Genomic Characterization of the First Equine-Like G3P[8] Rotavirus Strain Detected in the United States

Perkins, Charity; Mijatovic-Rustempasic, Slavica; Ward, M. Leanne; Cortese, Margaret M.; Bowen, Michael D.

doi:10.1128/genomea.01341-17

Cited by 38 publications

(35 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Only a few equine-like G3P [8] strains were detected in Germany, Hungary, Japan, Thailand, and the USA. 11,17,[22][23][24][25] In Australia and Spain, the detection rates were moderate: 14.4% to 37.6%. 8,16 In contrast, equine-like G3P [8] strains were predominant in Brazil (71.4% in 2016 and 66.2% in 2017) 15,19 and Indonesia (85.7% in 2015-2016).…”

Section: Discussionmentioning

confidence: 99%

“…Although equine‐like G3P[8] strains have been found worldwide, the detection rates are quite different depending on the countries. Only a few equine‐like G3P[8] strains were detected in Germany, Hungary, Japan, Thailand, and the USA . In Australia and Spain, the detection rates were moderate: 14.4% to 37.6% .…”

Section: Discussionmentioning

confidence: 99%

“…G3P[8] strains with a short RNA profile are described as DS‐1‐like (equine‐like) G3P[8] strains since their VP7 gene sequences are highly related to those of RVAs detected in horses . Recently, DS‐1‐like G1P[8] strains and equine‐like G3P[8] strains have spread worldwide …”

Section: Introductionmentioning

confidence: 99%

“…11,[13][14][15] Recently, DS-1-like G1P [8] strains and equine-like G3P [8] strains have spread worldwide. [8][9][10][11][12][14][15][16][17][18][19][20][21][22][23][24][25] In this study, we determined the G/P type distribution and carried out the full-genome characterization of two representative unusual equine-like G3P [8] strains which were highly prevalent (33.7%) in Thailand between January 2014 and September 2016. It is noteworthy that the equine-like G3P [8] strains with a short RNA profile that had been recently detected in several countries were predominant (65.3% to 89.8%) in various age groups across Thailand since 2015.…”

mentioning

confidence: 99%

See 3 more Smart Citations

High prevalence of equine‐like G3P[8] rotavirus in children and adults with acute gastroenteritis in Thailand

Tacharoenmuang

Komoto

Guntapong

et al. 2019

Journal of Medical Virology

View full text Add to dashboard Cite

Group A rotavirus (RVA) is a major cause of acute gastroenteritis in infants and young children worldwide. This study aims to clarify the distribution of G/P types and genetic characteristics of RVAs circulating in Thailand. Between January 2014 and September 2016, 1867 stool specimens were collected from children and adults with acute gastroenteritis in six provinces in Thailand. RVAs were detected in 514/1867 (27.5%) stool specimens. G1P[8] (44.7%) was the most predominant genotype, followed by G3P[8] (33.7%), G2P[4] (11.5%), G8P[8] (7.0%), and G9P[8] (1.3%). Unusual G3P[9] (0.8%), G3P[10] (0.4%), G4P[6] (0.4%), and G10P[14] (0.2%) were also detected at low frequencies. The predominant genotype, G1P[8] (64.4%), in 2014 decreased to 6.1% in 2016. In contrast, the frequency of G3P[8] markedly increased from 5.5% in 2014 to 65.3% in 2015 and 89.8% in 2016. On polyacrylamide gel electrophoresis, most (135/140; 96.4%) of the G3P[8] strains exhibited a short RNA profile. Successful determination of the nucleotide sequences of the VP7 genes of 98 G3P[8] strains with a short RNA profile showed that they are all equine‐like G3P[8] strains. On phylogenetic analysis of genome segments of two representative Thai equine‐like G3P[8] strains, it was noteworthy that they possessed distinct NSP4 genes, one bovine‐like and the other human‐like. Thus, we found that characteristic equine‐like G3P[8] strains with a short RNA electropherotype are becoming highly prevalent in children and adults in Thailand.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

