Genomic and phenotypic diversity of Listeria monocytogenes clonal complexes associated with human listeriosis

Bergholz, Teresa M.; Shah, Manoj Kumar; Burall, Laurel S.; Rakić-Martinez, Mira; Datta, Atin R.

doi:10.1007/s00253-018-8852-5

Cited by 63 publications

(78 citation statements)

References 95 publications

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“…Canadian cases of listeriosis have been predominantly associated with CC8 L. monocytogenes (2,27). The present study indicates that CC8 L. monocytogenes are also frequently isolated from foods (Fig.…”

Section: Carriage Of Qac-resistance In Clinically-relevant Clonal Comsupporting

confidence: 61%

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Genomic Markers for Quaternary Ammonium Compound Resistance as a Persistence Indicator for Listeria monocytogenes Contamination in Food Manufacturing Environments

Cooper

Carrillo

Deschênes

et al. 2021

Journal of Food Protection

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Persistent contamination of food manufacturing environments by Listeria monocytogenes is an important public health risk because such contamination events defy standard sanitization protocols, for example, the application of quaternary ammonium compounds such as benzalkonium chloride (BC), providing a source for prolonged dissemination of the bacteria in food products. We performed whole-genome sequence (WGS) analyses of 1279 well-characterized L. monocytogenes isolates from a variety of foods and food manufacturing environments and identified the bcrABC gene cassette associated with BC resistance in 41.5% of isolates. Of particular interest was the finding that all but one of 177 clonal complex (CC) 321 isolates, representing one of the most commonly occurring CCs found in foods and food-production environments, harbored the intact bcrABC cassette. Thirty-nine (38.6) percent of isolates recovered from foods representing 67 different CCs, and 59.2% of strains from food-manufacturing environmental samples representing 26 different CCs, were found to harbor the intact bcrABC cassette. A representative set of 69 isolates with and without bcrABC was assayed for the ability to grow in the presence of BC, and 34 of 35 isolates harboring the bcrABC cassette were resistant to BC. Determination of bcrABC in colony isolates could be achieved using both polymerase chain reaction and whole genome sequencing techniques, providing food testing laboratories with options for the characterization of isolates. The ability to detect bcrABC provides risk managers with a valuable tool to assess the potential for persistent contamination of the food manufacturing environment, which in turn may indicate the need for more targeted surveillance to ensure the efficacy of mitigation actions.

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Section: Carriage Of Qac-resistance In Clinically-relevant Clonal Comsupporting

confidence: 61%

“…Following genomic sequence analyses, a novel mobile genetic element encoding the multi-drug resistant efflux pump encoded by emrE was postulated to be associated with persistence of outbreak-related strains (2,18).…”

Section: Highlightsmentioning

confidence: 99%

Genomic Markers for Quaternary Ammonium Compound Resistance as a Persistence Indicator for Listeria monocytogenes Contamination in Food Manufacturing Environments

Cooper

Carrillo

Deschênes

et al. 2021

Journal of Food Protection

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“…Even more concerning, an examination of WGS submissions to NCBI suggests the possibility that these strains are expanding as increasing numbers of 4bV strains have been identified; this may be due to increased sequencing efforts which facilitates screening for these strains, however (3,8). Surveillance of isolates obtained from clinical and food samples, as well as the natural environment, will further aid in understanding this emerging threat to public health.…”

Section: Doumith Et Al Developed a Multiplex Conventional Pcr Methodmentioning

confidence: 99%

Development and Validation of a Quantitative PCR Method for Species Verification and Serogroup Determination of Listeria monocytogenes Isolates

