Genomic Analysis of Klebsiella pneumoniae ST258 Strain Coproducing KPC-2 and CTX-M-14 Isolated from Poultry in the Brazilian Amazon Region

Valiatti, Tiago Barcelos; Cayô, Rodrigo; Santos, Fernanda dos; Bessa-Neto, Francisco Ozório; Silva, Ramon Giovani Brandão; Veiga, Ruanita; Bahia, Márcia de Nazaré Miranda; Guerra, Lívia Maria Guimarães Dutra; Pignatari, Antônio Carlos Campos; Souza, Cintya de Oliveira; Brasiliense, Danielle Murici; Gales, Ana Cristina

doi:10.3390/antibiotics11121835

Cited by 4 publications

(1 citation statement)

References 33 publications

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“…KPC-producing isolates represent an important public health issue. Recently, emerging data on food-producing animals and their products, as reservoirs for KPC-producing isolates, are also being reported in different countries [ 22 , 23 ]. New beta-lactams/beta-lactamase inhibitors are now considered a first-line treatment option against KPC-producing isolates.…”

Section: Discussionmentioning

confidence: 99%

Synergistic Activity of Temocillin and Fosfomycin Combination against KPC-Producing Klebsiella pneumoniae Clinical Isolates

Costantino,

Principe,

Mehat

et al. 2024

Antibiotics

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Infections caused by KPC-producing K. pneumoniae continue to pose a significant clinical challenge due to their emerging resistance to new antimicrobials. We investigated the association between two drugs whose roles have been repurposed against multidrug-resistant bacteria: fosfomycin and temocillin. Temocillin exhibits unusual stability against KPC enzymes, while fosfomycin acts as a potent “synergizer”. We conducted in vitro antimicrobial activity studies on 100 clinical isolates of KPC-producing K. pneumoniae using a combination of fosfomycin and temocillin. The results demonstrated synergistic activity in 91% of the isolates. Subsequently, we assessed the effect on Galleria mellonella larvae using five genetically different KPC-Kp isolates. The addition of fosfomycin to temocillin increased larvae survival from 73 to 97% (+Δ 32%; isolate 1), from 93 to 100% (+Δ 7%; isolate 2), from 63 to 86% (+Δ 36%; isolate 3), from 63 to 90% (+Δ 42%; isolate 4), and from 93 to 97% (+Δ 4%; isolate 10). Among the temocillin-resistant KPC-producing K. pneumoniae isolates (24 isolates), the addition of fosfomycin reduced temocillin MIC values below the resistance breakpoint in all isolates except one. Temocillin combined with fosfomycin emerges as a promising combination against KPC-producing K. pneumoniae, warranting further clinical evaluation.

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Section: Discussionmentioning

confidence: 99%

Synergistic Activity of Temocillin and Fosfomycin Combination against KPC-Producing Klebsiella pneumoniae Clinical Isolates

Costantino,

Principe,

Mehat

et al. 2024

Antibiotics

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show abstract

Unraveling genetic similarities of Klebsiella pneumoniae from poultry and poultry handlers in Punjab, India

Parmar,

Singh,

Kaur

et al. 2025

Gene Reports

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Synergistic Activity of Temocillin and Fosfomycin Combination against KPC-Producing <em>Klebsiella pneumoniae </em>Clinical Isolates

Costantino,

Principe,

Mehat

et al. 2024

Preprint

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Infections caused by KPC-producing K. pneumoniae continue to pose a significant clinical challenge due to emerging resistance to new antimicrobials. We investigated the association between two drugs whose roles have been repurposed against multidrug-resistant bacteria: fosfomycin and temocillin. Temocillin exhibits unusual stability against KPC enzymes, while fosfomycin acts as a potent “synergizer”. We conducted in vitro antimicrobial activity studies on 100 clinical isolates of KPC-producing K. pneumoniae using a combination of fosfomycin and temocillin. The results demonstrated synergistic activity in 91% of the isolates. Subsequently, we assessed the effect on Galleria mellonella larvae using 5 different KPC-Kp isolates. The addition of fosfomycin to temocillin increased larvae survival from 73 to 97% (+Δ 32%; isolate 1), from 93 to 100% (+Δ 7%; isolate 2), from 63 to 86% (+Δ 36%; isolate 3), from 63 to 90% (+Δ 42%; isolate 4), and from 93 to 97% (+Δ 4%; isolate 10), respectively. Among the temocillin-resistant KPC-producing K. pneumoniae isolates (24 isolates), the addition of fosfomycin reduced temocillin MIC values below the resistance breakpoint in all isolated except one. Temocillin combined with fosfomycin emerges as a promising combination against KPC-producing K. pneumoniae, warranting further clinical evaluation.

show abstract

Genomic Analysis of Klebsiella pneumoniae ST258 Strain Coproducing KPC-2 and CTX-M-14 Isolated from Poultry in the Brazilian Amazon Region

Cited by 4 publications

References 33 publications

Synergistic Activity of Temocillin and Fosfomycin Combination against KPC-Producing Klebsiella pneumoniae Clinical Isolates

Synergistic Activity of Temocillin and Fosfomycin Combination against KPC-Producing Klebsiella pneumoniae Clinical Isolates

Unraveling genetic similarities of Klebsiella pneumoniae from poultry and poultry handlers in Punjab, India

Synergistic Activity of Temocillin and Fosfomycin Combination against KPC-Producing <em>Klebsiella pneumoniae </em>Clinical Isolates

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