Genomic characterization and seed transmission of a novel unclassified partitivirus infecting Polygonatum kingianum Coll. et Hemsl

Zhang, Fan; Su, Xiaoxia; Zhang, Shaozhi; Wang, Maosen; Wang, Tiantian; Xue, Zheng; Wu, Kai; Zheng, Kuanyu; Zhang, Zhongkai

doi:10.1016/j.heliyon.2023.e16719

Cited by 4 publications

(4 citation statements)

References 21 publications

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“…In plants, partitivirus infections are transmitted vertically through seeds [ 19 ], and vertical transmission may occur in wild plants or crops. Seed infection provides the virus with suitable conditions to persist for long periods when the hosts and/or vectors are not available, because viral agents can survive within the seeds as long as it remains viable [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

“…Viruses can reach the seeds by direct invasion of embryonic tissue and by infection of the ovules or pollen [ 21 ]. In several plant species, viral infection of the seeds has been proven, for example in Polygonatum kingianum , a high infection rate was found in the seeds by the Polygonatum kingianum cryptic virus 1 [ 19 ]. Other examples include the Raphanus sativus partitivirus 1 that infects Raphanus sativus ; the Sinapis alba cryptic virus 1 that infects Sinapis alba ; and the Brassica rapa cryptic virus 1 that infects Brassica rapa [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

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A Novel Cryptic Virus Isolated from Galphimia spp. in Mexico

Iglesias,

Stevens,

Sharma

et al. 2024

Pathogens

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Galphimia spp. is a plant employed in traditional medicine in Mexico because of its anxiolytic and sedative effects. Viruses have been associated with different alterations in plants, although asymptomatic agents (i.e., cryptic viruses) are also known. High-throughput sequencing (HTS) allows for the detection of pathogenic and non-pathogenic viral agents in plants, including potential novel viruses. The aim of this study was to investigate the presence of viral agents in two populations of Galphimia spp. by HTS. Sequencing was conducted on an Illumina NextSeq 550 platform, and a putative novel virus was identified. Two contigs showed homology to partitiviruses, and these encoded the RNA-dependent RNA polymerase and coat protein. These proteins showed the highest identities with orthologs in the recently discovered Vitis cryptic virus. A phylogenetic analysis of both RNAs showed that the new virus clusters into the monophyletic genus Deltapartitivirus along with other plant-infecting viruses. The result of the HTS analysis was validated by conventional RT-PCR and Sanger sequencing. A novel virus was discovered in a symptomless Galphimia spp. plant and tentatively named the Galphimia cryptic virus (GCV). This is the first virus discovered in medicinal plants in Mexico.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A Novel Cryptic Virus Isolated from Galphimia spp. in Mexico

Iglesias,

Stevens,

Sharma

et al. 2024

Pathogens

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“…Viruses are the important pathogens that cause considerable yield and quality loss in P. kingianum. Currently, there is a total of six viruses reported infecting P. kingianum, including apple stem grooving virus (ASGV) [4], bean common mosaic virus (BCMV) [5], polygonatum kingianum virus 1(PKgV1) [6], paris mosaic necrosis virus (PMNV) [7], polygonatum mosaic-associated virus 1 (PMaV1) [8], and polygonatum kingianum cryptic virus 1 (PKCV1) [9]. In this study, the complete genome sequence of a putative novel potyvirus infecting P. kingianum was obtained and subjected to molecular characterization.…”

Section: Full Textmentioning

confidence: 99%

“…PKYLV has 10,002 nt in total, which form a large open reading frame (ORF) encoding a 3,213 amino acid (aa) polyprotein with a molecular weight of about 363.68 kDa. The ORF starts with codon AUG (108-110) and ends with codon UGA (9,746). The polyprotein comprises 10 mature proteins, including P1 (432aa), HC-Pro (459aa), P3 (352aa), 6K1 (52aa), CI (643aa), 6K2 (53aa), NIa-Pro (243aa), NIb (517aa), and CP (267aa) (Fig.…”

Section: Full Textmentioning

confidence: 99%

Complete genome sequence of polygonatum kingianum mottle virus infecting Polygonatum kingianum Coll. et Hemsl in Yunnan, China

Wang,

Su,

Zhang

et al. 2024

Arch Virol

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The complete genome sequence of a putative novel potyvirus, tentatively named polygonatum kingianum yellow leafroll virus (PKYLV; GenBank accession no. ON428226), infecting Polygonatum. kingianum in China was obtained by next-generation sequencing (NGS), reverse transcription-polymerase chain reaction (RT-PCR) and rapid ampli cation of cDNA ends (RACE). PKYLV exhibits typical genomic structure characteristics of genus Potyvirus, with a length of 10,002 nucleotides (nt) and a large open reading frame (108 to 9,746 nt), encoding a polyprotein with 3,213 amino acid (aa) (363.68 kDa).Pairwise comparisons revealed that the PKYLV polyprotein shares 50.5 68.6% nt and 43.1 72.2% aa sequence identity with reported members of the genus Potyvirus. Moreover, phylogenetic analysis indicated that PKYLV is closely related to polygonatum kingianum virus 1 (PKgV1; accession no. MK427056). These results suggest that the PKYLV is a novel member of the genus Potyvirus of the family Potyviridae.

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Molecular characterization of the complete genome sequence of polygonatum kingianum yellow leafroll virus (PKYLV) infecting Polygonatum kingianum Coll. et Hemsl in Yunnan, China

Zheng,

Zhang

2023

Preprint

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The complete genome sequence of a putative novel potyvirus, tentatively named polygonatum kingianum yellow leafroll virus (PKYLV; GenBank accession no. ON428226), infecting Polygonatum. kingianum in China was obtained by next-generation sequencing (NGS), reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). PKYLV exhibits typical genomic structure characteristics of genus Potyvirus, with a length of 10,002 nucleotides (nt) and a large open reading frame (108 to 9,746 nt), encoding a polyprotein with 3,213 amino acid (aa) (363.68 kDa). Pairwise comparisons revealed that the PKYLV polyprotein shares 50.5～68.6% nt and 43.1～72.2% aa sequence identity with reported members of the genus Potyvirus. Moreover, phylogenetic analysis indicated that PKYLV is closely related to polygonatum kingianum virus 1 (PKgV1; accession no. MK427056). These results suggest that the PKYLV is a novel member of the genus Potyvirus of the family Potyviridae.

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Genomic characterization and seed transmission of a novel unclassified partitivirus infecting Polygonatum kingianum Coll. et Hemsl

Cited by 4 publications

References 21 publications

A Novel Cryptic Virus Isolated from Galphimia spp. in Mexico

A Novel Cryptic Virus Isolated from Galphimia spp. in Mexico

Complete genome sequence of polygonatum kingianum mottle virus infecting Polygonatum kingianum Coll. et Hemsl in Yunnan, China

Molecular characterization of the complete genome sequence of polygonatum kingianum yellow leafroll virus (PKYLV) infecting Polygonatum kingianum Coll. et Hemsl in Yunnan, China

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