Genomic and biological characterization of a novel partitivirus infecting Fusarium equiseti

Mahillon, Mathieu; Decroës, Alain; Caulier, Simon; Tiendrebeogo, Assiata; Legrève, Anne; Bragard, Claude

doi:10.1016/j.virusres.2021.198386

Cited by 15 publications

(10 citation statements)

References 64 publications

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“…F. graminearum strains infected with Fusarium graminearum virus-ch9 (Fgv-ch9) [ 58 ] exhibit a reduced rate of mycelial growth and conidial production, abnormal colony morphology, and disorganized cytoplasm, as well as reduced virulence. Fusarium equiseti partitivirus 1 (FePV1) has been reported to confer hypovirulence to its host, Fusarium equiseti , including a lower growth rate, biomass production, and virulence on tomato [ 16 ]. Fusarium oxysporum f. sp.…”

Section: Discussionmentioning

confidence: 99%

“…At least forty-seven fully-sequenced mycoviruses have been identified in Fusarium , including Fusarium incarnatum alternavirus 1 (the proposed family Alternaviridae) [ 7 ], Fusarium oxysporum ourmia-like virus 1 ( Botourmiaviridae ) [ 8 ], Fusarium graminearum virus China 9 ( Chrysoviridae ) [ 9 ], Fusarium graminearum deltaflexivirus 1 ( Deltaflexiviridae ) [ 10 ], Fusarium poae dsRNA virus 3 (the proposed family Fusagraviridae) [ 11 ], Fusarium graminearum virus 1 ( Fusariviridae ) [ 12 ], Fusarium graminearum gemytripvirus 1 ( Genomoviridae ) [ 13 ], Fusarium pseudograminearum megabirnavirus 1 ( Megabirnaviridae ) [ 14 ], Fusarium boothii mitovirus 1 ( Mitoviridae ) [ 15 ], Fusarium equiseti partitivirus 1 ( Partitiviridae ) [ 16 ], Fusarium redolens polymycovirus 1 ( Polymycoviridae ) [ 17 ], Fusarium asiaticum victorivirus 1 [ 18 ] ( Totiviridae ), and others.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of the First Alternavirus Identified in Fusarium avenaceum, the Causal Agent of Potato Dry Rot

Zhang

Hua

et al. 2023

Viruses

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A novel virus with a double-stranded RNA (dsRNA) genome was isolated from Fusarium avenaceum strain GS-WW-224, the causal agent of potato dry rot. The virus has been designated as Fusarium avenaceum alternavirus 1 (FaAV1). Its genome consists of two dsRNA segments, 3538 bp (dsRNA1) and 2477 bp (dsRNA2) in length, encoding RNA-dependent RNA polymerase (RdRp) and a hypothetical protein (HP), respectively. The virions of FaAV1 are isometric spherical and approximately 30 nm in diameter. Multiple sequence alignments and phylogenetic analyses based on the amino acid sequences of RdRp and HP indicated that FaAV1 appears to be a new member of the proposed family Alternaviridae. No significant differences in colony morphology and spore production were observed between strains GS-WW-224 and GS-WW-224-VF, the latter strain being one in which FaAV1 was eliminated from strain GS-WW-224. Notably, however, the dry weight of mycelial biomass of GS-WW-224 was higher than that of mycelial biomass of GS-WW-224-VF. The depth and the width of lesions on potato tubers caused by GS-WW-224 were significantly greater, relative to GS-WW-224-VF, suggesting that FaAV1 confers hypervirulence to its host, F. avenaceum. Moreover, FaAV1 was successfully transmitted horizontally from GS-WW-224 to ten other species of Fusarium, and purified virions of FaAV1 were capable of transfecting wounded hyphae of the ten species of Fusarium. This is the first report of an alternavirus infecting F. avenaceum and conferring hypervirulence.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of the First Alternavirus Identified in Fusarium avenaceum, the Causal Agent of Potato Dry Rot

Zhang

Hua

et al. 2023

Viruses

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“…Sectoring has been reported as a common symptom caused by mycoviruses due to alteration of physiological and biochemical processes in fungi [27]. High-density sectors have been described for other fungi in relation to mycovirus infection, e.g., Beauveria bassiana [62], Colletotrichum fructicola [57], Macrophomina phaseolina [56], or Fusarium equiseti [63], and they have also been mentioned in the context of decreasing virus titer [64]. In contrast to other studies, the present work used genetically diverse fungal isolates (Supplementary Table S4), and we assume that different strains may respond differently to a viral invasion, which is probably represented in varying degrees of debilitation.…”

Section: Discussionmentioning

confidence: 99%

A New Double-Stranded RNA Mycovirus in Cryphonectria naterciae Is Able to Cross the Species Barrier and Is Deleterious to a New Host

