Genomic constitution, allopolyploidy, and evolutionary proposal for Cynodon Rich. based on GISH

Chaves, Ana Luisa Arantes; Mendes, Carvalho, Pedro Henrique; Ferreira, Marco Túlio Mendes; Benites, Flávio Rodrigo Gandolfi; Techio, Vânia Helena

doi:10.1007/s00709-021-01716-z

Cited by 7 publications

(2 citation statements)

References 103 publications

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“…Flow cytometry has been extensively used in plant genetics, plant physiology, and other fields (Ochatt, 2008). Genomic in situ hybridization (GISH) has been considered to be an effective and illustrative method for the rapid and reliable identification of genomic homology in allopolyploid and related species, alien chromatin in interspecific and intergeneric species, and identification of genetic constitution on chromosome level in polyploid species (Qie et al, 2007;Badaeva et al, 2021;Chaves et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Altitudinal Patterns in Adaptive Evolution of Genome Size and Inter-Genome Hybridization Between Three Elymus Species From the Qinghai–Tibetan Plateau

et al. 2022

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Genome size variation and hybridization occur frequently within or between plant species under diverse environmental conditions, which enrich species diversification and drive the evolutionary process. Elymus L. is the largest genus in Triticeae with five recognized basic genomes (St, H, P, W, and Y). However, the data on population cytogenetics of Elymus species are sparse, especially whether genome hybridization and chromosomal structure can be affected by altitude are still unknown. In order to explore the relationship between genome sizes, we studied interspecific hybridization and altitude of Elymus species at population genetic and cytological levels. Twenty-seven populations at nine different altitudes (2,800–4,300 m) of three Elymus species, namely, hexaploid E. nutans (StHY, 2n = 6x = 42), tetraploid E. burchan-buddae (StY, 2n = 4x = 28), and E. sibiricus (StH, 2n = 4x = 28), were sampled from the Qinghai–Tibetan Plateau (QTP) to estimate whether intraspecific variation could affect the genomic relationships by genomic in situ hybridization (GISH), and quantify the genome size of Elymus among different altitude ecological groups by flow cytometry. The genome size of E. nutans, E. burchan-buddae, and E. sibiricus varied from 12.38 to 22.33, 8.81 to 18.93, and 11.46 to 20.96 pg/2C with the averages of 19.59, 12.39, and 16.85 pg/2C, respectively. The curve regression analysis revealed a strong correlation between altitude and nuclear DNA content in three Elymus species. In addition, the chromosomes of the St and Y genomes demonstrated higher polymorphism than that of the H genome. Larger genome size variations occurred in the mid-altitude populations (3,900–4,300 m) compared with other-altitude populations, suggesting a notable altitudinal pattern in genome size variation, which shaped genome evolution by altitude. This result supports our former hypothesis that genetic richness center at medium altitude is useful and valuable for species adaptation to highland environmental conditions, germplasm utilization, and conservation.

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Section: Introductionmentioning

confidence: 99%

Altitudinal Patterns in Adaptive Evolution of Genome Size and Inter-Genome Hybridization Between Three Elymus Species From the Qinghai–Tibetan Plateau

et al. 2022

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“…A majority of the common bermudagrass germplasm in breeding programs are tetraploids ( Mutlu et al., 2014 ). Although the classification of C. dactylon species as allotetraploid versus autotetraploid is debated ( Harlan and De Wet, 1969 ; Fang et al., 2020 ; Chaves et al., 2022 ; Bethel et al., 2006 ; Harris-Shultz et al., 2010 ; Guo et al., 2015 ; Khanal et al., 2017 ), a recent high-density genetic map developed using genotyping-by-sequencing (GBS) provides a clearer interpretation of the C. dactylon allotetraploid structure ( Fang et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and population structure of bermudagrass (Cynodon spp.) revealed by genotyping-by-sequencing

Singh

McCurdy

et al. 2023

Front. Plant Sci.

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Bermudagrass (Cynodon spp.) breeding and cultivar development is hampered by limited information regarding its genetic and phenotypic diversity. To explore diversity in bermudagrass, a total of 206 Cynodon accessions consisting of 193 common bermudagrass (C. dactylon var. dactylon) and 13 African bermudagrass (C. transvaalensis) accessions of worldwide origin were assembled for genetic characterization. Genotyping-by-sequencing (GBS) was employed for genetic marker development. With a minor allele frequency of 0.05 and a minimum call rate of 0.5, a total of 37,496 raw single nucleotide polymorphisms (SNPs) were called de novo and were used in the genetic diversity characterization. Population structure analysis using ADMIXTURE revealed four subpopulations in this germplasm panel, which was consistent with principal component analysis (PCA) and phylogenetic analysis results. The first three principal components explained 15.6%, 10.1%, and 3.8% of the variance in the germplasm panel, respectively. The first subpopulation consisted of C. dactylon accessions from various continents; the second subpopulation was comprised mainly of C. transvaalensis accessions; the third subpopulation contained C. dactylon accessions primarily of African origin; and the fourth subpopulation represented C. dactylon accessions obtained from the Oklahoma State University bermudagrass breeding program. Genetic diversity parameters including Nei’s genetic distance, inbreeding coefficient, and Fst statistic revealed substantial genetic variation in the Cynodon accessions, demonstrating the potential of this germplasm panel for further genetic studies and cultivar development in breeding programs.

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Evaluation of zinc tolerance and accumulation in eight cultivars of bermudagrass (Cynodon spp.): implications for zinc phytoremediation

Zhang,

Sun,

Chen

et al. 2023

Biometals

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Genomic constitution, allopolyploidy, and evolutionary proposal for Cynodon Rich. based on GISH

Cited by 7 publications

References 103 publications

Altitudinal Patterns in Adaptive Evolution of Genome Size and Inter-Genome Hybridization Between Three Elymus Species From the Qinghai–Tibetan Plateau

Altitudinal Patterns in Adaptive Evolution of Genome Size and Inter-Genome Hybridization Between Three Elymus Species From the Qinghai–Tibetan Plateau

Genetic diversity and population structure of bermudagrass (Cynodon spp.) revealed by genotyping-by-sequencing

Evaluation of zinc tolerance and accumulation in eight cultivars of bermudagrass (Cynodon spp.): implications for zinc phytoremediation

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