Genomic Characterization of a Novel Gut Symbiont From the Hadal Snailfish

Lian, Chun-Ang; Yan, Guoyong; Huang, Junzhe; Danchin, Antoine; Wang, Yong; He, Lisheng

doi:10.3389/fmicb.2019.02978

Cited by 38 publications

(29 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a member of this bacterial family, Mycoplasma have been previously identified in the gut of other fish such as Atlantic salmon, Chinook salmon (Oncorhynchus tshawytscha), zebrafish (Danio rerio), common carp (Cyprinus carpio), largemouth bronze gudgeon (Coreius guichenoti) and rainbow trout [38][39][40][41][42][43][44]. While recent genome reconstruction indicates a mutualistic lifestyle of this organism in the intestine of certain species like Atlantic salmon and hadal snailfish (Pseudoliparis swirei) [45,46], for others it has been associated with disease [42]. Members of the genus Allivibrio largely form part of the natural gut microbiota of various fish (e.g.…”

Section: Discussionmentioning

confidence: 99%

Antibiotic-induced alterations and repopulation dynamics of yellowtail kingfish microbiota

et al. 2020

View full text Add to dashboard Cite

Background: The use of antibiotics in aquaculture is a common infection treatment and is increasing in some sectors and jurisdictions. While antibiotic treatment can negatively shift gut bacterial communities, recovery and examination of these communities in fish of commercial importance is not well documented. Examining the impacts of antibiotics on farmed fish microbiota is fundamental for improving our understanding and management of healthy farmed fish. This work assessed yellowtail kingfish (Seriola lalandi) skin and gut bacterial communities after an oral antibiotic combination therapy in poor performing fish that displayed signs of enteritis over an 18-day period. In an attempt to promote improved bacterial re-establishment after antibiotic treatment, faecal microbiota transplantation (FMT) was also administered via gavage or in the surrounding seawater, and its affect was evaluated over 15 days post-delivery. Results: Antibiotic treatment greatly perturbed the global gut bacterial communities of poor-performing fishan effect that lasted for up to 18 days post treatment. This perturbation was marked by a significant decrease in species diversity and evenness, as well as a concomitant increase in particular taxa like an uncultured Mycoplasmataceae sp., which persisted and dominated antibiotic-treated fish for the entire 18-day period. The skinassociated bacterial communities were also perturbed by the antibiotic treatment, notably within the first 3 days; however, this was unlike the gut, as skin microbiota appeared to shift towards a more 'normal' (though disparate) state after 5 days post antibiotic treatment. FMT was only able to modulate the impacts of antibiotics in some individuals for a short time period, as the magnitude of change varied substantially between individuals. Some fish maintained certain transplanted gut taxa (i.e. present in the FMT inoculum; namely various Aliivibrio related ASVs) at Day 2 post FMT, although these were lost by Day 8 post FMT. Conclusion: As we observed notable, prolonged perturbations induced by antibiotics on the gut bacterial assemblages, further work is required to better understand the processes/dynamics of their re-establishment following antibiotic exposure. In this regard, procedures like FMT represent a novel approach for promoting improved microbial recovery, although their efficacy and the factors that support their success requires further investigation.

show abstract

Section: Discussionmentioning

confidence: 99%

Antibiotic-induced alterations and repopulation dynamics of yellowtail kingfish microbiota

et al. 2020

View full text Add to dashboard Cite

show abstract

“…In fact, this genus already includes known fish pathogens, such as Mycoplasma mobile , a bacterium colonizing the gills of freshwater fish [ 51 ]. In contrast, recent genome reconstruction analysis revealed a mutualistic lifestyle of new Mycoplasma species isolated from Atlantic salmon and hadal snailfish ( Pseudoliparis swirei ) [ 50 , 52 ]. In our study, it is unclear whether this bacterium played a role in the disease, or was the result of the poor overall health of the fish.…”