Burall

Sepehri

Srinivasan

et al. 2021

Journal of Food Protection

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Listeria monocytogenes (Lm) is one of the leading causes of death due to foodborne illness, affecting the elderly, pregnant women, neonates and the immune compromised. Serologically, Lm can be classified into 13 serotypes, though only four are typically linked with food contamination and illness. Since 2000, a shift in serotypes involved in listeriosis outbreaks was observed, suggesting that tracking of serotypes could help identify emerging trends. A PCR method developed in 2004 allowed detection of the four major serotypes as molecular serogroups, corresponding to broad phylogenetic groups. In this study, a novel qPCR method was developed that uses two multiplex qPCRs, one to confirm the Listeria genus and Lm species, and the second for Lm molecular serogrouping. This method was compared with the FDA Bacteriological Analytical Manual (BAM) method for Lm and the sero-agglutination method, using a 208-strain panel. Comparison of the genus/species qPCR assay with the BAM methods found an equal or slightly higher accuracy for the qPCR method (>98%), as compared to the BAM protocol (>96%), when evaluated against independent characterization data. Molecular serogrouping using the qPCR method (96.6%) was more accurate than the sero-agglutination assay (75.6%). The qPCR method identified Lm 4bV strains, which could not be resolved using sero-agglutination. The qPCR could not identify lineages III and IV serotype 4b strains but did correctly identify 16 of 18 lineage III/IV strains. The qPCR method performed genus identification for Lm, L. innocua, L. welshimeri, L. ivanovii, and L. seeligeri. Additionally, the method performed species identification for Lm, and serogrouped Lm into six molecular serogroups, 2A, 2B, 2C, 4B, NT, and 4bV. This method provided a rapid and accurate confirmation of Lm and serogroup determinations; furthermore, it could help identify otherwise unlinked strains by enabling WGS analysis based on broad phylogeny, independent of other information.

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“…The bacterium Listeria monocytogenes is ubiquitous, and able to survive and grow at a wide range of temperatures, and in alkaline or acid media and high osmolality conditions [1]. Most of these stressful conditions are common in food processing environments (FPE) and inside the human host during infection [2]. L. monocytogenes is exposed to acid and high osmolality within food matrices (e.g.…”

Section: Introductionmentioning

confidence: 99%

The Response to Oxidative Stress in Listeria monocytogenes Is Temperature Dependent

et al. 2020

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The stress response of 11 strains of Listeria monocytogenes to oxidative stress was studied. The strains included ST1, ST5, ST7, ST6, ST9, ST87, ST199 and ST321 and were isolated from diverse food processing environments (a meat factory, a dairy plant and a seafood company) and sample types (floor, wall, drain, boxes, food products and water machine). Isolates were exposed to two oxidizing agents: 13.8 mM cumene hydroperoxide (CHP) and 100 mM hydrogen peroxide (H2O2) at 10 °C and 37 °C. Temperature affected the oxidative stress response as cells treated at 10 °C survived better than those treated at 37 °C. H2O2 at 37 °C was the condition tested resulting in poorest L. monocytogenes survival. Strains belonging to STs of Lineage I (ST5, ST6, ST87, ST1) were more resistant to oxidative stress than those of Lineage II (ST7, ST9, ST199 and ST321), with the exception of ST7 that showed tolerance to H2O2 at 10 °C. Isolates of each ST5 and ST9 from different food industry origins showed differences in oxidative stress response. The gene expression of two relevant virulence (hly) and stress (clpC) genes was studied in representative isolates in the stressful conditions. hly and clpC were upregulated during oxidative stress at low temperature. Our results indicate that conditions prevalent in food industries may allow L. monocytogenes to develop survival strategies: these include activating molecular mechanisms based on cross protection that can promote virulence, possibly increasing the risk of virulent strains persisting in food processing plants.

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Genomic and phenotypic diversity of Listeria monocytogenes clonal complexes associated with human listeriosis

Cited by 63 publications

References 95 publications

Genomic Markers for Quaternary Ammonium Compound Resistance as a Persistence Indicator for Listeria monocytogenes Contamination in Food Manufacturing Environments

Genomic Markers for Quaternary Ammonium Compound Resistance as a Persistence Indicator for Listeria monocytogenes Contamination in Food Manufacturing Environments

Development and Validation of a Quantitative PCR Method for Species Verification and Serogroup Determination of Listeria monocytogenes Isolates

The Response to Oxidative Stress in Listeria monocytogenes Is Temperature Dependent

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