Genomic characterization of four novel Staphylococcus myoviruses

Oduor, J.M.O.; Kiljunen, Saija; Kadija, Ermir; Mureithi, Marianne; Nyachieo, Atunga; Skurnik, Mikael

doi:10.1007/s00705-019-04267-0

Cited by 11 publications

(15 citation statements)

References 15 publications

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“…Our rationale to characterize the Stab phages originally isolated using S. xylosus as enrichment host [31] was based on the facts that Staphylococcus phages generally show wide host spectra, that new S. aureus specific phages are not easy to encounter, and that every phage able to infect clinical S. aureus isolates would be a welcome addition to our collection of potential therapeutic phages.…”

Section: Resultsmentioning

confidence: 99%

“…In our previous study, genome sequence and phylogenetic analyses of phages Stab20, Stab21, Stab22, and Stab23, established that the phages are members of the genus Kayvirus which exclusively contains Staphylococcus phages [31]. Electron microscopy revealed that these phages possess icosahedral heads, long contractile tails with baseplates at the end, and tail fibers extending from the baseplates (Figure 1).…”

Section: Morphologymentioning

confidence: 91%

“…They include isolates originating from the Hospital District of Helsinki and Uusimaa Laboratories (HUSLAB, Vantaa, Finland), Finland, and pig isolates [30]. The Staphylococcus xylosus strain DD-34 that was used as a host strain when isolating the phages [31] is a natural sausage fermenter isolated from dried sausage [32]. The incubations of staphylococci and phage isolations were performed at 37 • C using Lysogeny Broth (LB) [33].…”

Section: Bacterial Strains Phages and Mediamentioning

confidence: 99%

“…The isolation of phages Stab20, Stab21, Stab22, and Stab23 from Albanian sewage and river water samples using S. xylosus strain DD-34 as host bacteria was described in [31]. The accession numbers of the DNA sequences of the genomes are LR215718, LR215719, LR215720 and LR215721, respectively.…”

Section: Phage Isolation and Purificationmentioning

confidence: 99%

“…Previous in silico genome analysis of Stab phages predicted the presence of several structural proteins [31]. SDS-PAGE and mass spectrometry (LC-MS/MS) were used to identify the structural and phage particle-associated proteins of Stab20, Stab21, Stab22 and Stab23.…”

Section: Analysis Of Stab Phage Particle Proteomesmentioning

confidence: 99%

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Bioprospecting Staphylococcus Phages with Therapeutic and Bio-Control Potential

Oduor

Kadija

Nyachieo

et al. 2020

Viruses

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Emergence of antibiotic-resistant bacteria is a serious threat to the public health. This is also true for Staphylococcus aureus and other staphylococci. Staphylococcus phages Stab20, Stab21, Stab22, and Stab23, were isolated in Albania. Based on genomic and phylogenetic analysis, they were classified to genus Kayvirus of the subfamily Twortvirinae. In this work, we describe the in-depth characterization of the phages that electron microscopy confirmed to be myoviruses. These phages showed tolerance to pH range of 5.4 to 9.4, to maximum UV radiation energy of 25 µJ/cm2, to temperatures up to 45 °C, and to ethanol concentrations up to 25%, and complete resistance to chloroform. The adsorption rate constants of the phages ranged between 1.0 × 10−9 mL/min and 4.7 × 10−9 mL/min, and the burst size was from 42 to 130 plaque-forming units. The phages Stab20, 21, 22, and 23, originally isolated using Staphylococcus xylosus as a host, demonstrated varied host ranges among different Staphylococcus strains suggesting that they could be included in cocktail formulations for therapeutic or bio-control purpose. Phage particle proteomes, consisting on average of ca 60–70 gene products, revealed, in addition to straight-forward structural proteins, also the presence of enzymes such DNA polymerase, helicases, recombinases, exonucleases, and RNA ligase polymer. They are likely to be injected into the bacteria along with the genomic DNA to take over the host metabolism as soon as possible after infection.

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Section: Resultsmentioning

confidence: 99%

Section: Morphologymentioning

confidence: 91%