Section: Discussionmentioning

confidence: 99%

Investigating Both Mucosal Immunity and Microbiota in Response to Gut Enteritis in Yellowtail Kingfish

et al. 2020

View full text Add to dashboard Cite

The mucosal surfaces of fish play numerous roles including, but not limited to, protection against pathogens, nutrient digestion and absorption, excretion of nitrogenous wastes and osmotic regulation. During infection or disease, these surfaces act as the first line of defense, where the mucosal immune system interacts closely with the associated microbiota to maintain homeostasis. This study evaluated microbial changes across the gut and skin mucosal surfaces in yellowtail kingfish displaying signs of gut inflammation, as well as explored the host gene expression in these tissues in order to improve our understanding of the underlying mechanisms that contribute to the emergence of these conditions. For this, we obtained and analyzed 16S rDNA and transcriptomic (RNA-Seq) sequence data from the gut and skin mucosa of fish exhibiting different health states (i.e., healthy fish and fish at the early and late stages of enteritis). Both the gut and skin microbiota were perturbed by the disease. More specifically, the gastrointestinal microbiota of diseased fish was dominated by an uncultured Mycoplasmataceae sp., and fish at the early stage of the disease showed a significant loss of diversity in the skin. Using transcriptomics, we found that only a few genes were significantly differentially expressed in the gut. In contrast, gene expression in the skin differed widely between health states, in particular in the fish at the late stage of the disease. These changes were associated with several metabolic pathways that were differentially expressed and reflected a weakened host. Altogether, this study highlights the sensitivity of the skin mucosal surface in response to gut inflammation.

show abstract

“…As a member of this bacterial family, Mycoplasma have been previously identi ed in the gut of other sh such as Atlantic salmon, Chinook salmon (Oncorhynchus tshawytscha), zebra sh (Danio rerio), common carp (Cyprinus carpio), largemouth bronze gudgeon (Coreius guichenoti) and rainbow trout [38][39][40][41][42][43][44]. While recent genome reconstruction indicates a mutualistic lifestyle of this organism in the intestine of certain species like Atlantic salmon and hadal snail sh (Pseudoliparis swirei) [45,46], for others it has been associated with disease [42]. Members of the genus Allivibrio largely form part of the natural gut microbiota of various sh (e.g.…”

Section: Discussionmentioning

confidence: 99%

Antibiotic-induced alterations and repopulation dynamics of yellowtail kingfish microbiota

Legrand

Catalano

Wos‐Oxley

et al. 2020

Preprint

View full text Add to dashboard Cite

Background The use of antibiotics in aquaculture is a common infection treatment and is increasing in some sectors and jurisdictions. While antibiotic treatment can negatively shift gut bacterial communities, recovery and examination of these communities in fish of commercial importance is not well documented. Examining the impacts of antibiotics on farmed fish microbiota is fundamental for improving our understanding and management of healthy farmed fish. This work assessed yellowtail kingfish ( Seriola lalandi ) skin and gut bacterial communities after an oral antibiotic combination therapy in poor performing fish that displayed signs of enteritis over an 18-day period. In an attempt to promote improved bacterial re-establishment after antibiotic treatment, faecal microbiota transplantation (FMT) was also administered via gavage or in the surrounding seawater, and its affect was evaluated over 15 days post-delivery. Results Antibiotic treatment greatly perturbed the global gut bacterial communities of poor-performing fish – an effect that lasted for up to 18 days post treatment. This perturbation was marked by a significant decrease in species diversity and evenness, as well as a concomitant increase in particular taxa like an uncultured Mycoplasmataceae sp., which persisted and dominated antibiotic-treated fish for the entire 18-day period. The skin-associated bacterial communities were also perturbed by the antibiotic treatment, notably within the first 3 days; however, this was unlike the gut, as skin microbiota appeared to shift towards a more ‘normal’ (though disparate) state after 5 days post antibiotic treatment. FMT was only able to modulate the impacts of antibiotics in some individuals for a short time period, as the magnitude of change varied substantially between individuals. Some fish maintained certain transplanted gut taxa (i.e. present in the FMT inoculum; namely various Aliivibrio related ASVs) at Day 2 post FMT, although these were lost by Day 8 post FMT. Conclusion As we observed notable, prolonged perturbations induced by antibiotics on the gut bacterial assemblages, further work is required to better understand the processes/dynamics of their re-establishment following antibiotic exposure. In this regard, procedures like FMT represent a novel approach for promoting improved microbial recovery, although their efficacy and the factors that support their success requires further investigation.

show abstract

Genomic Characterization of a Novel Gut Symbiont From the Hadal Snailfish

Cited by 38 publications

References 65 publications

Antibiotic-induced alterations and repopulation dynamics of yellowtail kingfish microbiota

Antibiotic-induced alterations and repopulation dynamics of yellowtail kingfish microbiota

Investigating Both Mucosal Immunity and Microbiota in Response to Gut Enteritis in Yellowtail Kingfish

Antibiotic-induced alterations and repopulation dynamics of yellowtail kingfish microbiota

Contact Info

Product

Resources

About