Genomic and biological characterization of chiltepín yellow mosaic virus, a new tymovirus infecting Capsicum annuum var. aviculare in Mexico

Pagán, Israel; Betancourt, Mónica; Miguel, Jacinto De; Fraile, Aurora; García‐Arenal, Fernando

doi:10.1007/s00705-010-0639-7

Cited by 20 publications

(13 citation statements)

References 49 publications

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“…A subset of 1081 plants, either symptomatic or asymptomatic, was analyzed for infection by ChYMV (a chiltepin-infecting tymovirus, see [36]), CMV, begomoviruses or potyviruses. Low prevalence of potyvirus infection (2.87%) precluded further analyses.…”

Section: Resultsmentioning

confidence: 99%

“…Infection by Chiltepin yellow mottle virus (ChYMV, Tymoviridae ) was analyzed by molecular hybridization using a 32 P-labeled RNA probe complementary to nucleotides 5365–5777 of ChYMV genomic RNA (Accession No. FN563124) [36]. Infection by species of the genus Begomovirus was detected by PCR using degenerate primers designed on the alignment of DNA-A sequences of 43 begomovirus species from the New World: BAOPsp (5′-GCGCCCTGCAGGGGCCYATGTAYAGGAAGCC-3′) and BAONsp (5′-GCGCGCGGCCGCGANGCATGNGTACATGCCAT-3′), which amplify a region in the coat protein gene located between nucleotide positions 392 and 884 in the genome of PepGMV (Accession No.…”

Section: Methodsmentioning

confidence: 99%

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Effect of Biodiversity Changes in Disease Risk: Exploring Disease Emergence in a Plant-Virus System

et al. 2012

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The effect of biodiversity on the ability of parasites to infect their host and cause disease (i.e. disease risk) is a major question in pathology, which is central to understand the emergence of infectious diseases, and to develop strategies for their management. Two hypotheses, which can be considered as extremes of a continuum, relate biodiversity to disease risk: One states that biodiversity is positively correlated with disease risk (Amplification Effect), and the second predicts a negative correlation between biodiversity and disease risk (Dilution Effect). Which of them applies better to different host-parasite systems is still a source of debate, due to limited experimental or empirical data. This is especially the case for viral diseases of plants. To address this subject, we have monitored for three years the prevalence of several viruses, and virus-associated symptoms, in populations of wild pepper (chiltepin) under different levels of human management. For each population, we also measured the habitat species diversity, host plant genetic diversity and host plant density. Results indicate that disease and infection risk increased with the level of human management, which was associated with decreased species diversity and host genetic diversity, and with increased host plant density. Importantly, species diversity of the habitat was the primary predictor of disease risk for wild chiltepin populations. This changed in managed populations where host genetic diversity was the primary predictor. Host density was generally a poorer predictor of disease and infection risk. These results support the dilution effect hypothesis, and underline the relevance of different ecological factors in determining disease/infection risk in host plant populations under different levels of anthropic influence. These results are relevant for managing plant diseases and for establishing conservation policies for endangered plant species.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Effect of Biodiversity Changes in Disease Risk: Exploring Disease Emergence in a Plant-Virus System

et al. 2012

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“…Viral infections in piquin pepper include Chiltepin yellow mosaic virus, which induce severe symptoms and cause a significant reduction in plant growth; Pepper golden mosaic virus that causes leaf wrinkles and reduced growth plant. Pepper huasteco yellow vein virus has a two-stranded genome (DNA-A and DNA-B) from single-stranded DNA and causes leaf deformation (Pag an et al, 2010;Renteria-Canett et al, 2011;Rodelo-Urrego et al, 2015). Human management of wild piquin pepper population has resulted in an increase of virus infection (Fraile et al, 2017).…”

Section: Environmental Conditionsmentioning

confidence: 99%

“…Wild pepper is considered an important genetic resource, as it has been reported with resistance to several viral groups including Tobamovirus, Potyviridae, Geminiviridae, and Bell pepper endornavirus (BPEV). Knowledge of pests and diseases helps in the development of strategies to anticipate, control, and understand the ecological role of population dynamics and genetic diversity (Okada et al, 2011;Pag an et al, 2010;Rodelo-Urrego et al, 2015).…”

Section: Environmental Conditionsmentioning

confidence: 99%

Horticultural Aspects for the Cultivated Production of Piquin Peppers (Capsicum annuum L. var. glabriusculum)—A Review

