Genomic characterization of Kerstersia gyiorum SWMUKG01, an isolate from a patient with respiratory infection in China

Abstract

Background The Gram-negative bacterium Kerstersia gyiorum , a potential etiological agent of clinical infections, was isolated from several human patients presenting clinical symptoms. Its significance as a possible pathogen has been previously overlooked as no disease has thus far been definitively associated with this bacterium. To better understand how the organism contributes to the infectious disease, we determined the complete genomic sequence of K . gyiorum SWMUKG01, the first clinical isolate from southwest China. Results The genomic data obtained displayed a single circular chromosome of 3, 945, 801 base pairs in length, which contains 3, 441 protein-coding genes, 55 tRNA genes and 9 rRNA genes. Analysis on the full spectrum of protein coding genes for cellular structures, two-component regulatory systems and iron uptake pathways that may be important for the success of the bacterial survival, colonization and establishment in the host conferred new insights into the virulence characteristics of K . gyiorum . Phylogenomic comparisons with Alcaligenaceae species indicated that K . gyiorum SWMUKG01 had a close evolutionary relationships with Alcaligenes aquatilis and Alcaligenes faecalis . Conclusions The comprehensive analysis presented in this work determinates for the first time a complete genome sequence of K . gyiorum , which is expected to provide useful information for subsequent studies on pathogenesis of this species.

Antibiotic Profiles and Draft Genome Sequences of Kerstersia gyiorum, Providencia stuartii, Providencia vermicola, and Alcaligenes faecalis Strains Recovered from Soft Tissue Biopsy Samples in Ghana

“…Here, we report on the draft genome sequences and antibiograms of four Gram-negative bacteria species, namely, Kerstersia gyiorum , Alcaligenes faecalis , Providencia stuartii , and Providencia vermicola , that were recovered from human soft tissue biopsy samples. Kerstersia gyiorum represents opportunistic bacteria ( 2 ) that are frequently resistant to chloramphenicol and ciprofloxacin ( 3 , 4 ). Alcaligenes faecalis has been associated with severe clinical conditions in humans ( 5 , 6 ).…”

“… Alcaligenes faecalis has been associated with severe clinical conditions in humans ( 5 , 6 ). Providencia stuartii infections are difficult to manage ( 3 ) and are often resistant to multiple antibiotics ( 7 – 9 ). The NDM-1 carbapenemase gene has been detected in Providencia vermicola isolates ( 10 , 11 ).…”

Analysis of twelve genomes of the bacterium Kerstersia gyiorum from brown-throated sloths (Bradypus variegatus), the first from a non-human host

“…Despite being associated with various diseases, the pathogenic mechanism of K. gyiorum is unclear. The genomic analysis of human isolates allowed the identification of putative virulence factors and antimicrobial resistance genes in K. gyiorum ( Li et al, 2019 ). Strains from non-human hosts may have a different genetic makeup compared to strains of human origin, potentially exhibiting novel metabolic capacities, increased virulence, or zoonotic potential.…”

Characterization of nontypeable Actinobacillus pleuropneumoniae isolates

“…12 Genes that contribute to O-PS biosynthesis may also contribute to CPS biosynthesis, as both antigens are comprised of repeating polysaccharide units, and that genes involved in polysaccharide biosynthesis, polymerization, and transport may naturally be shared among both processes. 3,[11][12][13]21 However, little is known regarding the contribution of A. pleuropneumoniae O-PS biosynthesis genes to the biosynthesis of CPS. Therefore, further studies would be needed to elucidate whether the O-PS biosynthesis genes of A. pleuropneumoniae contribute to the biosynthesis of CPS.…”

“…The Und-P Gal phosphotransferase of A. pleuropneumoniae is homologous to another well-described Und-P Gal phosphotransferase, WbaP. 19 It is well known that WbaP is required to catalyze the transfer of Gal-1-P from uridine-P to the lipid carrier Und-P to yield Und-P-P-Gal, which primes the biosynthesis of both O-PS and CPS in gram-negative bacteria such as group 1 capsule-producing Escherichia coli , 3 Klebsiella pneumoniae , 3 Kerstersia gyiorum , 11 Providencia spp., 13 Salmonella enterica , 3 and Vibrio vulnificus . 12 Genes that contribute to O-PS biosynthesis may also contribute to CPS biosynthesis, as both antigens are comprised of repeating polysaccharide units, and that genes involved in polysaccharide biosynthesis, polymerization, and transport may naturally be shared among both processes.…”