Genomic and phenotypic divergence among three serotypes of aquatic birnaviruses (infectious pancreatic necrosis virus)

Macdonald, Richard D.; Gower, David

doi:10.1016/0042-6822(81)90264-6

Cited by 60 publications

(37 citation statements)

References 25 publications

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“…110000, 67000 and 40000. In contrast, Macdonald & Gower (1981) found that all three viruses could be distinguished by either the number or mobilities of the virion polypeptides. They found that SP differed from the VR-299 IPNV strain by the absence of the cleavage product (VP4) of VP3.…”

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confidence: 63%

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Biochemical Characterization of Eel Virus European

Hedrick

Okamoto

Sano

et al. 1983

Journal of General Virology

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SUMMARYThe polypeptide and RNA compositions of purified virions of eel virus European were compared to those of three strains (VR-299, SP and AB) of infectious pancreatic necrosis virus (IPNV) isolated from trout. All three IPNV strains could be distinguished by the relative mobilities of either the virion polypeptides or the doublestranded RNA genome segments. The eel virus had a similar polypeptide profile to strain AB IPNV but differences between the two viruses in the migration of genome segments indicated each was unique. Annual epizootics among pond-cultured Japanese eels (Anguilla japonica) in Shizuoka prefecture, Japan have been reported since 1969 (Egusa, 1970). Environmental and physiological factors alone or in combination with a bacterium of the genus Cytophaga were believed to be the major causes of the observed epizootics (Egusa et al., 1971 ;Egusa, 1976). Virological examinations of diseased eels, however, revealed the presence of a previously undescribed virus (Sano, 1976). Examinations of eels from farms in 10 different locations indicated that this same virus was present in several populations of cultured eels in Japan (Sano et al., 1981). This disease outbreak followed the importation of European eels (Anguilla anguilla) to Shizuoka prefecture. The virus was also isolated from these imported eels. Because the virus showed an antigenic relationship to a European strain of infectious pancreatic necrosis virus (IPNV), it was designated eel virus European (EVE).Infectious pancreatic necrosis virus is a known pathogen of hatchery reared brook (Salvelinus fontinalis) and rainbow (Salmo gairdneri) trout and is found in many countries where these fish are cultured (Wolf, 1966). Three major serological groups or serotypes of IPNV have been identified (Okamoto et al., 1983;Macdonald & Gower, 1981). Two of these serotypes, AB and SP (Jorgensen & Kehlet, 1971), are found only in Europe. Both the AB and SP serotypes have been detected in trout, in several nonsalmonid species and in elvers in Europe (Hudson et al., 1981 ;Adair & Ferguson, 1981 ;Castric & Chastel, 1980; Munro et al., 1976; Ahne, 1980). The third serotype of IPNV is prevalent in North America and is represented by reference strain VR-299 (Wolf & Quimby, 1971).Eel virus European (EVE) is similar to IPNV in particle size and morphology, cytopathogenic effect in cell culture and buoyant density in caesium chloride (Nishimura et al., 1981). Unlike the VR-299 and SP strains, EVE was not pathogenic for rainbow trout even though the virus was found to replicate to high concentrations in several internal organs (Sano et aL, 1981). Similar results were obtained with the AB strain of IPNV which was less virulent for rainbow trout (Jorgensen & Kehlet, 1971) than SP or VR-299 IPNV. Cross-neutralization studies have shown EVE to be closely related to the AB strain of IPNV (Okamoto et al., 1983). The results presented here provide the first comparisons of the RNA and polypeptides of VR-299, SP and AB IPNV with EVE. From this study, we have confirmed that E...

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confidence: 63%

“…Three major serological groups or serotypes of IPNV have been identified (Okamoto et al, 1983;Macdonald & Gower, 1981). Two of these serotypes, AB and SP (Jorgensen & Kehlet, 1971), are found only in Europe.…”

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confidence: 99%

Biochemical Characterization of Eel Virus European

Hedrick

Okamoto

Sano

et al. 1983

Journal of General Virology

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“…Optimal yields are obtained at temperatures of 18 °C to 24 °C (Malsberger & Cerini, 1965;Kelly & Lob, 1975;La Bonnardiere et al, 1976;Dorson et al, 1978;Macdonald & Gower, 1981). This temperature range corresponds closely to the optimal temperature ranges for cell lines derived from salmonids.…”

Section: Introductionmentioning

confidence: 99%

Studies on the Mechanism of Temperature Sensitivity of Infectious Pancreatic Necrosis Virus Replication

Roberts

Dobos

1983

Journal of General Virology

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SUMMARYYields of infectious pancreatic necrosis virus from fathead minnow cell cultures were maximal at 20 °C. The virus failed to replicate at 28 °C and neither virus-specific mRNA nor virus-specific polypeptides could be detected when infected cells were maintained at this temperature. Intrinsic thermolability of virus infectivity or inability to adsorb to cells at 28 °C could not account for the temperature-dependent block in virus morphogenesis. Analysis of infectious virus production and virus-specific polypeptide and RNA synthesis following shifts from the permissive (20 °C) to the non-permissive temperature (28 °C) at various times after infection indicated that multiple temperature-sensitive (ts) steps were involved in the inhibition of virus replication.

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“…The first European isolates, Ab and SP, were shown to be antigenically different from the original reference strain, ATCC LWVRT 60-1 (VR-299) (J~rgensen & Keblet, 1971;Wolf & Quimby, 1971). Until 1983, only these three major serotypes were recognized (MacDonald & Gower, 1981;Okamoto et al, 1983). However, Hill & Way (1983, on the basis of cross-neutralization assays, have identified six additional serotypes and two serogroups.…”

Section: Introductionmentioning

confidence: 99%