Genomic comparison of bovine papillomavirus 1 isolates from bovine, equine and asinine lesional tissue samples

Koch, Christoph; Ramsauer, Anna Sophie; Drögemüller, Michaela; Ackermann, Mathias; Gerber, Vinzenz; Tobler, Kurt

doi:10.1016/j.virusres.2017.11.005

Cited by 10 publications

(20 citation statements)

References 27 publications

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“…The IT-1506 genome sequence showed 100% identity with a BPV-1 isolated from a cow fibropapilloma in Switzerland. This result reinforces the concept that the genomic characteristics of BPV-1 are not related to the species from which they are isolated, as stated by Koch and co-workers [ 32 ]. Moreover, the fact that the source of the BPV-1 IT 1506 is a papilloma lesion and not a fibropapilloma led us to speculate that the outcome of the infection might be the result of virus––host interaction.…”

Section: Discussionsupporting

confidence: 89%

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Bovine Papillomavirus 1 Gets Out of the Flock: Detection in an Ovine Wart in Sicily

et al. 2020

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A proliferative cauliflower lesion was excised from the udder of a sheep. Histological investigation confirmed the macroscopic classification of the lesion as a papilloma, without any fibroblastic proliferation. PCR revealed the presence of bovine papillomavirus (BPV), which was further confirmed by the identification of a Deltapapillomavirus 4 by Next Generation Sequencing analysis. This was subsequently classified as bovine papillomavirus type 1. Negative staining electron microscopy (EM) analyses produced negative test results for papillomavirus particles. RNA in situ hybridization (ISH) confirmed the presence of BPV-1. The results further confirm the ability of BPVs belonging to the Deltapapillomavirus genus to infect distantly related species and to cause lesions that are different from sarcoids.

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Section: Discussionsupporting

confidence: 89%

“…Since the farmer reported sharing pasture between cattle and sheep, a contaminated environment could represent the source of the BPV infection. In addition, viral transmission between bovines and equids has also been described as a recurrent and ongoing phenomenon [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Bovine Papillomavirus 1 Gets Out of the Flock: Detection in an Ovine Wart in Sicily

et al. 2020

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“…The sequence analysis of the positive DNA samples revealed the detection of BPV-1 which is the most detected type in different countries [ 2 , 34 ]. It has the ability to infect other species including equine, asinine, and bovine hosts that become a common phenomenon [ 35 ]. According to the general criteria and rules used for PV taxonomy, a new genotype is considered if the comparison with the closest strain revealed variance more than 10% of the L1 gene nucleotide sequence [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular detection and immunopathological examination of Deltapapillomavirus 4 in skin and udder of Egyptian cattle

2018

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Aim:Bovine papillomaviruses (BPVs) are the main cause of bovine papillomatosis resulting in cutaneous and/or mucosal benign tumors that could be transformed to malignant ones with marked economic importance, especially in the dairy farms. Molecular, pathological, and immunohistochemical (IHC) diagnosis of bovine papillomatosis cases was conducted to identify and characterize the circulating BPV genotype in some Egyptian governorates.Materials and Methods:Wart-like lesions in skin, udder, and teats were collected from 123 infected cases in Giza, Beni Suef, and El Menoufia Governorates, Egypt, during 2016-2017. Pathological and IHC characterization, molecular identification, genotyping, and phylogenetic analysis based on the conserved late (L1) gene of the all samples were carried out.Results:89 of the 123 collected samples (72.3%) were positively detected by polymerase chain reaction (PCR). The sequence analysis of the obtained PCR amplicons was identical revealing identification and genotyping of only one type (Deltapapillomavirus 4 isolate EGY 2017) with accession number (MG547343) which found to be closely related to the recently detected Deltapapillomavirus 4 isolate 04_asi_UK (accession no. MF384288.1) and isolate Deltapapillomavirus 4 isolate 25_equ_CH (accession no. MF384286.1) with 99% nucleotide sequence identity. Histopathological examination revealed severe hyperkeratosis in stratum corneum and acanthosis in most of the cases. These tissue changes were confirmed by the presence of golden brown stained proliferating cell nuclear antigen which was localized intranuclear and perinuclear in other cells using IHC Technique.Conclusion:It is the first time to detect and genotype the BPVs in these areas with no record of previous genotyping in the whole country. The obtained results will highlight the importance of this disease.

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“…PV infections are known to be associated with the formation of benign skin tumors, known as papillomas, warts, and fibropapillomas in cattle. 11 It is known that BPV infection is common in Turkey 12,13 and leads to economic losses in the dairy cattle industry.…”

Section: Resultsmentioning

confidence: 99%

Detection and whole genomic characterization of <em>Bovine Papillomavirus</em> type 1 associated with severe mammary cutaneous warts in Turkey

et al. 2019

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Papilloma viruses (PVs) induce epithelial tumors in their natural hosts. These tumors are located on the mammary gland of cattle; therefore, PVs are considered viral agents with significant effects, both economical and on the animals’ health. Also, knowing the diversity of bovine PV (BPV) and its prevalence in countries worldwide is important to develop optimal diagnostic methods, as well as methods to fight this infection.The aim of the present study was to characterize the full-length genome of BPV 1 as the dominant type circulating among cattle in Turkey. It is the authors’ opinion that the results of this work provide useful information for further studies on the preventative steps that can be taken against BPV infection.

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Genomic comparison of bovine papillomavirus 1 isolates from bovine, equine and asinine lesional tissue samples

Cited by 10 publications

References 27 publications

Bovine Papillomavirus 1 Gets Out of the Flock: Detection in an Ovine Wart in Sicily

Bovine Papillomavirus 1 Gets Out of the Flock: Detection in an Ovine Wart in Sicily

Molecular detection and immunopathological examination of Deltapapillomavirus 4 in skin and udder of Egyptian cattle

Detection and whole genomic characterization of <em>Bovine Papillomavirus</em> type 1 associated with severe mammary cutaneous warts in Turkey

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