Genomic and Immunological Characterization of Hypermucoviscous Carbapenem-Resistant Klebsiella pneumoniae ST25 Isolates from Northwest Argentina

Albarracín, Leonardo; Moyano, Ramiro Ortiz; Vargas, Juan M. Casa; Andrade, Bruno de Bezerril; Zamar, Juan Cortez; Maidana, Stefanía Dentice; Fukuyama, Kohtaro; Kurata, Shoichiro; Jure, María Ángela; Kitazawa, Haruki; Villena, Julio

doi:10.3390/ijms23137361

Cited by 10 publications

(19 citation statements)

References 65 publications

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“…As in this study, these ST25 isolates showed a K2 capsular serotype linked to increased virulence [2], although no genomic data is available for comparison. Despite two draft genomes of isolates from Argentina proposed to be ST25 were published [51], our analyses indicated that they actually are ST629 and ST551-1LV.…”

Section: Discussionmentioning

confidence: 66%

Population genomics, resistance, pathogenic potential, and mobile genetic elements of carbapenem-resistantKlebsiella pneumoniaecausing infections in Chile

Veloso

Acosta

Arros

et al. 2022

Preprint

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Multidrug and carbapenem-resistant K. pneumoniae (CR-Kp) are considered critical threats to global health and key traffickers of resistance genes to other pathogens. In Chile, although a sustained increase in CR-Kp infections has been observed, few strains have been described at the genomic level, lacking molecular details of their resistance and virulence determinants and the mobile elements mediating their dissemination. In this work, we studied the antimicrobial resistance and performed a comparative genomics analysis of ten CR-Kp isolates from the Chilean surveillance of carbapenem-resistant Enterobacteriaceae. High resistance to most of the antibiotics tested was observed among the isolates, five ST25, three ST11, one ST45, and one ST505, which harbored a total of 44 plasmids, many of them predicted to be conjugative and carrying genes conferring resistance to a variety of antibiotic, metals, and disinfectants. Ten plasmids encoding either KPC-2, NDM-1, or NDM-7 carbapenemases were characterized, including novel plasmids with increased resistance gene load and a novel genetic environment for blaKPC-2 gene. This corresponds to the first report of ST25 and ST45 Kp producing NDM-7 in South America, and of an ST505 CR-Kp worldwide, producing both NDM-7 and KPC-2. Moreover, we characterized a variety of genomic islands carrying virulence and fitness factors. These results provide baseline knowledge for the detailed understanding of molecular and genetic determinants behind antibiotic resistance and virulence of K. pneumoniae in Chile and South America.

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Section: Discussionmentioning

confidence: 66%

Population genomics, resistance, pathogenic potential, and mobile genetic elements of carbapenem-resistantKlebsiella pneumoniaecausing infections in Chile

Veloso

Acosta

Arros

et al. 2022

Preprint

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“…In the current study, PCR-based molecular screening for K1 and K2 capsular serotypes revealed that 30% (15/50) of Kp isolates belonged to capsular serotype K2 (harboring wzy gene), with 86.7% (13/15) of the K2 isolates being hmvKp phenotypes, which represent 61.9% of all hmvKp isolates. None of the Kp isolates have been identified as the K1 capsular type attributable to the absence of the magA gene [ 53 , 54 ]. However, it was reported that the magA gene is confined to liver abscesses and linked to hypermucoviscosity and phagocytosis resistance [ 2 , 55 , 56 , 57 ].…”

Section: Discussionmentioning

confidence: 99%

Occurrence and Molecular Study of Hypermucoviscous/Hypervirulence Trait in Gut Commensal K. pneumoniae from Healthy Subjects

