Genomic analysis and assessment of pathogenic (toxicogenic) potential of Staphylococcus haemolyticus and Bacillus paranthracis consortia isolated from bovine mastitis in Russia

Abstract

Three stable microbial consortia, each composed of Bacillusparanthracis and Staphylococcushaemolyticus strains, were isolated from milk of cows diagnosed with mastitis in three geographically remote regions of Russia. The composition of these consortia remained stable following multiple passages on culture media. Apparently, this stability is due to the structure of the microbial biofilms formed by the communities. The virulence of the consortia depended on the B. paranthracis strains. It seems plausible that the ability of the consortia to cause mastitis in cattle was affected by mutations of the cytK gene of B. paranthracis.

Exploring the Genomic Landscape of Bacillus paranthracis PUMB_17 as a Proficient Phosphatidylcholine-Specific Phospholipase C Producer

“…These associated proteins usually serve to enhance the cytolytic protein’s biological effectiveness, as is also observed in other species strains. In contrast, PUMB_17 lacked the virulence genes hblA and cytK2 , observed in recently identified pathogen strains B. paranthracis 4M, 1702, and 1710 [ 55 ]. In the protease category, immune inhibitor A metalloprotease ( inhA , QRA03_15915) was detected; this gene assists in surviving the host environment conditions.…”

“…Originally isolated from sediment in the Pacific Ocean [ 10 ], Bacillus paranthracis has been identified in human feces, with documented associations to instances of diarrhea [ 60 , 61 ]. Additionally, the bacterium has been implicated in cases of bovine mastitis, underscoring its potential pathogenicity [ 55 ]. However, the characterization of B. paranthracis remains incomplete, with a paucity of publications employing whole-genome sequencing to explore its strains comprehensively.…”

Whole Genome Sequencing Reveals Antimicrobial Resistance and Virulence Genes of Both Pathogenic and Non-Pathogenic B. cereus Group Isolates from Foodstuffs in Thailand

“…In 2020, B. paranthracis was isolated from blood of a fatal Ebola virus disease case in Liberia and was identified by whole genome sequencing [ 35 ]. B. paranthracis is considered as a pathogenic bacterium both in human and animal hosts since it could be isolated from the milk of cows diagnosed with mastitis [ 36 ]. It is also known as emetic B. cereus due to the ability to produce cereulide, an emetic toxin.…”

Isolation and Identification of Antibiotic-Susceptible Bacteria from Abattoir Effluent in Port Harcourt, Rivers State, Nigeria

“…The bacterial isolates harbouring the resistance gene QnrA are A. brisouii and B. altitudinis while P. flexa, A. brisouii and B. stratosphericus harboured icaC gene for adhesion. Sokolov [26] detected icaC in Staphylococcus species but not in Bacillus. The icaC gene is part of icaABCD cluster under the control ica operon, known for the synthesis of polysaccharide intercellular adhesin (PIA), a major component of the extracellular matrix necessary for biofilm formation by bacteria [27,28].…”