Genomic and transcriptomic characterization of the transcription factor family R2R3-MYB in soybean and its involvement in the resistance responses to Phakopsora pachyrhizi

Aoyagi, Luciano Nobuhiro; Lopes-Caitar, Valéria Stefania; Carvalho, M. C. C. G. de; Darben, Luana Mieko; Polizel-Podanosqui, A. M.; Kuwahara, Marcia Kamogae; Nepomuceno, Alexandre Lima; Abdelnoor, R. V.; Marcelino-Guimarães, Francismar Corrêa

doi:10.1016/j.plantsci.2014.08.005

Cited by 30 publications

(25 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The protein conserved domain prediction was performed using SMART 10 and CD-search service 11 . Motif analysis was performed by MEME 12 with the following parameters: repetitions per sequence = zero or one per sequence; maximum number of motifs found = 12; and an ideal motif size between 6 and 300 amino acids ( Aoyagi et al, 2014 ). Jpred 13 was employed to predict the secondary structure.…”

Section: Methodsmentioning

confidence: 99%

Identification and Phylogenetic Analysis of a Novel Starch Synthase in Maize

et al. 2015

View full text Add to dashboard Cite

Starch is an important reserve of carbon and energy in plants, providing the majority of calories in the human diet and animal feed. Its synthesis is orchestrated by several key enzymes, and the amount and structure of starch, affecting crop yield and quality, are determined mainly by starch synthase (SS) activity. To date, five SS isoforms, including SSI-IV and Granule Bound Starch Synthase (GBSS) have been identified and their physiological functions have been well characterized. Here, we report the identification of a new SS isoform in maize, designated SSV. By searching sequenced genomes, SSV has been found in all green plants with conserved sequences and gene structures. Our phylogenetic analysis based on 780 base pairs has suggested that SSIV and SSV resulted from a gene duplication event, which may have occurred before the algae formation. An expression profile analysis of SSV in maize has indicated that ZmSSV is mainly transcribed in the kernel and ear leaf during the grain filling stage, which is partly similar to other SS isoforms. Therefore, it is likely that SSV may play an important role in starch biosynthesis. Subsequent analysis of SSV function may facilitate understanding the mechanism of starch granules formation, number and structure.

show abstract

Section: Methodsmentioning

confidence: 99%

Identification and Phylogenetic Analysis of a Novel Starch Synthase in Maize

et al. 2015

View full text Add to dashboard Cite

show abstract

“…MYB transcription factors have also been shown to play a role in defense against the soybean rust pathogen Phakopsora pachyrhizi Syd. and P. Syd (Aoyagi et al, 2014). Two putative heat shock transcription factors were identified between the markers for the QTL on chromosome 14 conferring resistance to Py.…”

Section: Fusarium Graminearummentioning

confidence: 99%

High‐Density Mapping of Resistance QTL Toward Phytophthora sojae, Pythium irregulare, and Fusarium graminearum in the Same Soybean Population

et al. 2016

View full text Add to dashboard Cite

Phytophthora sojae Kaufm. and Gerd., Pythium irregulare Busiman, and Fusarium graminearum Schwabe [teleomorph: Gibberella zeae (Schwien.) Petch] are important pathogens of soybean [Glycine max (L.) Merr.] and are all capable of causing seed rot, damping‐off, and root rot. The objective of this study was to identify quantitative trait loci (QTL) for resistance to Py. irregulare and to refine previously mapped QTL for resistance to P. sojae and F. graminearum in a larger, more advanced ‘Conrad’ × ‘Sloan’ F9:11 recombinant inbred line population. The population was mapped with 1032 single nucleotide polymorphisms from the SoySNP6K BeadChip and 31 polymerase chain reaction–based molecular markers. Families were evaluated for resistance response to three isolates of P. sojae, one isolate of Py. irregulare, and one isolate of F. graminearum. A total of 10, 2, and 3 QTL and suggestive QTL were found that confer resistance to P. sojae, Py. irregulare, and F. graminearum, respectively. Individual QTL explained 2 to 13.6% of the phenotypic variance. Quantitative trait loci for resistance toward both Py. irregulare and F. graminearum colocalized on chromosome 19. This resistance was contributed by Sloan and was juxtaposed to a QTL for P. sojae with resistance contributed from Conrad. Alleles for resistance to different pathogens contributed from different parents in the same region; the number of unique QTL for each pathogen and the lack of correlation of resistance suggest that different mechanisms are involved in resistance toward P. sojae, Py. irregulare, and F. graminearum.

