Genomic and histopathological characteristics of Vibrio parahaemolyticus isolated from an acute hepatopancreatic necrosis disease outbreak in Pacific white shrimp (Penaeus vannamei) cultured in Korea

Han, Jee Eun; Choi, Seong‐Kyoon; Han, Se‐Hyeon; Lee, Seung Chan; Jeon, Hye Jin; Lee, Cho-Rong; Kim, Kyeong Yeon; Lee, Young Seo; Park, Seul Chan; Rhee, Gahngyoon; Park, Seon-Young; Kim, Jun‐Seob; Park, Song; Kim, Ji Hyung; Lee, Kyeong‐Jun

doi:10.1016/j.aquaculture.2020.735284

Cited by 32 publications

(12 citation statements)

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“…These results indicate that transfer of plasmids may occur less within strains and more among species, e.g., the 93% sequence similarity of a virulence-associated plasmid from a shrimp pathogen V. nigripulchritudo to a plasmid from the coral pathogen V. shilonii (Reynaud et al, 2008 ). Furthermore, the Korean V. parahaemolyticus strains harbored the Asian-type AHPND plasmid, which was almost identical to the AHPND-associated V. owensii plasmid pVOWZ2 previously identified in China (Han et al, 2020 ). Therefore, plasmids may play a crucial role in the transfer of genes required by vibrios to infect marine animals.…”

Section: Discussionsupporting

confidence: 58%

“…On the other hand, vibrios are frequently reported as the major opportunistic pathogens causing vibriosis in cultured aquatic animals (Ruwandeepika et al, 2012;Xue et al, 2020;de Souza Valente and Wan, 2021). In general, most bacterial members of the Harveyi clade, such as V. parahaemolyticus and V. harveyi, have long been implicated in vibriosis in shrimp aquaculture, e.g., V. parahaemolyticus is the causative agent of acute hepatopancreatic necrosis disease (AHPND) (Han et al, 2020), whereas V. harveyi is closely associated wtih luminescent vibriosis (Vandenberghe et al, 1999;Ruwandeepika et al, 2010;Wang et al, 2015). In contrast, less attention has been paid to V. alginolyticus, although this opportunistic aquatic pathogen is increasingly associated with outbreak of shrimp vibriosis (Liu et al, 2004;Ren et al, 2013;Abdul Hannan et al, 2019;Bachand et al, 2020;de Souza Valente and Wan, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Comparative Genomic Analysis of Seven Vibrio alginolyticus Strains Isolated From Shrimp Larviculture Water With Emphasis on Chitin Utilization

et al. 2022

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The opportunistic pathogen Vibrio alginolyticus is gaining attention because of its disease-causing risks to aquatic animals and humans. In this study, seven Vibrio strains isolated from different shrimp hatcheries in Southeast China were subjected to genome sequencing and subsequent comparative analysis to explore their intricate relationships with shrimp aquaculture. The seven isolates had an average nucleotide identity of ≥ 98.3% with other known V. alginolyticus strains. The species V. alginolyticus had an open pan-genome, with the addition of ≥ 161 novel genes following each new genome for seven isolates and 14 publicly available V. alginolyticus strains. The percentages of core genes of the seven strains were up to 83.1–87.5%, indicating highly conserved functions, such as chitin utilization. Further, a total of 14 core genes involved in the chitin degradation pathway were detected on the seven genomes with a single copy, 12 of which had undergone significant purifying selection (dN/dS < 1). Moreover, the seven strains could utilize chitin as the sole carbon-nitrogen source. In contrast, mobile genetic elements (MGEs) were identified in seven strains, including plasmids, prophages, and genomic islands, which mainly encoded accessory genes annotated as hypothetical proteins. The infection experiment showed that four of the seven strains might be pathogenic because the survival rates of Litopenaeus vannamei postlarvae were significantly reduced (P < 0.05) when compared to the control. However, no obvious correlation was noted between the number of putative virulence factors and toxic effects of the seven strains. Collectively, the persistence of V. alginolyticus in various aquatic environments may be attributed to its high genomic plasticity via the acquisition of novel genes by various MGEs. In view of the strong capability of chitin utilization by diverse vibrios, the timely removal of massive chitin-rich materials thoroughly in shrimp culture systems may be a key strategy to inhibit proliferation of vibrios and subsequent infection of shrimp. In addition, transcontinental transfer of potentially pathogenic V. alginolyticus strains should receive great attention to avoid vibriosis.

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Section: Discussionsupporting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Comparative Genomic Analysis of Seven Vibrio alginolyticus Strains Isolated From Shrimp Larviculture Water With Emphasis on Chitin Utilization

et al. 2022

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“…The acute hepatopancreatic necrosis disease (AHPND), formerly known as shrimp "early mortality syndrome" (EMS), is a newly emerging vibriosis that has caused severe mortality (up to 100%) and devastating economic effect in the global shrimp industry (Lightner et al, 2012;Joshi et al, 2014;Kongrueng et al, 2015;Boonsri et al, 2017). Infected shrimp show slow growth, an empty stomach and midgut, and severe atrophy of hepatopancreas (Joshi et al, 2014;Kongrueng et al, 2015;Sirikharin et al, 2015;Han et al, 2020). Originally, the etiological agent of AHPND has been reported to be Vibrio parahaemolyticus (VP AHPND ) (Tran et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Mechanism of the Potential Therapeutic Candidate Bacillus subtilis BSXE-1601 Against Shrimp Pathogenic Vibrios and Multifunctional Metabolites Biosynthetic Capability of the Strain as Predicted by Genome Analysis

Wang

Zhu

et al. 2020

Front. Microbiol.

