Genomic Analysis of Propane Metabolism in Methyl <i>Tert</i>-Butyl Ether-Degrading <i>Mycobacterium</i> Sp. Strain ENV421

Tupa, Peter R.; Masuda, Hisako

doi:10.7150/jgen.24929

Cited by 12 publications

(4 citation statements)

References 30 publications

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“…ENV421 and Rhodococcus rhodochrous 21198 , (Table ). Further, the presence of propane can also upregulate the polycistronic transcription of the prmABCD clusters in PH-06 and ENV421, , which subsequently promoted the activity of dioxane biotransformation. Our study revealed that this single PRM enzyme can degrade both dioxane and gaseous alkanes.…”

Section: Resultsmentioning

confidence: 99%

Distinct Catalytic Behaviors between Two 1,4-Dioxane-Degrading Monooxygenases: Kinetics, Inhibition, and Substrate Range

Deng

2019

Environ. Sci. Technol.

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Monitored natural attenuation (MNA) and engineered bioremediation have been recognized as effective and cost-efficient in situ treatments to mitigate 1,4-dioxane (dioxane) contamination. Dioxane metabolism can be initiated by two catabolic enzymes, propane monooxygenase (PRM) and tetrahydrofuran monooxygenase (THM), belonging to the group-6 and 5 of soluble di-iron monooxygenase family, respectively. In this study, we comprehensively compared catalytic behaviors of PRM and THM when individually expressed in the heterologous host, Mycobacterium smegmatis mc 2 -155. Kinetic results revealed a half-saturation coefficient (K m ) of 53.0 ± 13.1 mg/L for PRM, nearly 4 times lower than that of THM (235.8 ± 61.6 mg/L), suggesting that PRM has a higher affinity to dioxane. Exposure with three common co-contaminants (1,1dichloroethene, trichloroethene, and 1,1,1-trichloroethane) demonstrated that PRM was also more resistant to their inhibition than THM. Thus, dioxane degraders expressing PRM may be more physiologically and ecologically advantageous than those with THM at impacted sites, where dioxane concentration is relatively low (e.g., 250 to 1000 μg/L) with co-occurrence of chlorinated solvents (e.g., 0.5 to 8 mg/L), underscoring the need of surveying both PRM and THM-encoding genes for MNA potential assessment. PRM is also highly versatile, which breaks down cyclic molecules (dioxane, tetrahydrofuran, and cyclohexane), as well as chlorinated and aromatic pollutants, including vinyl chloride, 1,2-dichloroethane, benzene, and toluene. This is the first report regarding the ability of PRM to degrade a variety of short-chain alkanes and ethene in addition to dioxane, unraveling its pivotal role in aerobic biostimulation that utilizes propane, isobutane, or other gaseous alkanes/alkenes (e.g., ethane, butane, and ethene) to select and fuel indigenous microorganisms to tackle the commingled contamination of dioxane and chlorinated compounds.

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Section: Resultsmentioning

confidence: 99%

Distinct Catalytic Behaviors between Two 1,4-Dioxane-Degrading Monooxygenases: Kinetics, Inhibition, and Substrate Range

Deng

2019

Environ. Sci. Technol.

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“…Another study has shown that MTBE and other ethers were used as a carbon source during the growth of propane by some microorganisms such as Mycobacterium spp. [31]. Peter Roslev and coworkers demonstrated the lack of androgenic response and weak estrogenic response in S. cerevisiae by exposing to MTBE [2].…”

Section: Comentioning

confidence: 99%

The association of air pollutants (CO₂, MTBE) on Candida albicans and Candida glabrata drug resistance

