Genomic analysis of a novel strain of Bacillus nealsonii, isolated from Surti buffalo rumen

Nathani, Neelam M.; Duggirala, Srinivas M.; Bhatt, Vaibhav D.; KaPatel, Jay; Joshi, Chaitanya

doi:10.4236/abb.2014.53030

Cited by 8 publications

(4 citation statements)

References 31 publications

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“…Although many Bacillus strains were tested previously, it is clear that numerous species remain undiscovered and not officially described [ 55 , 56 ]. B. nealsonii was already isolated from potentially hostile environments, including a spacecraft assembly and the rumen of a buffalo [ 57 , 58 ], and it is not well characterized or shown great usefulness except for its very limited application to the production of proteases and as an additive detergent [ 59 , 60 ]. The secondary metabolites of the strain Sen132 of B. nealsonii that we isolated were screened for mosquitocidal activity and exhibited high activity against A. albopictus larvae.…”

Section: Discussionmentioning

confidence: 99%

Biological Control of Aedes albopictus: Obtained from the New Bacterial Candidates with Insecticidal Activity

Dahmana

Sambou

Raoult

et al. 2020

Insects

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Vector-borne deadly pathogens cause more than 700,000 deaths annually. They are transmitted by several vectors, among which the mosquito is the most important. Chemical compounds often have devastating side effects, leading to the abandonment of the majority of them. Biological control has been performed by using formulations of Bacillus sphaericus and Bacillus thuringiensis, but their intensive use has led to the emergence of resistance. Currently, the development of new alternative molecules is urgently needed, in order to use them in mosaics or in rotation with already known insecticides for the control of vectors, especially mosquitoes. Here, we attempted to identify bacterial species with potential anti-mosquito actions. Among bacterial strains isolated from dry sandy soil from Senegal, eleven strains from the Bacillales and Actinomycetales orders were chosen for the entomopathogenic activity experiments. Then, we tested their secondary metabolites, which were obtained from the supernatant fraction, and their cell wall and cytoplasmic compounds, which were found in the pellet fraction, in Aedes albopictus larvae, and compared the larval mortality rate with that obtained by using a commercial product. A total of 4/11 (36.36%) of the isolated species exhibited insecticidal activity. B. nealsonii, which is not a well-known bacterium, had the highest larvicidal effect with 70% of the larval mortality, which is highlighted for the first time. The Streptomyces species we isolated seem to be potential new species, and 3/5 (60%) of them exhibited insecticidal activity. Our study reports provide potential candidates for the identification of active molecules to be developed for strengthening the biological control of infectious diseases agents transmitted by mosquitoes.

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Section: Discussionmentioning

confidence: 99%

Biological Control of Aedes albopictus: Obtained from the New Bacterial Candidates with Insecticidal Activity

Dahmana

Sambou

Raoult

et al. 2020

Insects

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“…Although many Bacillus strains have already been tested, it is clear that numerous species have not yet been discovered and officially described 48,49 . B. nealsonii has already been isolated from potentially hostile environments, including a spacecraft assembly and the rumen of a buffalo 50,51 , and it has not been well characterised or shown great usefulness except for its very limited application to the production of proteases and as an additive detergent 52,53 . The secondary metabolites of the strain Sen132 of B.…”

Section: Discussionmentioning

confidence: 99%

Insecticidal activity of the metabolites of sand soil bacteria in Northern Senegal

Dahmana¹,

Sambou²,

Raoult³

et al. 2020

Preprint

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BackgroundVector-borne deadly diseases cause more than 700,000 deaths annually. They are transmitted by several vectors, among which the mosquito is the most important. Chemical compounds often have devastating side effects, which has led to the abandonment of the majority of them. Biological control has been performed by using formulations of Bacillus sphaericus and Bacillus thuringiensis, but their intensive use has led to the emergence of resistance. Currently, there is an urgent need to develop new alterative molecules from bacteria to use in mosaics or in rotation with already known insecticides and bacteria for the control of vectors, especially mosquitos. Here, we attempted to identify bacterial species with potential anti-mosquito actions.ResultsAmong 208 strains isolated from dry sandy soil from Senegal, eleven bacterial strains from the Bacillus genus (5) and Actinomycetales order (6) were chosen for the following experiments. Then, we tested their secondary metabolites, which were obtained from the supernatant fraction, and their cell wall and cytoplasmic compounds, which were found in the pellet fraction, in 3rd and early 4th Aedes albopictus larvae instars and compared the larval mortality rate with that obtained by using a commercial product. A total of 4/11 (36.4%) of the isolated species exhibited insecticidal activity. B. nealsonii, which is not a well-known bacterium, had the highest larvicidal effect with 70% larval mortality, which highlighted for the first time. The Streptomyces spp. we isolated seem to be potential new species, and 3/5 (60%) of them exhibited insecticidal activity.ConclusionThe bacterial strains isolated in this study may be used to identify the molecular mechanisms of their insecticidal activity.

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“…The extracted DNA was stored at a temperature of -20 °C. To amplify the 16S rDNA region, universal primers fD1 (5′-AAG GAG GTG ATC CAG CC-3′) and rD1 (5′-AGA GTT TGA TCC TGG CT C AG-3′) were used (Nathani et al., 2014). Thermal reaction of PCR with the thermal program was performed (Assareh et al., 2012).…”

Section: Methodsmentioning

confidence: 99%

Biological removal of iron and sulfate from synthetic wastewater of cotton delinting factory by using halophilic sulfate-reducing bacteria

Torbaghan

Torghabeh

2019

Heliyon

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Industrial and agricultural wastewater treatment, which has the potential to cause serious risks to human health and the environment, has special importance at the lowest cost and highest efficiency such as biological processes to treat wastewater. The purpose of the study was removing iron and sulfate from very saline synthetic wastewater by means of halophilic sulfate-reducing bacteria. This process was performed under anaerobic conditions to change wastewater to a chemical fertilizer to use in saline and alkaline soils. Three halophilic SRBs were isolated and purified from wastewater of the cotton delinting factory by Postage C medium which supplemented with sodium chloride and magnesium chloride hexahydrate. The highest NaCl tolerance strain (HSR973) was allocated to Desulfovibrio halophilus sp. This experimental study was conducted in a fluid bed reactor at anaerobic conditions. Diluted concentrations of cotton linters wastewater containing 50–400 ppm iron were added to the reactor. After the bacteria fixation to different iron concentrations, the maximum removal efficiency of iron and sulfate was achieved 85.3 % and 78.4 % at the optimum retention time of 24-hours respectively. Sulfate concentration in samples decreased to about 20 % of initial concentration after 24-h retention time. The highest production of H2S at optimum operational conditions was about 228 ml l−1. The reduction of sulfate and iron biological precipitation by anaerobic rector presented high performance. This removing accompanied with the alkalinity increase during the process which could be improved condition for acidic wastewater treatment. The produced iron sulfide sludge was not suitable for use as a chemical fertilizer due to its lack of complete separation. However, the total sludge produced was able to be consumed in saline and alkaline soils for various purposes after additional treatment.

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Genomic analysis of a novel strain of Bacillus nealsonii, isolated from Surti buffalo rumen

Abstract: ABSTRACT

Cited by 8 publications

References 31 publications

Biological Control of Aedes albopictus: Obtained from the New Bacterial Candidates with Insecticidal Activity

Biological Control of Aedes albopictus: Obtained from the New Bacterial Candidates with Insecticidal Activity

Insecticidal activity of the metabolites of sand soil bacteria in Northern Senegal

Biological removal of iron and sulfate from synthetic wastewater of cotton delinting factory by using halophilic sulfate-reducing bacteria

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