Mares-Quiñones¹,

Valiente-Banuet²

2019

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Piquin pepper [Capsicum annuum L. var. glabriusculum (Dunal) Heiser and Pickergill] is a semidomesticated pepper with high commercial value and wide applications as fresh or processed products. Piquin pepper plants have been difficult to domesticate and cultivate because of low seed germination, genetic and morphologic variability, insect and disease susceptibility, and limited environmental physiology information. Currently, seed sterility is no longer considered a limiting factor as hormonal, chemical, and thermal treatments have been developed to overcome seed dormancy. In vitro propagation (primarily by direct organogenesis) is still not reliable for seedling production. Cropping systems of piquin pepper plants include traditional methods such as agroforestry and full sunlight, and under protected horticulture conditions, mainly shade nets. Shade levels and water availability affect yield and vegetative growth. Piquin pepper plants can be grown under diverse geographic and edaphic conditions. Nutrition and fertilization studies are limited. Biotic stresses that can cause economic damage to piquin pepper plants include most that affect other pepper cultivars. Piquin pepper is also considered an important genetic resource as it reports resistance to some viral groups, which could be used for genetic improvement of other cultivated peppers. Current research needs involve the development of dependable plant materials (cultivated varieties) with reduced labor needs, particularly during the harvest period. In addition, research is needed to reduce the susceptibility of piquin pepper plants to other plant diseases. This review presents an analysis of the aspects related to the production of piquin peppers under cultivated conditions.

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“…Existem vinte e três espécies definitivas classificadas no gênero Tymovirus, e duas espécies tentativas (Haenni e Dreher, 2008). Os vírus do gênero Tymovirus têm sido descritos em espécies botânicas importantes para a agricultura, pertencentes principalmente às famílias Cucurbitaceae, Brassicaceae e Solanaceae (Pagán et al, 2010). Eles possuem distribuição mundial, amplo círculo de hospedeiras, ocorrem em alta concentração, nas plantas infectadas e podem ser transmitidos mecanicamente, por besouros crisomelídeos e, eventualmente, por sementes (Gibbs e Harrison, 1973;Martelli et al, 2002;Dreher et al, 2005;Colariccio et al, 2008).…”

Section: Introductionunclassified

Caracterização parcial de um Tymovirus isolado de tomateiros

et al. 2012

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Um Tymovirus isolado de sementes de tomateiro (Solanum lycopersicum) 'Paronset' com sintomas de necrose, denominado TyToRS08, foi caracterizado quanto ao círculo de hospedeiras, propriedades físico-químicas, sorológicas e moleculares. O vírus foi transmitido mecanicamente a partir de folhas, frutos e sementes infectadas. A gama de hospedeiras incluiu espécies de Amaranthaceae, Chenopodiaceae e Solanaceae, induzindo sintomas local e sistêmicos na maior parte das espécies, exceto para Nicotiana sylvestris, N. tabacum 'Samsun', 'White Burley' e 'Xanthi' que não manifestaram sintomas. Em tomateiros 'Alambra', 'Thomas', 'Diva', 'Romana' e 'Graziela', o vírus induziu sintomas de mosaico e necrose branca. A presença do vírus foi detectada, nas sementes infectadas de todas as cultivares avaliadas, porém não se notou em plântulas após a germinação destas sementes. Foi obtida uma preparação purificada com concentração de 1,04 mg mL-1, empregada na produção de um antissoro policlonal que reagiu em PTA-ELISA com um título de 1/32768. Utilizou-se um par de oligonucleotídeos degenerados, desenhados para anelar na ORF 1 de espécies de Tymovirus, que permitiu a amplificação por RT-PCR de fragmentos com cerca de 700 pb. Após o alinhamento e a análise das sequências, verificou-se que o isolado TyToRS08 possuía valores abaixo de 70% de identidade com as espécies de Tymovirus, indicando que pode se tratar de uma espécie distinta das descritas no gênero, com potencial epidemiológico, pela sua estabilidade e disseminação por sementes de tomate.

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Genomic and biological characterization of chiltepín yellow mosaic virus, a new tymovirus infecting Capsicum annuum var. aviculare in Mexico

Cited by 20 publications

References 49 publications

Effect of Biodiversity Changes in Disease Risk: Exploring Disease Emergence in a Plant-Virus System

Effect of Biodiversity Changes in Disease Risk: Exploring Disease Emergence in a Plant-Virus System

Horticultural Aspects for the Cultivated Production of Piquin Peppers (Capsicum annuum L. var. glabriusculum)—A Review

Caracterização parcial de um Tymovirus isolado de tomateiros

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