Genomic and antigenic relationships between two ‘HoBi’-like strains and other members of the Pestivirus genus

Abstract

'HoBi'-like viruses comprise a putative new species within the genus Pestivirus of the family Flaviviridae. 'HoBi'-like viruses have been detected worldwide in batches of fetal calf serum, in surveillance programs for bovine pestiviruses and from animals presenting clinical signs resembling bovine viral diarrhea virus (BVDV)-associated diseases. To date, few complete genome sequences of 'HoBi'-like viruses are available in public databases. Moreover, detailed analyses of such genomes are still scarce. In an attempt to expand data on the genetic diversity and biology of pestiviruses, two genomes of 'HoBi'-like viruses recovered from Brazilian cattle were described and characterized in this study. Analysis of the whole genome and antigenic properties of these two new 'HoBi'-like isolates suggest that these viruses are genetically close to recognized pestiviruses. The present data provide evidence that 'HoBi'-like viruses are members of the genus Pestivirus and should be formally recognized as a novel species.

Genetic identification of pestiviruses from beef cattle in Southern Brazil

“…Weber et al [15] identified HoBiPeV associated with an outbreak of a gastro enteric condition resembling mucosal disease (MD). Mosena et al [25,34] reported the complete genome sequence of a BVDV-1i isolated from a cow in Southern Brazil and the complete genomic sequences of two Brazilian HoBiPeV isolates, respectively. Bianchi et al [29] described the isolation and genetic identification of 22 strains involved in an unusual outbreak of mucosal disease (MD).…”

“…HoBiPeV were initially identified as contaminants of fetal bovine serum (FBS) of Brazilian origin [13] and were subsequently linked to several livestock diseases in South America [14,15], Italy [16][17][18], and Asian countries [19][20][21]. BVDV-1 and BVDV-2 subgenotypes have been shown to circulate in Brazilian cattle in varied proportions, yet most genetic studies have been based on a single or few isolates or have included samples scattered over time and from different geographical origins or clinical backgrounds [7,14,[22][23][24][25][26][27][28]. The first evidence of circulation of HoBiPeV in Brazil dates from around 2002 and a number of subsequent studies indicate that these viruses are endemic among Brazilian cattle [7,14,15,24,25,27,[29][30][31].…”

A genetic profile of bovine pestiviruses circulating in Brazil (1998–2018)

“…Phylogenetic analyses based on the whole genome, 5 ′ UTR, and N pro sequences showed that strain was closely related to previously characterized BVDV-1i (JQ920104.1, JQ920215.1, and FJ493484.1) from the UK (90.5% highest nt identity at 5 ′ UTR) and Uruguay (KT833795.1) (90.6 high identity at N pro ). Mósena et al (2017aMósena et al ( , 2017b performed the full genomic sequencing and antigenic characterization of two previously described Brazilian HoBiPeV isolates (Bianchi et al, 2011;Weber et al, 2016aWeber et al, , 2016b. According to the authors, the presented data provided evidence that HoBiPeVs are members of the genus Pestivirus and should be formally recognized as a novel species.…”

“…In addition to D32/00_'HoBi' (Schirrmeier et al, 2004), at least four full genomic sequences of pestiviruses isolated in Brazil have been published: three HoBiPeV and one BVDV-1i. Mósena et al (2017aMósena et al ( , 2017b performed a full genomic sequencing and characterization of a BVDV-1i isolated from cattle in RS state. BVDV-1i is an uncommon subgenotype and has been detected in few regions (Yeşilbağ et al, 2017).…”

“…BVDV-1 and BVDV-2 have the broadest global distribution and have been associated with a variety of clinical manifestations in cattle (Houe, 2003) and a wide range of ruminant species, both domestic and free ranging. HoBiPeV were initially identified as contaminants of fetal bovine serum (FBS) of Brazilian origin (Schirrmeier et al ., 2004) and subsequently associated with a variety of clinical manifestations in cattle in South America (Cortez et al ., 2006; Decaro et al ., 2011; Bauermann et al ., 2013; Marques et al ., 2016; Weber et al ., 2016 a , 2016 b ; Mósena et al ., 2017 a , 2017 b ; Silveira et al ., 2017), Italy (Decaro et al ., 2011), Thailand (Liu et al ., 2009 a , 2009 b ), India (Mishra et al ., 2014), and Bangladesh (Haider et al ., 2014). It has also been isolated from water buffalo.…”