Genomic and physiological diversity amongst strains of Thiobacillus ferrooxidans, and genomic comparison with Thiobacillus thiooxidans

Harrison, Arthur P.

doi:10.1007/bf00451501

Cited by 125 publications

(81 citation statements)

References 34 publications

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“…The data on DNA2DNA hybridization also indicate high genotypic divergence of A. ferrooxidans strains, which, with the exception of six independent considerably divergent strains, constitute four groups characterized by a high level of genome similarity (genomovars). These data agree well with the earlier findings of Harrison (1982), who also revealed five groups of A. ferrooxidans strains corresponding to genomovars and several strains that could not be affiliated with any of these groups. The high level of DNA-DNA relatedness (96 %) of the genomes of strain ATCC 19859 and the type strain ATCC 23270 T suggests that all the allied strains studied by Harrison and us constitute one genomovar and therefore, that the members of this genomovar must be widespread in nature.…”

Section: Discussionsupporting

confidence: 93%

“…These data indicate a close relatedness of the species A. ferrooxidans and A. thiooxidans and allow the suggestion to be made that the latter species has diverged from the former species because of a gradual accumulation of phenotypic differences. According to the findings of Harrison (1982), A. thiooxidans strain DSM 612 does not belong to the same genomovar as other A. thiooxidans strains. Analysis of 16S rRNA gene sequences confirmed the incorrect taxonomic identification of this strain, as well as of some other strains such as KCTC 8929P and LM2 (the latter strain probably belongs to the species A. caldus).…”

Section: Discussionmentioning

confidence: 92%

“…The DNA2DNA hybridization analysis of A. ferrooxidans and A. thiooxidans strains carried out by Harrison (1982) showed low genome similarity (not more than 23 %). Moreover, most of the A. thiooxidans strains studied, including the type strain but excluding strain DSM 612, were found to belong to one genomovar.…”

Section: Discussionmentioning

confidence: 98%

“…Analysis of 16S rRNA gene sequences of the strains studied by DNA-DNA hybridization by Harrison (1982) allowed us, in addition, to compare the results yielded by the two methods, and showed that A. ferrooxidans strains ATCC 19859 and F221 from phylogenetic group I belong to one genomovar, while strains IFO 14262, from phylogenetic group II, and IFO 14245, from a separate phylogenetic branch, represent two other genomovars.…”

Section: Discussionmentioning

confidence: 99%

“…The habitats of A. ferrooxidans strains are geographically extremely diverse and vary in their physico-chemical conditions (presence of particular sulfide minerals and their ratio, pH, temperature and the content of toxic compounds in the liquid phase). This might explain the polymorphism of A. ferrooxidans strains, in terms of both their physiological properties (Tuovinen et al, 1971;Harrison, 1982;Valenti et al, 1989;Leduc & Ferroni, 1994;Chisholm et al, 1998;Frattini et al, 2000;Ageeva et al, 2001) and genotypic characteristics (Martin et al, 1981;Harrison, 1982;Shiratori et al, 1991;Kondratyeva et al, 1993Kondratyeva et al, , 1995Rawlings & Kusano, 1994;Rawlings, 1999). The study of the natural genotypic and phenotypic variability of bacterial strains is of great importance in discerning the microevolutionary mechanisms of species formation and for their monitoring in biohydrometallurgical processes and in nature.…”

Section: Introductionmentioning

confidence: 99%

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Phylogenetic heterogeneity of the species Acidithiobacillus ferrooxidans

