Genomic characteristics of listeria that caused invasive listeriosis during the COVID-19 pandemic

Voronina, Olga; Kunda, Marina S.; Рыжова, Н. Н.; Aksenova, Ekaterina I.; Kutuzova, A.V.; Tikulmina, Anna N.; Karpova, Tatiana I.; Melkumyan, A.R.; Климова, Е А; Груздева, О. А.; Tartakovsky, Igor

doi:10.21203/rs.3.rs-1909768/v1

Cited by 3 publications

(8 citation statements)

References 89 publications

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“…Three clinical and two isolates from the restaurant cooking environment had two loci with SNV. An increase in the sample of the ST37 isolates confirmed the previously identified absence of the vip gene in Listeria of this genotype [18].…”

Section: Virulence Factorssupporting

confidence: 83%

“…MultiLocus Sequence Typing (MLST) and Multi-Virulent-Locus Sequence Typing (MvLST) were performed as described earlier [18]. The MvLST scheme included four internalin genes (inlABCE).…”

Section: 22mentioning

confidence: 99%

“…Now patients who have had COVID-19 have become sensitive to L. monocytogenes ST8, ST21, ST37, ST391, and ST425. The range of products from which L. monocytogenes ST37 was isolated has been expanded [18].…”

Section: Introductionmentioning

confidence: 99%

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Listeria monocytogenes ST37 Distribution in Russia and Properties of the Clinical and Food-Born Isolates

Voronina,

Kunda,

Ryzhova

et al. 2023

Preprint

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Listerias of the phylogenetic lineage II (PLII) are common in the European environment, and are hypovirulent. Despite this, they caused more than a third of sporadic cases of listeriosis, and multi-country foodborne outbreaks. L. monocytogenes ST37 is one of them. During the COVID-19 pandemic ST37 appeared in the clinical cases and ranked second in occurrence among food isolates in the Moscow region. The aim of this study was to describe the genomic features of ST37 isolates from different sources. All clinical cases of ST37 were in the cohort of male patients (age, 48-81 years) with meningitis-septicemia manifestation, and COVID-19 or Influenza in the anamnesis. The core genomes of the fish isolates were closely related. The clinical and meat isolates revealed a large diversity. Prophages (2-4/genome) were the source of the unique genes. Two clinical isolates displayed the pseudolysogeny, and excided prophages were A006-like. Absence of the plasmids, the assortment of virulence factors and resistance determinants in chromosome corresponded to the hypovirulent characteristics. However, all clinical isolates caused severe disease with deaths in four cases. Thus, these studies allow us to speculate that a previous viral infection increases a human susceptibility to listeriosis.

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Section: Virulence Factorssupporting

confidence: 83%

“…MultiLocus Sequence Typing (MLST) and Multi-Virulent-Locus Sequence Typing (MvLST) were performed as described earlier [18]. The MvLST scheme included four internalin genes (inlABCE).…”

Section: 22mentioning

confidence: 99%

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Listeria monocytogenes ST37 Distribution in Russia and Properties of the Clinical and Food-Born Isolates

Voronina,

Kunda,

Ryzhova

et al. 2023

Preprint

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show abstract

“…The crucial change in the spectrum of the L. monocytogenes ST causing invasive listeriosis was indicated during the COVID-19 pandemic [18]. Now patients who have had COVID-19 have become sensitive to L. monocytogenes ST8, ST21, ST37, ST391, and ST425.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Listeria monocytogenes ST37 Distribution in the Moscow Region and Properties of Clinical and Foodborne Isolates

