Genomic characteristics of Listeria monocytogenes isolated during mushroom (Agaricus bisporus) production and processing

Lake, Frank B.; Overbeek, L.S. van; Baars, J.J.P.; Koomen, Jeroen; Abee, Tjakko; Besten, H.M.W. den

doi:10.1016/j.ijfoodmicro.2021.109438

Cited by 19 publications

(12 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…L. monocytogenes carry at least two genetic determinants that enhance QA tolerance, such as qacH and bcrABC , which codify for efflux pump proteins [ 83 , 84 ]. All the isolates CC5 (ST5) carrying the complete bcrABC operon showed the highest QA tolerance, similar to reports for an isolated obtained from a mushroom production factory in Netherland [ 85 ]. It has been reported that isolates carrying these genes can tolerate 5–13 ppm QA in vitro [ 86 ].…”

Section: Discussionsupporting

confidence: 81%

Evaluation of the Persistence and Characterization of Listeria monocytogenes in Foodservice Operations

Toro

Williams-Vergara

Solar

et al. 2022

Foods

View full text Add to dashboard Cite

Listeria monocytogenes is a major foodborne pathogen that can contaminate food products and colonize food-producing facilities. Foodservice operations (FSOp) are frequently responsible for foodborne outbreaks due to food safety practices failures. We investigated the presence of and characterized L. monocytogenes from two FSOp (cafeterias) distributing ready-to-eat meals and verified FSOp’s compliance with good manufacturing practices (GMP). Two facilities (FSOp-A and FSOp-B) were visited three times each over 5 months. We sampled foods, ingredients, and surfaces for microbiological analysis, and L. monocytogenes isolates were characterized by phylogenetic analyses and phenotypic characteristics. GMP audits were performed in the first and third visits. A ready-to-eat salad (FSOp-A) and a frozen ingredient (FSOp-B) were contaminated with L. monocytogenes, which was also detected on Zone 3 surfaces (floor, drains, and a boot cover). The phylogenetic analysis demonstrated that FSOp-B had persistent L. monocytogenes strains, but environmental isolates were not closely related to food or ingredient isolates. GMP audits showed that both operations worked under “fair” conditions, and “facilities and equipment” was the section with the least compliances. The presence of L. monocytogenes in the environment and GMP failures could promote food contamination with this pathogen, presenting a risk to consumers.

show abstract

Section: Discussionsupporting

confidence: 81%

Evaluation of the Persistence and Characterization of Listeria monocytogenes in Foodservice Operations

Toro

Williams-Vergara

Solar

et al. 2022

Foods

View full text Add to dashboard Cite

show abstract

“…The virulence genes analyzed support the pathogenicity of 11 out of the 14 L. monocytogenes isolates found in this study. The three exceptions were due to the truncation of the actA , as recently described [ 51 ]. The actA gene plays a role in intracellular motility and intercellular spreading, a key determinant of L. monocytogenes virulence [ 52 ].…”

Section: Discussionmentioning

confidence: 99%

Distribution and Characteristics of Listeria spp. in Pigs and Pork Production Chains in Germany

et al. 2022

View full text Add to dashboard Cite

Listeria (L.) monocytogenes is a foodborne pathogen that can cause disease, mainly in elderly, pregnant or immunocompromised persons through consumption of contaminated food, including pork products. It is widespread in the environment and can also be found in asymptomatic carrier animals, for example, in different tissues of pigs. To learn more about their nature, 16 Listeria spp. isolates found in tonsils and intestinal content of pigs and 13 isolates from the slaughterhouse environment were characterized using next-generation sequencing (NGS). A wide distribution of clonal complexes was observed in pigs, as well as in the pork production chain, suggesting multiple sources of entry. Hypervirulent clones were found in pig tonsils, showing the potential risk of pigs as source of isolates causing human disease. The presence of closely related isolates along the production chain suggests a cross-contamination in the slaughterhouse or recontamination from the same source, strengthening the importance of efficient cleaning and disinfection procedures. The phenotypical antimicrobial resistance status of L. monocytogenes isolates was examined via broth microdilution and revealed a low resistance level. Nevertheless, genotypical resistance data suggested multiple resistances in some non-pathogenic L. innocua isolates from pig samples, which might pose a risk of spreading resistances to pathogenic species.

show abstract

“…Here, we showed that the Lm CC388 strain presents more interactions with the host cells, causes more hemolysis and is more pathogenic in an animal model than the Lm CC1 and ATCC 19115 strains, suggesting that the virulence of the Lm CC388 strain has contributed to the development of listeriosis in Spain. Evidence supports this hypothesis because different studies have reported that strains belonging to CC1 are major contributors to human listeriosis, and are considered hypervirulent strains as well [ 4 , 19 , 21 , 22 , 23 ] as they cause further invasion and colonization of tissue, such as the intestinal lumen [ 17 ]. Infections caused by these strains are linked to an unfavorable evolution of listeriosis, triggering rhombencephalitis and miscarriage [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

In Vitro and In Vivo Virulence Study of Listeria monocytogenes Isolated from the Andalusian Outbreak in 2019

et al. 2023

View full text Add to dashboard Cite

In 2019, the biggest listeriosis outbreak by Listeria monocytogenes (Lm) in the South of Spain was reported, resulting in the death of three patients from 207 confirmed cases. One strain, belonging to clonal complex 388 (Lm CC388), has been isolated. We aimed to determine the Lm CC388 virulence in comparison with other highly virulent clones such as Lm CC1 and Lm CC4, in vitro and in vivo. Four L. monocytogenes strains (Lm CC388, Lm CC1, Lm CC4 and ATCC 19115) were used. Attachment to human lung epithelial cells (A549 cells) by these strains was characterized by adherence and invasion assays. Their cytotoxicities to A549 cells were evaluated by determining the cells viability. Their hemolysis activity was determined also. A murine intravenous infection model using these was performed to determine the concentration of bacteria in tissues and blood. Lm CC388 interaction with A549 cells is non-significantly higher than that of ATCC 19115 and Lm CC1, and lower than that of Lm CC4. Lm CC388 cytotoxicity is higher than that of ATCC 19115 and Lm CC1, and lower than that of Lm CC4. Moreover, Lm CC388 hemolysis activity is lower than that of the Lm CC4 strain, and higher than that of Lm CC1. Finally, in the murine intravenous infection model by Lm CC388, higher bacterial loads in tissues and at similar levels of Lm CC4 were observed. Although a lower rate of mortality of patients during the listeriosis outbreak in Spain in 2019 has been reported, the Lm CC388 strain has shown a greater or similar pathogenicity level in vitro and in an animal model, like Lm CC1 and Lm CC4.

show abstract

Genomic characteristics of Listeria monocytogenes isolated during mushroom (Agaricus bisporus) production and processing

Cited by 19 publications

References 46 publications

Evaluation of the Persistence and Characterization of Listeria monocytogenes in Foodservice Operations

Evaluation of the Persistence and Characterization of Listeria monocytogenes in Foodservice Operations

Distribution and Characteristics of Listeria spp. in Pigs and Pork Production Chains in Germany

In Vitro and In Vivo Virulence Study of Listeria monocytogenes Isolated from the Andalusian Outbreak in 2019

Contact Info

Product

Resources

About