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The global shrimp industry has suffered bacterial diseases caused mainly by Vibrio species. The typical vibriosis, acute hepatopancreatic necrosis disease (AHPND), has resulted in mass mortality and devastating economic losses. Thus, therapeutic strategies are highly needed to decrease the risk of vibriosis outbreaks. Herein, we initially identified that the growth of the causative agent of AHPND, Vibrio parahaemolyticus (VP AHPND) and other vibrios in Pacific white shrimp (Litopenaeus vannamei) was inhibited by a Bacillus subtilis strain BSXE-1601. The natural products amicoumacins A, B, and C were purified from the cell-free supernatant from the strain BSXE-1601, but only amicoumacin A was demonstrated to be responsible for this anti-Vibrio activity. Our discovery provided the first evidence that amicoumacin A was highly active against shrimp pathogens, including the representative strain VP AHPND. Furthermore, we elucidated the amicoumacin A biosynthetic gene cluster by whole genome sequencing of the B. subtilis strain BSXE-1601. In addition to amicoumacin A, the strain BSXE-1601 genome harbored other genes encoding bacillibactin, fengycin, surfactin, bacilysin, and subtilosin A, all of which have previously reported antagonistic activities against pathogenic strains. The whole-genome analysis provided unequivocal evidence in support of the huge potential of the strain BSXE-1601 to produce diverse biologically antagonistic natural products, which may facilitate further studies on the effective therapeutics for detrimental diseases in shrimp.

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“…In fact, it has caused economic losses of billions of dollars since 2010, when it was first detected in China and Vietnam [ 3 , 4 ]. Since its emergence, it has rapidly spread and cases have been reported in Malaysia (2010), Thailand (2011), Mexico (2013), Philippines (2014), South American Countries (2014–2016), Bangladesh (2017), the USA (2017), Taiwan Province of China (2018), South Korea (2019), and the Okinawa Prefecture of Japan (2020) [ 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Exploring the Effect of Functional Diets Containing Phytobiotic Compounds in Whiteleg Shrimp Health: Resistance to Acute Hepatopancreatic Necrotic Disease Caused by Vibrio parahaemolyticus

Hernández‐Cabanyero

Carrascosa

Jiménez³

et al. 2023

Animals

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Acute hepatopancreatic necrosis (AHPND) is an emerging severe disease caused by strains of Vibrio parahaemolyticus (VpAHPND) in whiteleg shrimp (Litopenaeus vannamei). Mitigating its negative impact, and at the same time minimizing antibiotics treatments, is the major challenge in shrimp aquaculture. A sustainable strategy could be to include immunostimulants in diet. Phytobiotics, harmless plant extracts with immunostimulatory and biocidal activities, are promising candidates. In this study, we evaluated the effectiveness of two diets (E and F) supplemented with phytobiotics (functional diets) in terms of protecting shrimp against AHPND. For this purpose, groups of animals were fed functional or control diets for 4 and 5 weeks and, subsequently, they were challenged with VpAHPND by immersion. We compared the mortality in infected groups and estimated the percentage of carriers by using a specific qPCR in hepatopancreas tissue. The results showed that mortality was significantly lower in the group fed functional diet E and, after a 5-week feeding schedule. This group also showed the lowest percentage of carriers. The pathological effects were also reduced with diet F. Thus, feeding shrimp with phytobiotic-enriched diets in critical periods will be highly beneficial because it increases the host’s resistance to AHPND pathology.

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Genomic and histopathological characteristics of Vibrio parahaemolyticus isolated from an acute hepatopancreatic necrosis disease outbreak in Pacific white shrimp (Penaeus vannamei) cultured in Korea

Cited by 32 publications

References 35 publications

Comparative Genomic Analysis of Seven Vibrio alginolyticus Strains Isolated From Shrimp Larviculture Water With Emphasis on Chitin Utilization

Comparative Genomic Analysis of Seven Vibrio alginolyticus Strains Isolated From Shrimp Larviculture Water With Emphasis on Chitin Utilization

Mechanism of the Potential Therapeutic Candidate Bacillus subtilis BSXE-1601 Against Shrimp Pathogenic Vibrios and Multifunctional Metabolites Biosynthetic Capability of the Strain as Predicted by Genome Analysis

Exploring the Effect of Functional Diets Containing Phytobiotic Compounds in Whiteleg Shrimp Health: Resistance to Acute Hepatopancreatic Necrotic Disease Caused by Vibrio parahaemolyticus

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