Cornejo

Hisano

Bragança³

et al. 2021

JoF

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Cryphonectria is a fungal genus associated with economically significant disease of trees. Herein we characterized a novel double-stranded RNA virus from the fungal species Cryphonectria naterciae, a species unexplored as a virus host. De novo assembly of RNA-seq data and Sanger sequencing of RACE (rapid amplification of cDNA ends) clones gave the complete, non-segmented genome (10,164 bp) of the virus termed Cryphonectria naterciae fusagravirus (CnFGV1) that was phylogenetically placed within the previously proposed viral family Fusagraviridae. Of 31 field-collected strains of C. naterciae, 40% tested CnFGV1-positive. Cocultivation resulted in within-species transmission of CnFGV1 to virus-free strains of C. naterciae. Comparison of the mycelium phenotype and the growth rate of CnFGV1-infected and virus-free isogenic strains revealed frequent sectoring and growth reduction in C. naterciae upon virus infection. Co-culturing also led to cross-species transmission of CnFGV1 to Cryphonectria carpinicola and Cryphonectria radicalis, but not to Cryphonectria parasitica. The virus-infected C. naterciae and the experimentally infected Cryphonectria spp. readily transmitted CnFGV1 through asexual spores to the next generation. CnFGV1 strongly reduced conidiation and in some cases vegetative growth of C. carpinicola, which is involved in the European hornbeam disease. This study is the first report of a fusagravirus in the family Cryphonectriaceae and lays the groundwork for assessing a hypovirulence effect of CnFGV1 against the hornbeam decline in Europe.

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“…Sectoring has been reported as a common symptom caused by mycoviruses due to alteration of physiological and biochemical processes in fungi [27]. High-density sectors have been described for other fungi in relation with mycovirus infection, e.g., Beauveria bassiana [62], Colletotrichum fructicola [57], Macrophomina phaseolina [56], or Fusarium equiseti [63], and have also been mentioned in the context of decreasing virus titre [64]. In contrast to other studies, the present work used genetically diverse fungal isolates (Supplementary Table S5), and we assume that different strains may respond differently to a viral invasion, which probably is represented in varying degree of debilitation.…”

Section: Discussionmentioning

confidence: 99%

A New Double-Stranded RNA Mycovirus in <em>Cryphonectria naterciae</em> is Able to Cross the Species Barrier and is Deleterious to a New Host

Cornejo¹,

Hisano²,

Bragança³

et al. 2021

Preprint

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Cryphonectria is a fungal genus associated with economically significant disease of trees. Herein we characterized a novel double-stranded RNA virus from the fungal species Cryphonectria naterciae, a species unexplored as a virus host. De novo assembly of RNA-seq data and Sanger sequencing of RACE (rapid amplification of cDNA ends) clones gave the complete, non-segmented genome (10,164 bp) of the virus termed Cryphonectria naterciae fusagravirus (CnFGV1) that was phylogenetically placed within the previously proposed viral family Fusagraviridae. Of 31 field-collected strains of C. naterciae, 40% tested CnFGV1-positive. Co-cultivation resulted in within-species transmission of CnFGV1 to virus-free strains of C. naterciae. Comparison of the mycelium phenotype and the growth rate of CnFGV1-infected and virus-free isogenic strains revealed frequent sectoring and growth reduction in C. naterciae. Co-culturing also led to cross-species transmission of CnFGV1 to Cryphonectria carpinicola and Cryphonectria radicalis, but not to Cryphonectria parasitica. The virus-infected C. naterciae and the experimentally infected Cryphonectria spp. readily transmitted CnFGV1 through asexual spores to the next generation. CnFGV1 strongly reduced conidiation and in some cases vegetative growth of C. carpinicola, which is involved in the European hornbeam disease. This study is the first report of a fusagravirus in the family Cryphonectriaceae and lays the groundwork for assessing a hypovirulence effect of CnFGV1 against the hornbeam decline in Europe.

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Genomic and biological characterization of a novel partitivirus infecting Fusarium equiseti

Cited by 15 publications

References 64 publications

Characterization of the First Alternavirus Identified in Fusarium avenaceum, the Causal Agent of Potato Dry Rot

Characterization of the First Alternavirus Identified in Fusarium avenaceum, the Causal Agent of Potato Dry Rot

A New Double-Stranded RNA Mycovirus in Cryphonectria naterciae Is Able to Cross the Species Barrier and Is Deleterious to a New Host

A New Double-Stranded RNA Mycovirus in <em>Cryphonectria naterciae</em> is Able to Cross the Species Barrier and is Deleterious to a New Host

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