next generation sequencing and state of the art bioinformatics tools. The significance of this study is in identifying the gut bacterial taxa which are indicators for Cd treatment and are potential biomarkers of exposure to Cd. Therefore, this study contributes to develop efficient measures to qualify environmental pollution and to protect fragile soil resources and ultimately human health.

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Section: Discussionmentioning

confidence: 99%

Earthworms and Cadmium - heavy metal resistant gut bacteria as indicators for heavy metal pollution in soils?

Šrut

Menke

Höckner

et al. 2018

Preprint

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“…Therefore, the utilization of microorganisms in contaminated environments represents a promising solution for metal remediation [58,59,60,61,62,63,64]. Findings of the present work imply the potential usage of S. meliloti in improvement growth of alfalfa plants in metal/metalloid contaminated soil, providing evidence of the key role of EPSs as a probable molecular determinant of bacterial survival in metal stressed soils and consequently allowing the development of symbiosis in such polluted environments.…”

Section: Discussionmentioning

confidence: 99%

Roles of Extracellular Polysaccharides and Biofilm Formation in Heavy Metal Resistance of Rhizobia

et al. 2016

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Bacterial surface components and extracellular compounds, particularly flagella, lipopolysaccharides (LPSs), and exopolysaccharides (EPSs), in combination with environmental signals and quorum-sensing signals, play crucial roles in bacterial autoaggregation, biofilm development, survival, and host colonization. The nitrogen-fixing species Sinorhizobium meliloti (S. meliloti) produces two symbiosis-promoting EPSs: succinoglycan (or EPS I) and galactoglucan (or EPS II). Studies of the S. meliloti/alfalfa symbiosis model system have revealed numerous biological functions of EPSs, including host specificity, participation in early stages of host plant infection, signaling molecule during plant development, and (most importantly) protection from environmental stresses. We evaluated functions of EPSs in bacterial resistance to heavy metals and metalloids, which are known to affect various biological processes. Heavy metal resistance, biofilm production, and co-culture were tested in the context of previous studies by our group. A range of mercury (Hg II) and arsenic (As III) concentrations were applied to S. meliloti wild type strain and to mutant strains defective in EPS I and EPS II. The EPS production mutants were generally most sensitive to the metals. Our findings suggest that EPSs are necessary for the protection of bacteria from either Hg (II) or As (III) stress. Previous studies have described a pump in S. meliloti that causes efflux of arsenic from cells to surrounding culture medium, thereby protecting them from this type of chemical stress. The presence of heavy metals or metalloids in culture medium had no apparent effect on formation of biofilm, in contrast to previous reports that biofilm formation helps protect various microorganism species from adverse environmental conditions. In co-culture experiments, EPS-producing heavy metal resistant strains exerted a protective effect on AEPS-non-producing, heavy metal-sensitive strains; a phenomenon termed “rescuing” of the non-resistant strain.

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“…Genomic analysis of three legume growth-promoting rhizobia ( Mesorhizobium amorphae CCNWGS0123, Sinorhizobium meliloti CCNWSX0020 and Agrobacterium tumefaciens CCNWGS0286) that survive at regions with high levels of heavy metals in China confirmed the existence of different transporters involved in nickel, copper, zinc, and chromate resistance ( Xie P. et al, 2015 ). In another report, Zhao et al (2015) used an RNA-seq approach to investigate the genes associated with Cd stress in the Dark Septate Endophytic (DSE) fungal Cd-tolerant strain Exophiala pisciphila isolated from the roots of the Poaceae plant Arundinella bengalensis.…”

Section: Rhizoremediation: the Combinatorial Effects Of Bio/phytoremementioning

confidence: 96%

“…Similarly, RNA-Seq and de novo transcriptome analysis showed that 1561 unigenes were down-regulated and 1424 unigenes were up-regulated, respectively, in radish roots under chromium stress ( Xie Y. et al, 2015 ). Here, several transcription factors such as Cr stress-responsive genes involved in signal transduction, chelate compounds and antioxidant biosynthesis have been proposed ( Xie P. et al, 2015 ). Furthermore, the degradome sequencing approach has been used to identify miRNAs and their target genes under Pb stress in Platanus acerifolia tree plants ( Wang et al, 2015 ).…”

Section: Potential Strategies For Phytoremediationmentioning

confidence: 99%

Potential Biotechnological Strategies for the Cleanup of Heavy Metals and Metalloids

et al. 2016

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Global mechanization, urbanization, and various natural processes have led to the increased release of toxic compounds into the biosphere. These hazardous toxic pollutants include a variety of organic and inorganic compounds, which pose a serious threat to the ecosystem. The contamination of soil and water are the major environmental concerns in the present scenario. This leads to a greater need for remediation of contaminated soils and water with suitable approaches and mechanisms. The conventional remediation of contaminated sites commonly involves the physical removal of contaminants, and their disposition. Physical remediation strategies are expensive, non-specific and often make the soil unsuitable for agriculture and other uses by disturbing the microenvironment. Owing to these concerns, there has been increased interest in eco-friendly and sustainable approaches such as bioremediation, phytoremediation and rhizoremediation for the cleanup of contaminated sites. This review lays particular emphasis on biotechnological approaches and strategies for heavy metal and metalloid containment removal from the environment, highlighting the advances and implications of bioremediation and phytoremediation as well as their utilization in cleaning-up toxic pollutants from contaminated environments.

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Genomic analyses of metal resistance genes in three plant growth promoting bacteria of legume plants in Northwest mine tailings, China

Cited by 39 publications

References 46 publications

Earthworms and Cadmium - heavy metal resistant gut bacteria as indicators for heavy metal pollution in soils?

Earthworms and Cadmium - heavy metal resistant gut bacteria as indicators for heavy metal pollution in soils?

Roles of Extracellular Polysaccharides and Biofilm Formation in Heavy Metal Resistance of Rhizobia

Potential Biotechnological Strategies for the Cleanup of Heavy Metals and Metalloids

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