Genomic and functional potential of the immobilized microbial consortium MCSt-1 for wastewater treatment

Jha, Varsha; Dafale, Nishant A.; Hathi, Zubeen; Purohit, Hemant J.

doi:10.1016/j.scitotenv.2021.146110

Cited by 21 publications

(4 citation statements)

References 44 publications

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“…Specifically, the GlnA gene is involved in the conversion of ammonium nitrogen to L-Gln, while the GltB gene participates in the conversion of L-Gln to L-Glu [ 22 ]. Both L-Gln and L-Glu serve as essential energy sources for cellular biosynthesis and metabolism [ 32 ].…”

Section: Resultsmentioning

confidence: 99%

Denitrification Characteristics of the Low-Temperature Tolerant Denitrification Strain Achromobacter spiritinus HS2 and Its Application

Gao,

Zhang,

et al. 2024

Microorganisms

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The low-temperature environment significantly inhibits the growth and metabolism of denitrifying bacteria, leading to an excessive concentration of ammonia nitrogen and total nitrogen in sewage treatment plants during the cold season. In this study, an efficient denitrifying strain of heterotrophic nitrification–aerobic denitrification (HN–AD) bacteria named HS2 was isolated and screened from industrial sewage of a chemical factory in Inner Mongolia at 8 °C. The strain was confirmed to be Achromobacter spiritinus, a colorless rod-shaped bacterium. When cultured with sodium succinate as the carbon source, a carbon-to-nitrogen ratio of 20–30, a shaking rate of 150–180 r/min, and an initial pH of 6–10, the strain HS2 exhibited excellent nitrogen removal at 8 °C. Through the results of whole-genome sequencing, gene amplification, and gas product detection, the strain HS2 was determined to possess key enzyme genes in both nitrification and denitrification pathways, suggesting a HN–AD pathway of NH4+-N → NH2OH → NO2−N → NO → N2O → N2. At 8 °C, the strain HS2 could completely remove ammonia nitrogen from industrial sewage with an initial concentration of 127.23 mg/L. Microbial species diversity analysis of the final sewage confirmed Achromobacter sp. as the dominant genus, which indicated that the low-temperature denitrifying strain HS2 plays an important role in nitrogen removal in actual low-temperature sewage.

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Section: Resultsmentioning

confidence: 99%

Denitrification Characteristics of the Low-Temperature Tolerant Denitrification Strain Achromobacter spiritinus HS2 and Its Application

Gao,

Zhang,

et al. 2024

Microorganisms

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show abstract

“…Specifically, the GlnA gene is involved in the conversion of ammonium nitrogen to L-Gln, while the GltB gene participates in the conversion of L-Gln to L-Glu [21]. Both L-Gln and L-Glu serve as essential energy sources for cellular biosynthesis and metabolism [31].…”

Section: The Nitrogen Removal Pathway Of Strain Hs2mentioning

confidence: 99%

Denitrification Characteristics of the Low-Temperature Tolerant Denitrification Strain <em>Achromobacter spiritinus</em> HS2 and Its Application

Gao,

Zhang,

et al. 2024

Preprint

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The low-temperature environment significantly inhibits the growth and metabolism of denitrifying bacteria, leading to an excessive concentration of ammonia nitrogen and total nitrogen in sewage treatment plants during the cold season. In this study, an efficient denitrifying strain of heterotrophic nitrification–aerobic denitrification(HN-AD) bacteria named HS2 was isolated and screened from industrial sewage of a chemical factory in Inner Mongolia at 8°C. The strain was confirmed to be Achromobacter spiritinus, a colorless rod-shaped bacterium. When cultured with sodium succinate as the carbon source, a carbon-to-nitrogen ratio of 20-30, a shaking rate of 150-180 r/min, and the initial pH of 6-10, the Strain HS2 exhibited excellent nitrogen removal at 8°C. Through the results of whole-genome sequencing, gene amplification, and gas product detection, the Strain HS2 was determined to possess key enzyme genes in both nitrification and denitrification pathways, suggesting a HN-AD pathway of NH4+-N→NH2OH→NO2--N→NO→N2O→N2. At 8°C, the Strain HS2 could completely remove ammonia nitrogen from industrial sewage with an initial concentration of 127.23 mg/L. Microbial species diversity analysis of the final sewage confirmed Achromobacter sp. as the dominant genus, this indicated that the low-temperature denitrifying strain HS2 plays an important role in nitrogen removal of actual low-temperature sewage.

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“…Submerged plants are planted in the submerged oxygen pond, which can use the photosynthesis and respiration of submerged plants to build an ecological food chain system and realize the deep purification of water quality. Pebbles are preferably paved in the multi-stage falling water oxygenation ecological ditch, and they lay in a single ecological ditch facilitate the propagation and growth of biofilm on pebbles and promote the occurrence of nitrification reaction [25][26][27]. Aquatic plants are planted at the junction of the two ecological ditches and where the water flow is slow to form a multi-functional water drop oxygenation and ecological ditch, strengthen the purification effect, beautify the environment and increase economic benefits.…”

Section: Removal Mechanism Analysismentioning

confidence: 99%

A Modular Coupling System for Efficient Treatment of Rural Domestic Wastewater

Tian¹,

Chang²,

Yao³

et al. 2023

Pol. J. Environ. Stud.

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Rural domestic sewage has become very serious, while only 17.4% of it is treated centrally or partially, which results in environmental pollution and waste of water resources. To solve this problem, a wastewater treatment system by modular coupling is proposed, and the processing module includes two or three of multi-stage ecological pond, multi-stage drop aeration ecological ditch and multi-stage drop dam. The analysis of three examples shows that the system has a good treatment effect on chemical oxygen demand (COD), total nitrogen (TN), ammonium nitrogen (NH 4 + -N) and total phosphorus (TP), and the removal ratios are 90.22%, 82.07%, 91.60%, 81.48%, respectively, when the flow rate is 3 m 3 /h. After 28 days of continuous operation, the treatment capacity decreases by about 10%, indicating that the system has good long-term treatment performance. The removal ratio decreases slightly under different flow rates, which shows its great adaptability in different hydraulic conditions. In practical application, the system can select different coupling systems according to rural terrain and wastewater quality, and it is suitable for the treatment of rural domestic sewage and agricultural non-point source sewage and the repairment of urban sewage and lake eutrophic water body.

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Genomic and functional potential of the immobilized microbial consortium MCSt-1 for wastewater treatment

Cited by 21 publications

References 44 publications

Denitrification Characteristics of the Low-Temperature Tolerant Denitrification Strain Achromobacter spiritinus HS2 and Its Application

Denitrification Characteristics of the Low-Temperature Tolerant Denitrification Strain Achromobacter spiritinus HS2 and Its Application

Denitrification Characteristics of the Low-Temperature Tolerant Denitrification Strain <em>Achromobacter spiritinus</em> HS2 and Its Application

A Modular Coupling System for Efficient Treatment of Rural Domestic Wastewater

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