Karavaiko

Turova

et al. 2003

International Journal of Systematic and Evolutionary Microbiology

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Polyphasic genotypic analysis of 25 Acidithiobacillus ferrooxidans strains isolated from ores and ore concentrates collected in different regions of the world showed considerable strain heterogeneity. Restriction patterns of the chromosomal DNA of these strains obtained by PFGE were specific for each strain. According to the degree of DNA relatedness, 17 of the 23 strains studied were divided into four genomovars. Six independent, considerably divergent strains could not be assigned to any of the genomovars. A comparison of nearly complete nucleotide sequences of the 16S rDNA of five representatives of the genomovars (including the type strain of A. ferrooxidans, ATCC 23270 T ) with those of species of the genus Acidithiobacillus available from GenBank showed that most of the A. ferrooxidans strains, together with the type strain and some other strains of the species Acidithiobacillus thiooxidans, comprised a monophyletic cluster. Within this major cluster, A. ferrooxidans strains fell into four phylogenetic groups that were equidistant from the phylogenetic group of A. thiooxidans strains. In general, the distribution of strains among the phylogenetic groups correlated with their distribution among the genomovars, except that the representatives of two different genomovars fell into one phylogenetic group. Thus, at least two levels of phylogenetic heterogeneity for A. ferrooxidans have been found. The phylogenetic heterogeneity of A. ferrooxidans strains, which are phenotypically indistinguishable, suggests the occurrence of microevolutionary processes in different econiches. This should be taken into account in the biohydrometallurgical applications of A. ferrooxidans strains.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phylogenetic heterogeneity of the species Acidithiobacillus ferrooxidans

Karavaiko

Turova

et al. 2003

International Journal of Systematic and Evolutionary Microbiology

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Thiobacillus

Kelly

Wood

Stackebrandt

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Thi.o.ba.cil' lus . Gr. n. thios sulfur; L n bacillus a small rod; M. L. masc n. Thiobacillus sulfur rodlet. Proteobacteria / Betaproteobacteria / Hydrogenophilales / Hydrogenophilaceae / Thiobacillus Small, Gram‐negative, rod‐shaped cells (0.3–0.5 × 0.9–4.0 µm). Some species are motile by means of polar flagella. No resting stages known. Energy is derived by the oxidation of one or more reduced sulfur compounds, including sulfides, sulfur, thiosulfate, polythionates, and thiocyanate. Sulfate is the end product of sulfur‐compound oxidation, but sulfur, sulfite, and polythionates may be accumulated by most species, sometimes transiently. All species can fix carbon dioxide by means of the Benson–Calvin cycle and are capable of autotrophic growth; some species are obligately chemolithotrophic, while others are chemoorganotrophic. The genus currently includes obligate aerobes and facultative denitrifiers. Optimal pH of 2–8 with optimal temperature of 28–43°C. Distribution is seemingly ubiquitous in marine, freshwater, and soil environments, especially where oxidizable sulfur is abundant (e.g., sulfur springs, sulfide minerals, sulfur deposits, sewage treatment areas, and sources of sulfur gases, such as sediments or anaerobic soils releasing H 2 S). The mol % G + C of the DNA is : 62–67. Type species : Thiobacillus thioparus Beijerinck 1904b, 597.

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Acidithiobacillus

Kelly¹,

Wood²

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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A.ci.di.thi.o.ba.cil' lus . L. adj. acidus sour; Gr. n. thios sulfur; L. n. bacillus a small rod; M.L. masc. n. Thiobacillus small acid‐loving sulfur rod. Proteobacteria / Gammaproteobacteria / Acidithiobacillales / Acidithiobacillaceae / Acidithiobacillus Obligately acidophilic, aerobic, Gram‐negative rods, 0.4 × 2.0 µm, motile by one or more flagella. Optimum growth below pH 4.0, using reduced sulfur compounds to support autotrophic growth. Some species oxidize ferrous iron or use natural and synthetic metal sulfides for energy generation; some species oxidize hydrogen. Optimum temperature 30–35°C for mesophilic species and 45°C for moderately thermophilic species. Contain ubiquinone Q‐8. Member of the Gammaproteobacteria . The mol % G + C of the DNA is : 52–64. Type species : Acidithiobacillus thiooxidans (Waksman and Joffe 1922) Kelly and Wood 2000, 513 ( Thiobacillus thiooxidans Waksman and Joffe 1922, 239.)

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Genomic and physiological diversity amongst strains of Thiobacillus ferrooxidans, and genomic comparison with Thiobacillus thiooxidans

Cited by 125 publications

References 34 publications

Phylogenetic heterogeneity of the species Acidithiobacillus ferrooxidans

Phylogenetic heterogeneity of the species Acidithiobacillus ferrooxidans

Thiobacillus

Acidithiobacillus

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