Voronina,

Kunda,

Ryzhova

et al. 2023

Life

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Listerias of the phylogenetic lineage II (PLII) are common in the European environment and are hypovirulent. Despite this, they caused more than a third of the sporadic cases of listeriosis and multi-country foodborne outbreaks. L. monocytogenes ST37 is one of them. During the COVID-19 pandemic, ST37 appeared in clinical cases and ranked second in occurrence among food isolates in the Moscow region. The aim of this study was to describe the genomic features of ST37 isolates from different sources. All clinical cases of ST37 were in the cohort of male patients (age, 48–81 years) with meningitis–septicemia manifestation and COVID-19 or Influenza in the anamnesis. The core genomes of the fish isolates were closely related. The clinical and meat isolates revealed a large diversity. Prophages (2–4/genome) were the source of the unique genes. Two clinical isolates displayed pseudolysogeny, and excided prophages were A006-like. In the absence of plasmids, the assortment of virulence factors and resistance determinants in the chromosome corresponded to the hypovirulent characteristics. However, all clinical isolates caused severe disease, with deaths in four cases. Thus, these studies allow us to speculate that a previous viral infection increases human susceptibility to listeriosis.

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Distribution of antimicrobial resistance among clinical and food Listeria monocytogenes isolated in Moscow in 2019–2021

Ermolaeva

Карпова

Andriyanov³

et al. 2022

CMAC

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Objective. To determine the current state-of-art of acquired resistance to antimicrobial drugs among L. monocytogenes strains associated with listeriosis in humans and food contamination in Moscow. Materials and Methods. We used 39 L. monocytogenes strains isolated in Moscow in 2019–2021 from clinical material (n = 24) and food (n = 15). Resistance to 12 antibiotics of the first and second lines of defense was studied using disk-diffusion method. The parameters recommended for L. monocytogenes were used to interpret the results; in the absence of recommendations for L. monocytogenes, the criteria for Staphylococcus aureus and/or Enterococcus spp. were used. Results. All strains were susceptible to ampicillin, benzylpenicillin, erythromycin, vancomycin, imipenem, linezolid, and the amoxicillin/clavulanic acid. Resistance was observed to gentamicin (23%) as well as to meropenem, trimethoprim/sulfamethoxazole and ciprofloxacin (5%, 74% and 28% of strains, respectively). A total of 15 strains with multiple antibiotic resistance were identified (13 and 2 isolates were resistant to three and four antibiotics, respectively). Simultaneous resistance to trimethoprim/sulfamethoxazole, ciprofloxacin and levofloxacin was observed in 9 strains, 6 strains were resistant to gentamicin and trimethoprim/sulfamethoxazole, including 3 strains – to gentamicin, trimethoprim/sulfamethoxazole, levofloxacin, and 2 strains – to gentamicin, trimethoprim/sulfamethoxazole, ciprofloxacin. Comparison of the growth inhibition zones by ampicillin and benzylpenicillin in the studied strains with historical data on the strains isolated in Russia in 1950–1980 showed a significant downward shift in the size of growth inhibition zones. Comparison of the distribution of strains with different diameters of growth inhibition zones depending on the source of isolation did not show significant differences between clinical strains and strains of food origin isolated in 2019–2021. Conclusions. A wide spread of acquired resistance was shown among L. monocytogenes strains of clinical and food origin isolated in Moscow in 2019–2021. Despite the fact that all strains were susceptible to penicillins, the distribution of growth inhibition zone diameters showed a significant shift towards decreasing sensitivity to ampicillin and benzylpenicillin in strains isolated in 2019–2021 compared with L. monocytogenes strains isolated in Russia before 1980.

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Genomic characteristics of listeria that caused invasive listeriosis during the COVID-19 pandemic

Cited by 3 publications

References 89 publications

Listeria monocytogenes ST37 Distribution in Russia and Properties of the Clinical and Food-Born Isolates

Listeria monocytogenes ST37 Distribution in Russia and Properties of the Clinical and Food-Born Isolates

Listeria monocytogenes ST37 Distribution in the Moscow Region and Properties of Clinical and Foodborne Isolates

Distribution of antimicrobial resistance among clinical and food Listeria monocytogenes isolated in Moscow in 2019–2021

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