et al. 2023

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Hypervirulent Klebsiella pneumoniae (hvKp) is emerging worldwide. Hypermucoviscousity is the characteristic trait that distinguishes it from classic K. pneumoniae (cKp), which enables Kp to cause severe invasive infections. This research aimed to investigate the hypermucoviscous Kp (hmvKp) phenotype among gut commensal Kp isolated from healthy individuals and attempted to characterize the genes encoding virulence factors that may regulate the hypermucoviscosity trait. Using the string test, 50 identified Kp isolates from healthy individuals’ stool samples were examined for hypermucoviscosity and investigated by transmission electron microscopy (TEM). Antimicrobial susceptibility profiles of Kp isolates were determined using the Kirby Bauer disc method. Kp isolates were tested for genes encoding different virulence factors by PCR. Biofilm formation was assayed by the microtiter plate method. All Kp isolates were multidrug-resistant (MDR). Phenotypically, 42% of isolates were hmvKp. PCR-based genotypic testing revealed the hmvKp isolates belonged to capsular serotype K2. All study Kp isolates harbored more than one virulence gene. The genes magA and rmpA were not detected, while the terW gene was present in all isolates. The siderophores encoding genes entB and irp2 were most prevalent in hmvKp isolates (90.5%) and non-hmvKp (96.6%), respectively. hmvKp isolates harbored the genes wabG and uge with rates of 90.5% and 85.7%, respectively. The outcomes of this research highlight the potential health risk of commensal Kp to cause severe invasive diseases, owing to being hmvKp and MDR, and harboring multiple virulence genes. The absence of essential genes related to hypermucoviscosity such as magA and rmpA in hmvKp phenotypes suggests the multifactorial complexity of the hypermucoviscosity or hypervirulence traits. Thus, further studies are warranted to verify the hypermucoviscosity-related virulence factors among pathogenic and commensal Kp in different colonization niches.

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“…Above all, was shown that K. pneumoniae DY1928 had high dissemination and transfer ability. Animal models are one of the methods to identify the virulence of K. pneumoniae strains (Albarracin et al, 2022). In the mouse experiment, we isolated 11 K. pneumoniae strains that harbored bla NDM-1 homologous to DY1928.…”

Section: Discussionmentioning

confidence: 99%

“…However, to our knowledge, there are few studies on the transfer and colonization of CRKP from blood to the intestine in mice. Furthermore, there were no studies that characterized the virulence of K. pneumoniae ST25 strains in animal models except the study of Albarracin et al (2022 ). Our findings indicated the high virulence of strain DY1928, together with its resistance to multiple antimicrobial agents.…”

Section: Discussionmentioning

confidence: 99%

Emergence of a NDM-1-producing ST25 Klebsiella pneumoniae strain causing neonatal sepsis in China

Zhao

Zheng²,

Xu³

et al. 2022

Front. Microbiol.

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Carbapenem-resistant Klebsiella pneumoniae (CRKP) seriously threaten the efficacy of modern medicine with a high associated mortality rate and unprecedented transmission rate. In this study, we isolated a clinical K. pneumoniae strain DY1928 harboring blaNDM-1 from a neonate with blood infection. Antimicrobial susceptibility testing indicated that DY1928 was resistant to various antimicrobial agents, including meropenem, imipenem, ceftriaxone, cefotaxime, ceftazidime, cefepime, piperacillin-tazobactam, and amoxicillin-clavulanate. S1 nuclease-pulsed field gel electrophoresis (S1-PFGE), southern blot and conjugation experiment revealed that the blaNDM-1 gene was located on a conjugative plasmid of IncA/C2 type with a 147.9 kb length. Whole-genome sequencing showed that there was a conservative structure sequence (blaNDM-1-ble-trpF-dsbD) located downstream of the blaNDM-1 gene. Multilocus sequence typing (MLST) classified DY1928 as ST25, which was a hypervirulent K. pneumoniae type. Phylogenetic analysis of genomic data from all ST25 K. pneumoniae strains available in the NCBI database suggested that all blaNDM-1 positive strains were isolated in China and had clinical origins. A mouse bloodstream infection model was constructed to test the virulence of DY1928, and 11 K. pneumoniae strains homologous to DY1928 were isolated from the feces of infected mice. Moreover, we found that DY1928 had a tendency to flow from the blood into the intestine in mice and caused multiple organ damage. To our knowledge, this is the first study to report an infection caused by blaNDM-1-positive ST25 K. pneumoniae in the neonatal unit. Our findings indicated that stricter surveillance and more effective actions were needed to reduce the risk of disseminating such K. pneumoniae strains in clinical settings, especially in neonatal wards.

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Genomic and Immunological Characterization of Hypermucoviscous Carbapenem-Resistant Klebsiella pneumoniae ST25 Isolates from Northwest Argentina

Cited by 10 publications

References 65 publications

Population genomics, resistance, pathogenic potential, and mobile genetic elements of carbapenem-resistantKlebsiella pneumoniaecausing infections in Chile

Population genomics, resistance, pathogenic potential, and mobile genetic elements of carbapenem-resistantKlebsiella pneumoniaecausing infections in Chile

Occurrence and Molecular Study of Hypermucoviscous/Hypervirulence Trait in Gut Commensal K. pneumoniae from Healthy Subjects

Emergence of a NDM-1-producing ST25 Klebsiella pneumoniae strain causing neonatal sepsis in China

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