Ghazanfari

Mohammadi

Rezaie

et al. 2022

Postępy Higieny I Medycyny Doświadczalnej

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Introduction Therapeutic methods are very important in the prevalence of opportunistic fungal infections, which are an important cause of human diseases. In this study, air pollution agents that are in direct contact with microorganisms, and the effects of carbon sources using CO2 and MTBE on growth of fungi, and particularly the evaluation of changes in the expression of interfering genes in susceptibility and drug resistance in these fungi, were investigated. Materials and Methods Collecting samples and isolating Candida glabrata and Candida albicans with phenotypic methods were accomplished. We then evaluated the minimum inhibitory concentration (MIC) with the M27A4 protocol of CLSI. We adjusted 20 strains of C. albicans and 10 strains of C. glabrata whose sensitivity was evaluated in the MIC test with 5% CO2 and 5mg/ml methyl tert-butyl ether (MTBE) considered as air pollutants, and followed by re-evaluating MIC testing to separate azole-resistant strains. Interfering agents were also considered. Results Upregulation of some genes on the two mentioned yeasts had led to drug resistance in them; they were previously sensitive to both drugs. Correspondingly, 41% of C. glabrata samples in sputum showed sensitivity to these drugs. Upregulation of ERG11 (71%) and EPA1 (90%) were observed in resistant strains. Upregulation of genes associated with aspartate proteins and downregulation of SAP3 genes were recognized in C. glabrata in sputum and a 15% downregulation of bronchoalveolar lavage (BAL) isolate and 50% upregulation of SAP1 gene in C. albicans sensitive samples were observed and compared to fluconazole and itraconazole with the oral and joint sources. Remarkably, decreased SAP2 expression in oral sources and a 60% increase in resistant strains in C. albicans were observed. The downregulation of SAP3 expression showed in the joint samples. An increase in HWP1 expression (30%) was noted in isolated and drug-sensitive samples at the sputum and BAL source. CDR1 expression was increased in MTBE-affected species; however, it decreased in the vicinity of CT. Conclusions Air pollutants such as CO2 and MTBE eventually caused drug resistance in Candida, which can be one of the causes of drug resistance in candidiasis infections.

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“…According to stakeholder theory, how companies choose to address sustainability concerns can be understood as a response to 'demands' from stakeholders (Freeman, 2010;Wheeler et al, 2003). In addition to creating shareholder value (Friedman, 1970), the social responsibility of business becomes creating stakeholder value (Freeman et al, 2004).…”

Section: How Stakeholders and Value Chain Organization Influence Sumentioning

confidence: 99%

Sustainability strategies by companies in the global coffee sector

Bager

Lambin

2020

Bus Strat Env

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The coffee sector is facing several sustainability challenges. We ask whether addressing these is transforming the entire coffee sector or rather leading to market differentiation. Drawing on stakeholder theory and global value chain analysis, we analyse how the coffee sector approaches sustainability by examining the sustainability efforts of a random sample of 513 companies. We also identify the factors shaping the adoption of sustainability strategies. A third of companies report no commitment to sustainability, whereas another third report vague commitment. The final third of companies report tangible commitments to sustainability. Company characteristics and stakeholders affect the scope and type of sustainability strategy chosen. Large, risk-aware companies tend to conduct 'hands-on' governance, adopting internal sustainability practices along their value chain. Small, consumerfacing companies and producers rely on 'hands-off' governance, adopting external voluntary sustainability standards. Several sustainability issues remain underaddressed by most companies, including climate change and deforestation. We found indications of potential greenwashing by some companies. Addressing sustainability is not yet fully mainstreamed in the sector, though ambitious commitments by sustainability leaders and large actors signal increasing importance of sustainability as part of corporate social responsibility efforts. We observe market differentiation through sustainability with progressive companies adopting sustainability strategies that align with their stakeholders, depending on value chain characteristics. Our results indicate a notable reliance on internal sustainability practices. There is a need for common coffee sustainability indicators relevant for all actors along the value chain, which are consistent with the Sustainable Development Goals, and a transparent, mandatory reporting framework.

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Genomic Analysis of Propane Metabolism in Methyl Tert-Butyl Ether-Degrading Mycobacterium Sp. Strain ENV421

Cited by 12 publications

References 30 publications

Distinct Catalytic Behaviors between Two 1,4-Dioxane-Degrading Monooxygenases: Kinetics, Inhibition, and Substrate Range

Distinct Catalytic Behaviors between Two 1,4-Dioxane-Degrading Monooxygenases: Kinetics, Inhibition, and Substrate Range

The association of air pollutants (CO₂, MTBE) on Candida albicans and Candida glabrata drug resistance

Sustainability strategies by companies in the global coffee sector

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Genomic Analysis of Propane Metabolism in Methyl Tert-Butyl Ether-Degrading Mycobacterium Sp. Strain ENV421

Cited by 12 publications

References 30 publications

Distinct Catalytic Behaviors between Two 1,4-Dioxane-Degrading Monooxygenases: Kinetics, Inhibition, and Substrate Range

Distinct Catalytic Behaviors between Two 1,4-Dioxane-Degrading Monooxygenases: Kinetics, Inhibition, and Substrate Range

The association of air pollutants (CO2, MTBE) on Candida albicans and Candida glabrata drug resistance

Sustainability strategies by companies in the global coffee sector

Contact Info

Product

Resources

About

The association of air pollutants (CO₂, MTBE) on Candida albicans and Candida glabrata drug resistance