Genomic Analysis of Limosilactobacillus fermentum ATCC 23271, a Potential Probiotic Strain with Anti-Candida Activity

Abstract

Limosilactobacillus fermentum (ATCC 23271) was originally isolated from the human intestine and has displayed antimicrobial activity, primarily against Candida species. Complete genome sequencing and comparative analyses were performed to elucidate the genetic basis underlying its probiotic potential. The ATCC 23271 genome was found to contain 2,193,335 bp, with 2123 protein-coding sequences. Phylogenetic analysis revealed that the ATCC 23271 strain shares 941 gene clusters with six other probiotic strains of L. fermentum. Putative genes known to confer probiotic properties have been identified in the genome, including genes related to adhesion, tolerance to acidic pH and bile salts, tolerance to oxidative stress, and metabolism and transport of sugars and other compounds. A search for bacteriocin genes revealed a sequence 48% similar to that of enterolysin A, a protein from Enterococcus faecalis. However, in vitro assays confirmed that the strain has inhibitory activity on the growth of Candida species and also interferes with their adhesion to HeLa cells. In silico analyses demonstrated a high probability of the protein with antimicrobial activity. Our data reveal the genome features of L. fermentum ATCC 23271, which may provide insight into its future use given the functional benefits, especially against Candida infections.

Probiotic potential of Vaginal flora from healthy Indian women against Urinary pathogens

“…S5). On the contrary, all L. fermentum strains displayed > 90% survival rates at all pH tested, which was accordance with previous reports [44,45]. Intriguingly, strains that displayed enhanced survival in the planktonic state under high acidic conditions were unable to form bio lms at acidic pH, increased protonation might have contributed to masking of cell surface charge which could possibly play a role in preventing initial attachment to static surfaces.…”

Functional genomics of the lactic acid bacterium Limosilactobacillus fermentum LAB-1: metabolic, probiotic and biotechnological perspectives

“…Consistent with these results, the functional genome analysis of other L. fermentum strains, for example, ATCC 23271 and ING8 also showed similar annotation properties with their 2123 and 2103 coding sequences distributed across 312 and 208 subsystems respectively [ 38 , 39 ]. Moreover, similar to LAB-1, a large proportion of the subsystem features was dedicated to protein metabolism, amino acids and derivatives, and carbohydrates.…”

“…In fact, there appears to be only a few experimentally validated antimicrobial peptides reported in L. fermentum . Most recently, a gene originally annotated as a hypothetical protein in L. fermentum 23271 strain showed 48% sequence similarity to enterolysin A, a bacteriocin produced by Enterococcus faecalis which inhibited species of Enterococcus, Lactobacillus, Lactococcus and Pediococcus [ 38 ]. The functional genomics of the L. fermentum strain SK152 led to the identification of another putative antimicrobial gene encoding a putative endolysin [ 74 ].…”

Whole genome analysis of host-associated lactobacillus salivarius and the effects on hepatic antioxidant enzymes and gut microorganisms of Sinocyclocheilus grahami

“…Two bacteriocin gene clusters, Enterolysin_A and sakacin_G_skgA1, were detected in the BAGEL4 database. Enterolysin_A gene cluster encodes a cell wall degrading bacteriocin, a class III bacteriocin ( Dos Santos et al, 2021 ; Nilsen et al, 2003 ). The sakacin G bacteriocin encoded by the sakacin_G_skgA1 gene cluster can lyse sensitive cells, leading to the leakage of enzymes and DNA, thereby inducing apoptosis in bacteria ( Todorov et al, 2011 ).…”