Genomic Analysis of Chilean Strains of<i>Campylobacter jejuni</i>from Human Faeces

Levicán, Arturo; Ramos-Tapia, Ignacio; Briceño, Isabel; Guerra, Francisco; Mena, Benjamin; Varela, Carmen; Porte, Lorena

doi:10.1155/2019/1902732

Cited by 20 publications

(30 citation statements)

References 48 publications

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“…In addition to CC-21, we identified high prevalence rates of CC-48 (13%) and CC-52 (7.3%). ST-475 from CC-48 was the second most prevalent ST (11.6%) identified (Figure 1A).There are only two previous reports of MLST data of clinical C. jejuni strains from Chile[18,22]. Both reports identified four major CCs and STs, including CC-21 (ST-50), CC-48 (ST-475), CC-257 (ST-257), and CC-353 (ST-353).…”

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confidence: 89%

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Genomic analysis of the diversity, antimicrobial resistance and virulence potential of clinical Campylobacter jejuni and Campylobacter coli strains from Chile

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Porte

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ABSTRACTCampylobacter jejuni and Campylobacter coli are the leading causes of human gastroenteritis in the developed world and an emerging threat in developing countries. There is increasing evidence that the incidence of campylobacteriosis in Chile and other South American countries is greatly underestimated, mostly due to the lack of adequate diagnostic methods. Accordingly, there is limited genomic and epidemiological data from these countries, leaving an important knowledge gap in our understanding of the population structure, virulence potential, and antimicrobial resistance profiles of clinical Campylobacter strains. We recently published the whole-genome sequences of a collection of 68 C. jejuni and 12 C. coli strains isolated from patients with enteric infections during a 2-year period (2017-2019) attending Clinica Alemana in Santiago, Chile. In the present study, we sequenced an additional C. jejuni genome and performed a genome-wide analysis of the genetic diversity, virulence, and antimicrobial resistance potential of these 81 strains. cgMLST analysis identified a high genetic diversity of C. jejuni as well as 13 novel STs and alleles in both C. jejuni and C. coli. Pangenome and virulome analyses showed a differential distribution of virulence factors, including both plasmid and chromosomally encoded T6SSs and T4SSs. Resistome analysis predicted widespread resistance to fluoroquinolones, but a low rate of erythromycin resistance. This study provides valuable genomic and epidemiological data and highlights the need for larger genomic epidemiology studies to better understand the genetic diversity, virulence potential and antimicrobial resistance of human clinical Campylobacter strains in South America.DATA SUMMARYGenome sequences and assemblies of all C. jejuni and C. coli used in this study have been deposited in public databases (Table S1) and most strains have also been published as genome resource announcements [1,2].Bacterial draft genome sequence reannotations and nucleotide sequence fasta files used for virulome and plasmid screening analysis are available as a supplementary dataset on Zenodo (DOI: 10.5281/zenodo.3925206) https://doi.org/10.5281/zenodo.3925206.The reconstructed phylogenetic trees and associated metadata of clinical strains from Chile in the global context of C. jejuni and C. coli sequenced strains can be interactively visualized on Microreact at https://microreact.org/project/VbEQsZtQD.

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confidence: 89%

“…There is only one report that used whole-genome sequence data (wgMLST) to determine the genetic diversity of C. jejuni strains from the country, which was based on the first 3 genomes of Chilean C. jejuni strains. Therefore larger genomic epidemiology studies are needed [22].…”

Section: Introductionmentioning

confidence: 99%

Genomic analysis of the diversity, antimicrobial resistance and virulence potential of clinical Campylobacter jejuni and Campylobacter coli strains from Chile

Bravo

Katz

Porte

et al. 2020

Preprint

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“…Previous survey studies have suggested that the cadF and flpA genes are conserved amongst C. jejuni isolates (Konkel et al, 1999a;Ripabelli et al, 2010;Ghorbanalizadgan et al, 2014;Levican et al, 2019;Wei et al, 2019). To explore this proposition, we assessed 20,218 full C. jejuni genome sequences that were available on the GenBank FTP server as of August 1st, 2019.…”

Section: Presence Of Cadf and Flpa In C Jejuni Isolatesmentioning

confidence: 99%

“…coli-specific diagnostic marker. In contrast to other potential virulence-associated genes [e.g., the CDT subunit genes (cdtA, cdtB, and cdtC), iamA, iamB, and virB11], cadF is present in nearly 100% of C. jejuni and C. coli isolates (Supplementary Figure S1) (Ghorbanalizadgan et al, 2014;Levican et al, 2019;Wei et al, 2019). The cadF and flpA genes do not appear to be present in other pathogenic bacteria.…”

Section: Presence Of Cadf and Flpa In C Jejuni Isolatesmentioning

confidence: 99%

“…The cadF and flpA genes do not appear to be present in other pathogenic bacteria. Given that molecular diagnostics is becoming a more common approach, we have cited only a few articles where researchers have used PCR amplification of the cadF gene for diagnostic purposes (Ghorbanalizadgan et al, 2014;Francois et al, 2018;Levican et al, 2019;Wei et al, 2019).…”

Section: Presence Of Cadf and Flpa In C Jejuni Isolatesmentioning

confidence: 99%

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Taking Control: Campylobacter jejuni Binding to Fibronectin Sets the Stage for Cellular Adherence and Invasion

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Campylobacter jejuni, a foodborne pathogen, is one of the most common bacterial causes of gastroenteritis in the world. Undercooked poultry, raw (unpasteurized) dairy products, untreated water, and contaminated produce are the most common sources associated with infection. C. jejuni establishes a niche in the gut by adhering to and invading epithelial cells, which results in diarrhea with blood and mucus in the stool. The process of colonization is mediated, in part, by surface-exposed molecules (adhesins) that bind directly to host cell ligands or the extracellular matrix (ECM) surrounding cells. In this review, we introduce the known and putative adhesins of the foodborne pathogen C. jejuni. We then focus our discussion on two C. jejuni Microbial Surface Components Recognizing Adhesive Matrix Molecule(s) (MSCRAMMs), termed CadF and FlpA, which have been demonstrated to contribute to C. jejuni colonization and pathogenesis. In vitro studies have determined that these two surface-exposed proteins bind to the ECM glycoprotein fibronectin (FN). In vivo studies have shown that cadF and flpA mutants exhibit impaired colonization of chickens compared to the wildtype strain. Additional studies have revealed that CadF and FlpA stimulate epithelial cell signaling pathways necessary for cell invasion. Interestingly, CadF and FlpA have distinct FN-binding domains, suggesting that the functions of these proteins are nonredundant. In summary, the binding of FN by C. jejuni CadF and FlpA adhesins has been demonstrated to contribute to adherence, invasion, and cell signaling.

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