show abstract

“…The phenylpropanoids are plant secondary metabolites derived from aromatic amino acids. The induction of phenylpropanoid, flavonoid, and isoflavonoid metabolic pathway genes as a defense response to P. pachyrhizi was reported in transcriptome studies of susceptible and resistant soybean genotypes [46][47][48] . We putatively identified 16 phenylpropanoids ( Supplementary Table S1).…”

Section: Discussionmentioning

confidence: 85%

Unraveling Asian Soybean Rust metabolomics using mass spectrometry and Molecular Networking approach

Silva

Graça

Porto

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Asian Soybean Rust (ASR), caused by the biotrophic fungus Phakopsora pachyrhizi, is a devastating disease with an estimated crop yield loss of up to 90%. Yet, there is a nerf of information on the metabolic response of soybean plants to the pathogen Untargeted metabolomics and Global natural products Social Molecular networking platform approach was used to explore soybean metabolome modulation to P. pachyrhizi infection. Soybean plants susceptible to ASR was inoculated with P. pachyrhizi spore suspension and non-inoculated plants were used as controls. Leaves from both groups were collected 14 days post-inoculation and extracted using different extractor solvent mixtures. The extracts were analyzed on an ultra-high performance liquid chromatography system coupled to high-definition electrospray ionization-mass spectrometry. There was a significant production of defense secondary metabolites (phenylpropanoids, terpenoids and flavonoids) when P. pachyrhizi infected soybean plants, such as putatively identified liquiritigenin, coumestrol, formononetin, pisatin, medicarpin, biochanin A, glyoceollidin i, glyoceollidin ii, glyoceollin i, glyoceolidin ii, glyoceolidin III, glyoceolidin IV, glyoceolidin VI. Primary metabolites (amino acids, peptides and lipids) also were putatively identified. This is the first report using untargeted metabolomics and GNPS-Molecular Networking approach to explore ASR in soybean plants. Our data provide insights into the potential role of some metabolites in the plant resistance to ASR, which could result in the development of resistant genotypes of soybean to P. pachyrhizi, and effective and specific products against the pathogen. The soybean (Glycine max (L.) Merrill) is prominent among crops due to its agro-economic and nutritional value, used mainly as a source of proteins and oils for human consumption, in animal feeds and for biofuel production 1,2. However, estimates indicate a necessity to double global agricultural output by 2050 to feed a rising population, hence soybean productivity needs to be increased by 2.4% per annum 3. Yet, soybean diseases were estimated to reduce crop yield by 11% in the United States 4 and 50% or greater in the southeastern United States 5. The Asian Soybean Rust (ASR) was registered for the first time in Brazil during the 2001/2002 harvest 6. Recently, Brazilian soybean farmers spent US$ 2.16 billion with fungicides during the 2016/2017 harvest, and 96% of the sum was used for ASR control. Another US$ 1.62 billion was invested on insecticides, totaling US$ 3.78 billion. This amount represented 12.4% of the production costs for the harvest 7. Despite increasing costs, disease management practices are needed as yield losses of up to 90% have already been reported when control measures were absent 4 .

show abstract

Genomic and transcriptomic characterization of the transcription factor family R2R3-MYB in soybean and its involvement in the resistance responses to Phakopsora pachyrhizi

Cited by 30 publications

References 39 publications

Identification and Phylogenetic Analysis of a Novel Starch Synthase in Maize

Identification and Phylogenetic Analysis of a Novel Starch Synthase in Maize

High‐Density Mapping of Resistance QTL Toward Phytophthora sojae, Pythium irregulare, and Fusarium graminearum in the Same Soybean Population

Unraveling Asian Soybean Rust metabolomics using mass spectrometry and Molecular Networking approach

Contact Info

Product

Resources

About