High prevalence of equine‐like G3P[8] rotavirus in children and adults with acute gastroenteritis in Thailand

Tacharoenmuang

Komoto

Guntapong

et al. 2019

Journal of Medical Virology

View full text Add to dashboard Cite

show abstract

“…The first DS-1-like intergenogroup reassortant strains, i.e., DS-1-like G1P [8] strains, were identified in diarrheic children in Japan in 2012 [15,24,33], and subsequently DS-1-like G1P [8] strains were reported from Thailand, Brazil, Malawi, the Philippines, and Vietnam in 2012-2013 [18,21,25,27,33], and Pakistan in 2016 [30]. In 2013, DS-1-like (equine-like) G3P [8] strains emerged in Thailand and Australia [12,22], and equine-like G3P [8] strains have successfully spread to several parts of the world, i.e., Dominica in 2014 [18], Germany, Hungary, Indonesia, Japan, Spain, and the United States in 2015 [6,10,13,20,28,29,32], Brazil in 2016 [16], and Italy in 2018 [14]. In 2013, DS-1-like (bovine-like) G8P [8] strains also emerged in Thailand [31], and subsequently bovine-like G8P [8] strains were detected in the Czech Republic, Japan, and Vietnam in 2014 [17,23,26], and Singapore in 2016 [11].…”

Section: Introductionmentioning

confidence: 99%

Full genome characterization of novel DS-1-like G9P[8] rotavirus strains that have emerged in Thailand

et al. 2020

View full text Add to dashboard Cite

The emergence and rapid spread of unusual DS-1-like intergenogroup reassortant rotaviruses having G1/3/8 genotypes have been recently reported from major parts of the world (Africa, Asia, Australia, Europe, and the Americas). During rotavirus surveillance in Thailand, three novel intergenogroup reassortant strains possessing the G9P[8] genotype (DBM2017-016, DBM2017-203, and DBM2018-291) were identified in three stool specimens from diarrheic children. In the present study, we determined and analyzed the full genomes of these three strains. On full-genomic analysis, all three strains were found to share a unique genotype constellation comprising both genogroup 1 and 2 genes: G9-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2. Phylogenetic analysis demonstrated that each of the 11 genes of the three strains was closely related to that of emerging DS-1-like intergenogroup reassortant, human, and/or locally circulating human strains. Thus, the three strains were suggested to be multiple reassortants that had acquired the G9-VP7 genes from co-circulating Wa-like G9P[8] rotaviruses in the genetic background of DS-1-like intergenogroup reassortant (likely equine-like G3P[8]) strains. To our knowledge, this is the first description of emerging DS-1-like intergenogroup reassortant strains having the G9P[8] genotype. Our observations will add to the growing insights into the dynamic evolution of emerging DS-1like intergenogroup reassortant rotaviruses through reassortment.

show abstract

Emergence of G12P[6] rotavirus strains among hospitalised children with acute gastroenteritis in Belém, Northern Brazil, following introduction of a rotavirus vaccine

et al. 2019

View full text Add to dashboard Cite

Genomic Characterization of the First Equine-Like G3P[8] Rotavirus Strain Detected in the United States

Abstract: We report here the full coding region sequences for all 11 segments of the first equine-like G3P[8] rotavirus strain detected in the United States, strain RVA/Human-wt/USA/3000390639/2015/G3P[8]. The full genotype constellation of this strain is G3-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2.

Cited by 38 publications

References 10 publications

High prevalence of equine‐like G3P[8] rotavirus in children and adults with acute gastroenteritis in Thailand

High prevalence of equine‐like G3P[8] rotavirus in children and adults with acute gastroenteritis in Thailand

Full genome characterization of novel DS-1-like G9P[8] rotavirus strains that have emerged in Thailand

Emergence of G12P[6] rotavirus strains among hospitalised children with acute gastroenteritis in Belém, Northern Brazil, following introduction of a rotavirus vaccine

Contact Info

Product

Resources

About